Zinc in PDB 5yni: Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sam and M7GPPPG

The structure of Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sam and M7GPPPG, PDB code: 5yni was solved by S.M.Wei, L.Yang, Z.H.Ke, Y.Chen, D.Y.Guo, C.P.Fan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.41 / 2.07
Space group	P 21 2 21
Cell size a, b, c (Å), α, β, γ (°)	66.954, 70.064, 119.762, 90.00, 90.00, 90.00
R / Rfree (%)	19.4 / 23.7

The binding sites of Zinc atom in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sam and M7GPPPG (pdb code 5yni). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sam and M7GPPPG, PDB code: 5yni:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sam and M7GPPPG


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sam and M7GPPPG within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:44.3 occ:1.00 NE2 B:HIS83 2.1 45.0 1.0 SG B:CYS74 2.3 42.6 1.0 SG B:CYS90 2.3 50.0 1.0 SG B:CYS77 2.3 42.6 1.0 CD2 B:HIS83 2.8 47.3 1.0 CB B:CYS74 3.2 44.0 1.0 CB B:CYS90 3.2 50.5 1.0 CE1 B:HIS83 3.3 45.1 1.0 CB B:CYS77 3.4 41.5 1.0 N B:CYS77 3.6 44.1 1.0 CA B:CYS77 4.0 43.4 1.0 CG B:HIS83 4.1 48.3 1.0 ND1 B:HIS83 4.2 47.0 1.0 CA B:CYS90 4.4 53.3 1.0 C B:TYR76 4.4 44.0 1.0 CB B:TYR76 4.5 46.4 1.0 CA B:CYS74 4.6 42.6 1.0 CB B:LYS93 4.6 42.7 1.0 N B:LYS93 4.9 41.5 1.0 CA B:TYR76 4.9 45.4 1.0 C B:CYS74 5.0 41.7 1.0 O B:CYS74 5.0 40.7 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sam and M7GPPPG


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Mers-Cov NSP16/NSP10 Complex Bound to Sam and M7GPPPG within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn202 b:76.2 occ:1.00 CB B:CYS120 2.1 75.7 1.0 SG B:CYS128 2.3 72.7 1.0 SG B:CYS130 2.3 96.8 1.0 SG B:CYS117 2.4 74.8 1.0 SG B:CYS120 2.5 69.9 1.0 CB B:CYS117 3.1 71.8 1.0 CB B:CYS128 3.5 70.7 1.0 CA B:CYS120 3.5 79.9 1.0 CB B:CYS130 3.6 99.8 1.0 N B:CYS120 3.9 81.7 1.0 CA B:CYS128 4.1 72.0 1.0 O B:HOH316 4.3 76.5 1.0 N B:ASN129 4.3 84.5 1.0 C B:CYS120 4.4 80.8 1.0 N B:CYS130 4.4 99.6 1.0 C B:CYS128 4.5 76.3 1.0 CA B:CYS117 4.6 71.9 1.0 CA B:CYS130 4.6 0.8 1.0 C B:ASN129 4.8 97.0 1.0 O B:CYS120 4.9 80.6 1.0 N B:GLN121 5.0 82.5 1.0

S.M.Wei, L.Yang, Z.H.Ke, Q.Y.Liu, Z.Z.Yang, Y.Chen, D.Y.Guo, C.P.Fan. Structural Insights Into the Molecular Mechanism of Mers Coronavirus Rna Ribose 2'-O-Methylation By NSP16/NSP10 Protein Complex To Be Published.