Zinc in PDB 5ulh: Structure of RNF165 in Complex with A UBCH5B~Ub Conjugate

All present enzymatic activity of Structure of RNF165 in Complex with A UBCH5B~Ub Conjugate:
2.3.2.23; 2.3.2.24; 2.3.2.27;

The structure of Structure of RNF165 in Complex with A UBCH5B~Ub Conjugate, PDB code: 5ulh was solved by A.J.Middleton, C.L.Day, J.D.Wright, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	56.17 / 1.95
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	58.278, 60.081, 112.339, 90.00, 90.00, 90.00
R / Rfree (%)	18.9 / 21.7

The binding sites of Zinc atom in the Structure of RNF165 in Complex with A UBCH5B~Ub Conjugate (pdb code 5ulh). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of RNF165 in Complex with A UBCH5B~Ub Conjugate, PDB code: 5ulh:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of RNF165 in Complex with A UBCH5B~Ub Conjugate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of RNF165 in Complex with A UBCH5B~Ub Conjugate within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn401 b:33.6 occ:1.00 CD2 C:HIS314 2.0 32.1 1.0 SG C:CYS312 2.2 32.7 1.0 SG C:CYS331 2.3 27.9 1.0 SG C:CYS334 2.3 35.4 1.0 CB C:CYS331 2.9 26.8 1.0 CG C:HIS314 3.0 33.5 1.0 NE2 C:HIS314 3.1 38.5 1.0 CB C:CYS312 3.1 37.5 1.0 CB C:CYS334 3.4 39.2 1.0 CB C:HIS314 3.5 31.2 1.0 N C:CYS334 3.7 34.0 1.0 CA C:CYS334 4.1 37.6 1.0 ND1 C:HIS314 4.1 37.4 1.0 CE1 C:HIS314 4.2 36.8 1.0 CA C:CYS331 4.3 27.1 1.0 N C:HIS314 4.4 36.8 1.0 CA C:CYS312 4.5 41.2 1.0 CA C:HIS314 4.6 33.2 1.0 CB C:ILE333 4.6 35.7 1.0 C C:CYS312 4.7 42.7 1.0 O C:CYS312 4.7 44.2 1.0 C C:ILE333 4.8 33.9 1.0 C C:CYS334 4.8 37.0 1.0 C C:CYS331 4.8 27.2 1.0 N C:ARG335 4.9 35.4 1.0 N C:ILE333 4.9 29.8 1.0

Zinc binding site 2 out of 2 in the Structure of RNF165 in Complex with A UBCH5B~Ub Conjugate


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of RNF165 in Complex with A UBCH5B~Ub Conjugate within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn402 b:28.8 occ:1.00 CD2 C:HIS317 2.2 24.3 1.0 SG C:CYS297 2.2 28.9 1.0 SG C:CYS320 2.2 26.1 1.0 SG C:CYS294 2.2 26.8 1.0 CG C:HIS317 3.0 25.1 1.0 CB C:CYS294 3.0 27.8 1.0 CB C:CYS297 3.2 29.5 1.0 CB C:CYS320 3.2 25.2 1.0 NE2 C:HIS317 3.3 30.6 1.0 CB C:HIS317 3.3 22.6 1.0 N C:CYS297 3.5 25.2 1.0 CA C:CYS297 3.9 25.7 1.0 N C:HIS317 4.1 24.1 1.0 ND1 C:HIS317 4.2 28.4 1.0 CA C:HIS317 4.3 24.2 1.0 CE1 C:HIS317 4.3 27.2 1.0 CA C:CYS294 4.4 29.0 1.0 CA C:CYS320 4.6 26.0 1.0 C C:ILE296 4.6 23.1 1.0 CB C:ILE296 4.6 24.0 1.0 C C:CYS294 4.7 27.0 1.0 O C:CYS294 4.8 26.7 1.0 C C:CYS297 4.8 28.6 1.0 N C:LEU298 4.9 28.3 1.0 N C:ILE296 4.9 24.7 1.0 N C:CYS320 4.9 23.3 1.0 CA C:ILE296 5.0 24.1 1.0

A.J.Middleton, J.D.Wright, C.L.Day. Discovery of New Non-Covalent Ubiquitin Binding Sites on UBCH5 To Be Published.