Zinc in PDB 8c8u: Priestia Megaterium Mupirocin-Resistant Isoleucyl-Trna Synthetase 2 Complexed with Mupirocin

All present enzymatic activity of Priestia Megaterium Mupirocin-Resistant Isoleucyl-Trna Synthetase 2 Complexed with Mupirocin:
6.1.1.5;

The structure of Priestia Megaterium Mupirocin-Resistant Isoleucyl-Trna Synthetase 2 Complexed with Mupirocin, PDB code: 8c8u was solved by A.Brkic, M.Leibundgut, J.Jablonska, V.Zanki, Z.Car, V.Petrovic Perokovic, I.Gruic-Sovulj, N.Ban, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.23 / 1.90
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	89.58, 124.83, 114.46, 90, 90, 90
R / Rfree (%)	18.3 / 22.2

The binding sites of Zinc atom in the Priestia Megaterium Mupirocin-Resistant Isoleucyl-Trna Synthetase 2 Complexed with Mupirocin (pdb code 8c8u). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Priestia Megaterium Mupirocin-Resistant Isoleucyl-Trna Synthetase 2 Complexed with Mupirocin, PDB code: 8c8u:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Priestia Megaterium Mupirocin-Resistant Isoleucyl-Trna Synthetase 2 Complexed with Mupirocin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Priestia Megaterium Mupirocin-Resistant Isoleucyl-Trna Synthetase 2 Complexed with Mupirocin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1101 b:45.0 occ:1.00 SG A:CYS500 2.3 39.3 1.0 SG A:CYS459 2.3 37.6 1.0 SG A:CYS503 2.4 48.3 1.0 SG A:CYS462 2.4 48.6 1.0 CB A:CYS459 3.2 40.1 1.0 CB A:CYS500 3.2 45.4 1.0 CB A:CYS503 3.6 47.9 1.0 CB A:CYS462 3.7 43.7 1.0 N A:CYS503 3.8 43.0 1.0 N A:CYS462 3.8 53.2 1.0 CA A:CYS503 4.2 47.8 1.0 CB A:VAL502 4.2 43.5 1.0 CA A:CYS462 4.3 54.2 1.0 CB A:HIS464 4.4 37.6 1.0 CA A:CYS500 4.6 44.6 1.0 CA A:CYS459 4.6 42.0 1.0 N A:GLY505 4.7 42.8 1.0 C A:CYS503 4.8 48.5 1.0 C A:VAL502 4.8 51.2 1.0 C A:GLY461 4.8 57.0 1.0 CG1 A:VAL502 4.9 51.2 1.0 N A:SER504 4.9 46.0 1.0 C A:CYS462 4.9 50.6 1.0 CA A:GLY461 4.9 50.8 1.0 CA A:VAL502 4.9 44.0 1.0 N A:VAL502 4.9 46.5 1.0 N A:HIS464 5.0 43.0 1.0

Zinc binding site 2 out of 2 in the Priestia Megaterium Mupirocin-Resistant Isoleucyl-Trna Synthetase 2 Complexed with Mupirocin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Priestia Megaterium Mupirocin-Resistant Isoleucyl-Trna Synthetase 2 Complexed with Mupirocin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1103 b:60.5 occ:1.00 SG A:CYS387 2.2 60.1 1.0 SG A:CYS184 2.2 59.8 1.0 SG A:CYS390 2.5 61.5 1.0 SG A:CYS181 2.6 46.9 1.0 CB A:CYS387 3.1 61.0 1.0 CB A:CYS390 3.6 61.6 1.0 CB A:CYS181 3.7 55.1 1.0 CB A:CYS184 3.7 50.3 1.0 N A:CYS390 3.8 63.8 1.0 N A:CYS184 3.9 50.9 1.0 CB A:ARG389 4.2 65.7 1.0 CA A:CYS390 4.2 61.6 1.0 CB A:SER183 4.3 60.5 1.0 C A:ARG389 4.3 71.3 1.0 CA A:CYS184 4.4 57.3 1.0 CA A:CYS387 4.6 67.7 1.0 CA A:ARG389 4.6 65.6 1.0 N A:ARG389 4.8 68.9 1.0 C A:SER183 4.8 54.2 1.0 OG A:SER183 4.9 66.0 1.0 CB A:SER392 4.9 66.5 1.0 C A:CYS390 5.0 65.8 1.0 O A:CYS387 5.0 66.3 1.0 O A:ARG389 5.0 65.1 1.0 C A:CYS387 5.0 72.4 1.0 CA A:SER183 5.0 58.9 1.0

A.Brkic, M.Leibundgut, J.Jablonska, V.Zanki, Z.Car, V.Petrovic Perokovic, N.Ban, I.Gruic-Sovulj. Antibiotic Hyper-Resistance in A Class I Aminoacyl-Trna Synthetase with Altered Active Site Signature Motif To Be Published.