Zinc in PDB 1c8y: Endo-Beta-N-Acetylglucosaminidase H, D130A Mutant

All present enzymatic activity of Endo-Beta-N-Acetylglucosaminidase H, D130A Mutant:
3.2.1.96;

The structure of Endo-Beta-N-Acetylglucosaminidase H, D130A Mutant, PDB code: 1c8y was solved by V.Rao, T.Cui, C.Guan, P.Van Roey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.00
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	61.400, 61.400, 140.900, 90.00, 90.00, 90.00
R / Rfree (%)	20.7 / n/a

The binding sites of Zinc atom in the Endo-Beta-N-Acetylglucosaminidase H, D130A Mutant (pdb code 1c8y). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Endo-Beta-N-Acetylglucosaminidase H, D130A Mutant, PDB code: 1c8y:

Zinc binding site 1 out of 1 in the Endo-Beta-N-Acetylglucosaminidase H, D130A Mutant


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Endo-Beta-N-Acetylglucosaminidase H, D130A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn271 b:21.6 occ:1.00 O A:HOH557 1.6 40.8 1.0 OE2 A:GLU135 2.1 34.1 1.0 OE1 A:GLU135 2.4 28.4 1.0 ND1 A:HIS94 2.4 22.1 1.0 CD A:GLU135 2.5 28.6 1.0 CG A:HIS94 3.2 22.5 1.0 CB A:HIS94 3.3 20.3 1.0 CE1 A:HIS94 3.4 22.4 1.0 O A:HOH530 3.9 21.0 1.0 CG A:GLU135 3.9 26.1 1.0 O A:HOH348 4.1 20.0 1.0 O A:HOH346 4.1 45.0 1.0 CD2 A:HIS94 4.3 22.0 1.0 CA A:HIS94 4.3 19.0 1.0 NE2 A:HIS94 4.4 23.0 1.0 CB A:GLU135 4.9 23.1 1.0

V.Rao, T.Cui, C.Guan, P.Van Roey. Mutations of Endo-Beta-N-Acetylglucosaminidase H Active Site Residues ASP130 and GLU132: Activities and Conformations. Protein Sci. V. 8 2338 1999.
ISSN: ISSN 0961-8368
PubMed: 10595536