Zinc in PDB 5ynj: Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to M7GPPPG

The structure of Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to M7GPPPG, PDB code: 5ynj was solved by S.M.Wei, L.Yang, Z.H.Ke, Y.Chen, D.Y.Guo, C.P.Fan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.46 / 2.04
Space group	P 21 2 21
Cell size a, b, c (Å), α, β, γ (°)	67.041, 70.516, 118.921, 90.00, 90.00, 90.00
R / Rfree (%)	19.5 / 22.1

The binding sites of Zinc atom in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to M7GPPPG (pdb code 5ynj). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to M7GPPPG, PDB code: 5ynj:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to M7GPPPG


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to M7GPPPG within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:48.1 occ:1.00 NE2 B:HIS83 2.1 49.9 1.0 SG B:CYS77 2.3 47.4 1.0 SG B:CYS90 2.3 53.2 1.0 SG B:CYS74 2.4 49.6 1.0 CD2 B:HIS83 2.9 50.5 1.0 CB B:CYS90 3.1 56.5 1.0 CE1 B:HIS83 3.2 50.8 1.0 CB B:CYS74 3.2 46.1 1.0 CB B:CYS77 3.4 47.2 1.0 N B:CYS77 3.6 50.0 1.0 CA B:CYS77 4.0 49.9 1.0 CG B:HIS83 4.1 49.2 1.0 ND1 B:HIS83 4.2 51.5 1.0 CA B:CYS90 4.4 58.2 1.0 C B:TYR76 4.4 53.9 1.0 CB B:TYR76 4.6 50.0 1.0 CB B:LYS93 4.6 44.5 1.0 CA B:CYS74 4.6 46.9 1.0 O B:HOH315 4.8 50.2 1.0 CA B:TYR76 4.9 50.5 1.0 N B:LYS93 4.9 45.6 1.0 N B:TYR76 5.0 51.1 1.0 C B:CYS74 5.0 48.0 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to M7GPPPG


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to M7GPPPG within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn202 b:77.7 occ:1.00 CB B:CYS120 2.1 74.7 1.0 SG B:CYS130 2.3 92.7 1.0 SG B:CYS117 2.4 80.7 1.0 SG B:CYS120 2.4 68.9 1.0 SG B:CYS128 2.4 75.1 1.0 CB B:CYS117 3.0 76.8 1.0 CB B:CYS128 3.3 73.9 1.0 CA B:CYS120 3.6 74.6 1.0 CB B:CYS130 3.7 97.4 1.0 CA B:CYS128 3.8 77.1 1.0 N B:CYS120 4.1 79.7 1.0 N B:ASN129 4.1 86.5 1.0 N B:CYS130 4.1 96.7 1.0 C B:CYS128 4.3 80.5 1.0 C B:CYS120 4.3 84.5 1.0 CA B:CYS117 4.5 80.3 1.0 CG2 B:VAL119 4.5 93.0 1.0 CA B:CYS130 4.6 99.1 1.0 N B:GLN121 4.9 90.0 1.0 O B:CYS120 4.9 81.2 1.0 C B:ASN129 5.0 97.2 1.0

S.M.Wei, L.Yang, Z.H.Ke, Q.Y.Liu, Z.Z.Yang, Y.Chen, D.Y.Guo, C.P.Fa. Structural Insights Into the Molecular Mechanism of Mers Coronavirus Rna Ribose 2'-O-Methylation By NSP16/NSP10 Protein Complex To Be Published.