Atomistry » Zinc » PDB 5lsx-5m2h » 5lu7
Atomistry »
  Zinc »
    PDB 5lsx-5m2h »
      5lu7 »

Zinc in PDB 5lu7: Heptose Isomerase Gmha Mutant - D61A

Enzymatic activity of Heptose Isomerase Gmha Mutant - D61A

All present enzymatic activity of Heptose Isomerase Gmha Mutant - D61A:
5.3.1.28;

Protein crystallography data

The structure of Heptose Isomerase Gmha Mutant - D61A, PDB code: 5lu7 was solved by M.Vivoli, N.J.Harmer, J.Pang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 37.54 / 1.92
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 73.200, 83.680, 126.100, 90.00, 90.00, 90.00
R / Rfree (%) 17 / 21.5

Zinc Binding Sites:

The binding sites of Zinc atom in the Heptose Isomerase Gmha Mutant - D61A (pdb code 5lu7). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Heptose Isomerase Gmha Mutant - D61A, PDB code: 5lu7:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in 5lu7

Go back to Zinc Binding Sites List in 5lu7
Zinc binding site 1 out of 4 in the Heptose Isomerase Gmha Mutant - D61A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Heptose Isomerase Gmha Mutant - D61A within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn201

b:20.8
occ:0.23
 OE2 A:GLU68 1.9 29.3 1.0
NE2 A:HIS64 1.9 21.2 1.0
NE2 A:HIS183 1.9 19.6 1.0
OE1 D:GLN175 2.1 19.6 1.0
CE1 A:HIS64 2.8 24.4 1.0
CD A:GLU68 2.8 28.9 1.0
CD2 A:HIS183 2.8 20.1 1.0
CE1 A:HIS183 3.0 17.2 1.0
CD2 A:HIS64 3.0 20.2 1.0
OE1 A:GLU68 3.2 34.9 1.0
CD D:GLN175 3.3 23.1 1.0
O3 D:M7P201 3.6 27.8 1.0
NE2 D:GLN175 3.8 15.7 1.0
ND1 A:HIS64 4.0 22.6 1.0
CG A:HIS183 4.0 21.5 1.0
ND1 A:HIS183 4.1 18.5 1.0
CG A:GLU68 4.1 21.6 1.0
CG A:HIS64 4.1 20.9 1.0
CD1 A:LEU179 4.4 18.5 1.0
CG D:GLN175 4.5 18.7 1.0
O D:HOH352 4.6 34.6 1.0
CB D:GLN175 4.6 17.2 1.0
CA D:GLY57 4.8 17.5 1.0
CE1 A:PHE73 5.0 25.2 1.0
CA D:PRO172 5.0 17.2 1.0
C3 D:M7P201 5.0 21.1 1.0

Zinc binding site 2 out of 4 in 5lu7

Go back to Zinc Binding Sites List in 5lu7
Zinc binding site 2 out of 4 in the Heptose Isomerase Gmha Mutant - D61A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Heptose Isomerase Gmha Mutant - D61A within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn205

b:26.3
occ:0.28
 OE2 D:GLU68 1.9 32.3 1.0
OE1 A:GLN175 1.9 20.9 1.0
NE2 D:HIS183 2.0 16.6 1.0
NE2 D:HIS64 2.1 26.0 1.0
CE1 D:HIS183 2.9 19.3 1.0
CD D:GLU68 3.0 29.6 1.0
CD2 D:HIS64 3.0 24.8 1.0
CE1 D:HIS64 3.1 22.3 1.0
CD A:GLN175 3.1 20.5 1.0
CD2 D:HIS183 3.1 20.9 1.0
OE1 D:GLU68 3.6 33.0 1.0
NE2 A:GLN175 3.7 18.8 1.0
O3 A:M7P202 3.8 30.1 1.0
ND1 D:HIS183 4.1 15.6 1.0
ND1 D:HIS64 4.1 24.8 1.0
CG D:HIS64 4.2 25.0 1.0
CG D:HIS183 4.2 20.2 1.0
CG D:GLU68 4.2 24.4 1.0
CG A:GLN175 4.3 17.0 1.0
CB A:GLN175 4.4 18.6 1.0
CA A:PRO172 4.6 18.3 1.0
CD1 D:LEU179 4.6 20.6 1.0
CE1 D:PHE73 4.8 21.5 1.0
CA A:GLY57 4.8 18.5 1.0
CB A:PRO172 4.8 25.4 1.0

Zinc binding site 3 out of 4 in 5lu7

Go back to Zinc Binding Sites List in 5lu7
Zinc binding site 3 out of 4 in the Heptose Isomerase Gmha Mutant - D61A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Heptose Isomerase Gmha Mutant - D61A within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn201

b:25.8
occ:0.21
 OE1 B:GLU68 1.9 33.7 1.0
OE1 C:GLN175 1.9 19.8 1.0
NE2 B:HIS183 2.0 27.7 1.0
NE2 B:HIS64 2.1 24.5 1.0
O B:HOH302 2.6 33.4 1.0
O C:HOH301 3.0 30.9 1.0
CD2 B:HIS183 3.0 27.3 1.0
CD B:GLU68 3.0 37.1 1.0
CE1 B:HIS183 3.0 19.4 1.0
CE1 B:HIS64 3.0 27.6 1.0
CD C:GLN175 3.0 24.2 1.0
CD2 B:HIS64 3.1 22.8 1.0
OE2 B:GLU68 3.5 40.1 1.0
NE2 C:GLN175 3.6 20.6 1.0
O3 C:M7P201 3.9 26.0 1.0
ND1 B:HIS183 4.1 25.9 1.0
CG B:HIS183 4.1 27.9 1.0
ND1 B:HIS64 4.1 24.8 1.0
CG B:HIS64 4.2 27.0 1.0
CG B:GLU68 4.2 26.6 1.0
CG C:GLN175 4.3 20.0 1.0
CB C:GLN175 4.4 18.4 1.0
CA C:PRO172 4.5 22.4 1.0
CD1 B:LEU179 4.6 22.1 1.0
CB C:PRO172 4.6 29.4 1.0
CA C:GLY57 4.8 19.7 1.0
CE1 B:PHE73 4.8 28.5 1.0

Zinc binding site 4 out of 4 in 5lu7

Go back to Zinc Binding Sites List in 5lu7
Zinc binding site 4 out of 4 in the Heptose Isomerase Gmha Mutant - D61A


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Heptose Isomerase Gmha Mutant - D61A within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn203

b:31.3
occ:0.31
 OE2 C:GLU68 1.9 33.9 1.0
NE2 C:HIS183 2.0 26.9 1.0
OE1 B:GLN175 2.1 22.7 1.0
NE2 C:HIS64 2.1 23.6 1.0
O C:HOH304 2.7 31.1 1.0
CD C:GLU68 2.8 30.1 1.0
CD2 C:HIS183 2.9 17.7 1.0
CD2 C:HIS64 3.0 22.3 1.0
CE1 C:HIS64 3.1 28.4 1.0
CD B:GLN175 3.1 23.2 1.0
CE1 C:HIS183 3.1 25.1 1.0
OE1 C:GLU68 3.2 38.2 1.0
NE2 B:GLN175 3.6 23.7 1.0
O3 B:M7P202 3.7 27.1 1.0
CG C:GLU68 4.0 24.3 1.0
CG C:HIS183 4.1 22.4 1.0
CG C:HIS64 4.1 25.2 1.0
ND1 C:HIS64 4.1 27.8 1.0
ND1 C:HIS183 4.2 18.7 1.0
CG B:GLN175 4.4 19.8 1.0
CB B:GLN175 4.6 20.7 1.0
CD1 C:LEU179 4.7 23.8 1.0
CA B:GLY57 4.7 19.8 1.0
CE1 C:PHE73 4.7 26.1 1.0
CA B:PRO172 4.9 22.1 1.0

Reference:

M.Vivoli, J.Pang, N.J.Harmer. A Half-Site Multimeric Enzyme Achieves Its Cooperativity Without Conformational Changes. Sci Rep V. 7 16529 2017.
ISSN: ESSN 2045-2322
PubMed: 29184087
DOI: 10.1038/S41598-017-16421-2
Page generated: Sun Oct 27 21:27:19 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy