Zinc in PDB 5ui8: Structure of Sigman-Holoenzyme

All present enzymatic activity of Structure of Sigman-Holoenzyme:
2.7.7.6;

The structure of Structure of Sigman-Holoenzyme, PDB code: 5ui8 was solved by S.A.Darst, E.A.Campbell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.64 / 3.76
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	208.476, 151.524, 195.279, 90.00, 90.00, 90.00
R / Rfree (%)	26.2 / 31.2

The structure of Structure of Sigman-Holoenzyme also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Structure of Sigman-Holoenzyme (pdb code 5ui8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Sigman-Holoenzyme, PDB code: 5ui8:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Sigman-Holoenzyme


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Sigman-Holoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:0.9 occ:1.00 SG J:CYS70 2.3 0.1 1.0 SG J:CYS72 2.3 0.3 1.0 SG J:CYS85 2.3 0.8 1.0 SG J:CYS88 2.8 0.2 1.0 O J:CYS88 3.2 0.0 1.0 CB J:CYS72 3.3 0.9 1.0 N J:CYS88 3.5 0.5 1.0 CB J:CYS85 3.6 0.7 1.0 CB J:CYS70 3.7 0.9 1.0 CB J:CYS88 3.8 0.3 1.0 N J:CYS72 3.9 0.3 1.0 CA J:CYS88 4.0 0.8 1.0 C J:CYS88 4.0 0.4 1.0 CA J:CYS72 4.2 0.3 1.0 N J:LYS87 4.4 0.2 1.0 C J:LYS87 4.5 0.8 1.0 CA J:CYS85 4.6 0.7 1.0 N J:GLY73 4.7 0.1 1.0 N J:LEU71 4.8 0.9 1.0 CA J:LYS87 4.8 0.9 1.0 CB J:LYS87 4.8 1.0 1.0 CD1 J:TYR75 4.8 0.8 1.0 N J:GLU86 4.9 0.5 1.0 CA J:CYS70 4.9 0.8 1.0 C J:CYS72 4.9 0.4 1.0

Zinc binding site 2 out of 2 in the Structure of Sigman-Holoenzyme


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Sigman-Holoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1503 b:0.4 occ:1.00 SG J:CYS898 2.3 0.6 1.0 SG J:CYS888 2.4 0.0 1.0 SG J:CYS814 2.4 0.7 1.0 CB J:CYS898 3.0 0.7 1.0 SG J:CYS895 3.2 0.9 1.0 CB J:CYS888 3.3 0.5 1.0 CA J:CYS888 3.4 0.9 1.0 CB J:CYS895 3.5 0.3 1.0 NH2 J:ARG883 3.8 0.9 1.0 N J:ASP889 3.8 0.5 1.0 CB J:CYS814 4.0 0.7 1.0 CG2 J:THR816 4.1 0.8 1.0 C J:CYS888 4.1 0.8 1.0 CA J:CYS898 4.3 0.1 1.0 N J:CYS898 4.5 0.0 1.0 N J:CYS895 4.6 0.4 1.0 CA J:CYS895 4.6 0.9 1.0 N J:CYS888 4.6 0.4 1.0 N J:CYS814 4.7 0.9 1.0 CZ J:ARG883 4.7 0.9 1.0 NE J:ARG883 4.8 0.2 1.0 CG2 J:THR890 4.9 0.0 1.0 N J:THR890 4.9 1.0 1.0 CA J:CYS814 4.9 0.1 1.0

E.A.Campbell, S.Kamath, K.R.Rajashankar, M.Wu, S.A.Darst. Crystal Structure of Aquifex Aeolicus Sigma (N) Bound to Promoter Dna and the Structure of Sigma (N)-Holoenzyme. Proc. Natl. Acad. Sci. V. 114 E1805 2017U.S.A..
ISSN: ESSN 1091-6490
PubMed: 28223493
DOI: 10.1073/PNAS.1619464114