Zinc in PDB 9g0l: Crystal Structure of the Ring-ZNF1 Fragment of SIAH1

All present enzymatic activity of Crystal Structure of the Ring-ZNF1 Fragment of SIAH1:
2.3.2.27;

The structure of Crystal Structure of the Ring-ZNF1 Fragment of SIAH1, PDB code: 9g0l was solved by F.Coste, M.J.Suskiewicz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.66 / 1.90
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	69.85, 69.85, 62.713, 90, 90, 90
R / Rfree (%)	20.2 / 23.7

The binding sites of Zinc atom in the Crystal Structure of the Ring-ZNF1 Fragment of SIAH1 (pdb code 9g0l). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Crystal Structure of the Ring-ZNF1 Fragment of SIAH1, PDB code: 9g0l:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Crystal Structure of the Ring-ZNF1 Fragment of SIAH1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Ring-ZNF1 Fragment of SIAH1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn201 b:37.6 occ:1.00 ND1 A:HIS59 2.1 39.2 1.0 SG A:CYS72 2.3 38.2 1.0 SG A:CYS55 2.3 36.3 1.0 SG A:CYS75 2.3 40.1 1.0 CG A:HIS59 3.0 38.9 1.0 CE1 A:HIS59 3.1 40.1 1.0 CB A:CYS72 3.2 38.0 1.0 CB A:HIS59 3.3 38.2 1.0 CB A:CYS75 3.3 37.4 1.0 CB A:CYS55 3.4 36.4 1.0 N A:CYS75 3.7 44.0 1.0 CA A:CYS75 4.1 43.0 1.0 CD2 A:HIS59 4.2 39.5 1.0 NE2 A:HIS59 4.2 40.4 1.0 N A:HIS59 4.4 40.4 1.0 CA A:HIS59 4.4 36.9 1.0 O A:HOH303 4.5 42.9 1.0 CB A:THR74 4.5 40.0 1.0 CA A:CYS72 4.7 43.6 1.0 CB A:SER57 4.7 37.9 1.0 C A:THR74 4.7 39.2 1.0 CA A:CYS55 4.8 35.2 1.0 O A:HOH351 4.8 47.7 1.0 C A:CYS75 4.8 38.0 1.0 CA A:THR74 5.0 41.0 1.0 N A:ARG76 5.0 41.9 1.0 N A:THR74 5.0 41.4 1.0 N A:SER57 5.0 35.7 1.0

Zinc binding site 2 out of 3 in the Crystal Structure of the Ring-ZNF1 Fragment of SIAH1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Ring-ZNF1 Fragment of SIAH1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn202 b:43.2 occ:1.00 NE2 A:HIS117 2.1 43.9 1.0 SG A:CYS121 2.3 45.7 1.0 SG A:CYS105 2.3 44.7 1.0 SG A:CYS98 2.3 44.0 1.0 CB A:CYS98 3.0 43.8 1.0 CD2 A:HIS117 3.0 36.9 1.0 CE1 A:HIS117 3.0 40.0 1.0 CB A:CYS105 3.1 47.2 1.0 CB A:CYS121 3.3 49.4 1.0 ND1 A:HIS117 4.1 38.2 1.0 CG A:HIS117 4.2 44.5 1.0 CA A:CYS121 4.4 48.1 1.0 CA A:CYS98 4.4 45.7 1.0 CA A:CYS105 4.6 50.3 1.0 CB A:TYR100 4.6 51.2 1.0 CB A:ILE107 4.6 42.7 1.0 CD2 A:TYR100 4.7 51.5 1.0 CG1 A:ILE107 5.0 44.4 1.0 CD2 A:PHE123 5.0 50.8 1.0

Zinc binding site 3 out of 3 in the Crystal Structure of the Ring-ZNF1 Fragment of SIAH1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the Ring-ZNF1 Fragment of SIAH1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn203 b:65.5 occ:1.00 SG A:CYS62 2.3 59.4 1.0 SG A:CYS44 2.3 66.3 1.0 SG A:CYS65 2.3 69.8 1.0 SG A:CYS41 2.3 58.8 1.0 CB A:CYS41 3.1 48.6 1.0 CB A:CYS65 3.3 65.3 1.0 CB A:CYS44 3.3 63.7 1.0 CB A:CYS62 3.5 55.0 1.0 N A:CYS44 3.6 63.6 1.0 CA A:CYS44 4.1 69.7 1.0 N A:CYS62 4.1 54.3 1.0 N A:CYS65 4.2 68.0 1.0 CA A:CYS62 4.3 55.6 1.0 CA A:CYS65 4.4 64.8 1.0 CB A:VAL43 4.5 60.1 1.0 CA A:CYS41 4.6 47.3 1.0 C A:VAL43 4.6 62.5 1.0 CG1 A:VAL43 4.7 64.8 1.0 C A:CYS44 4.8 69.2 1.0 N A:PHE45 4.9 68.4 1.0 C A:CYS62 4.9 59.4 1.0 CA A:VAL43 4.9 60.3 1.0 N A:VAL43 4.9 58.2 1.0 O A:CYS62 5.0 58.9 1.0

F.Coste, A.Mishra, C.Chapuis, L.Mance, Z.Pukalo, N.Bigot, S.Goffinont, V.Gaudon, N.Garnier, I.Talhaoui, B.Castaing, S.Huet, M.J.Suskiewicz. Ring Dimerisation Drives Higher-Order Organisation of Sina/Siah E3 Ubiquitin Ligases. Febs J. 2025.
ISSN: ISSN 1742-464X
PubMed: 39910688
DOI: 10.1111/FEBS.70000