Zinc in PDB 5hms: X-Ray Structure of Human Recombinant 5-Aminolaevulinic Acid Dehydratase (Hralad).

All present enzymatic activity of X-Ray Structure of Human Recombinant 5-Aminolaevulinic Acid Dehydratase (Hralad).:
4.2.1.24;

The structure of X-Ray Structure of Human Recombinant 5-Aminolaevulinic Acid Dehydratase (Hralad)., PDB code: 5hms was solved by D.Butler, P.T.Erskine, J.B.Cooper, P.M.Shoolingin-Jordan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.20 / 2.80
Space group	P 4 21 2
Cell size a, b, c (Å), α, β, γ (°)	127.079, 127.079, 91.231, 90.00, 90.00, 90.00
R / Rfree (%)	17.2 / 26.5

The binding sites of Zinc atom in the X-Ray Structure of Human Recombinant 5-Aminolaevulinic Acid Dehydratase (Hralad). (pdb code 5hms). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the X-Ray Structure of Human Recombinant 5-Aminolaevulinic Acid Dehydratase (Hralad)., PDB code: 5hms:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the X-Ray Structure of Human Recombinant 5-Aminolaevulinic Acid Dehydratase (Hralad).


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of X-Ray Structure of Human Recombinant 5-Aminolaevulinic Acid Dehydratase (Hralad). within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn400 b:63.8 occ:1.00 SG A:CYS122 2.2 61.0 1.0 SG A:CYS124 2.2 75.1 1.0 SG A:CYS132 2.3 63.3 1.0 CB A:CYS132 3.1 64.9 1.0 CB A:CYS122 3.3 49.2 1.0 CB A:CYS124 3.5 64.1 1.0 CA A:CYS132 3.9 62.7 1.0 OG A:SER168 4.2 38.3 1.0 O A:SER168 4.2 48.9 1.0 N A:CYS124 4.3 55.6 1.0 CA A:ASP169 4.5 52.9 1.0 N A:CYS132 4.5 67.0 1.0 CA A:CYS124 4.5 63.5 1.0 C A:SER168 4.5 43.4 1.0 CA A:CYS122 4.6 46.7 1.0 N A:ASP169 4.7 48.5 1.0 CB A:SER168 5.0 39.1 1.0

Zinc binding site 2 out of 2 in the X-Ray Structure of Human Recombinant 5-Aminolaevulinic Acid Dehydratase (Hralad).


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of X-Ray Structure of Human Recombinant 5-Aminolaevulinic Acid Dehydratase (Hralad). within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn400 b:81.1 occ:1.00 SG B:CYS122 2.3 59.1 1.0 SG B:CYS124 2.4 0.2 1.0 SG B:CYS132 2.7 0.6 1.0 CB B:CYS122 3.2 60.2 1.0 CB B:CYS132 3.2 0.9 1.0 CB B:CYS124 3.6 0.8 1.0 CA B:CYS132 3.9 0.6 1.0 CB B:SER168 4.3 47.8 1.0 N B:CYS124 4.4 90.6 1.0 N B:ASP169 4.4 50.6 1.0 CA B:ASP169 4.5 57.6 1.0 NZ B:LYS199 4.5 50.9 1.0 C B:SER168 4.5 46.9 1.0 CA B:CYS124 4.6 0.1 1.0 O B:SER168 4.6 49.5 1.0 OG B:SER168 4.7 49.1 1.0 CA B:CYS122 4.7 59.0 1.0 N B:CYS132 4.8 0.8 1.0 O B:CYS132 4.9 91.7 1.0 N B:LEU123 5.0 67.7 1.0 C B:CYS132 5.0 95.7 1.0

N.Mills-Davies, D.Butler, E.Norton, D.Thompson, M.Sarwar, J.Guo, R.Gill, N.Azim, A.Coker, S.P.Wood, P.T.Erskine, L.Coates, J.B.Cooper, N.Rashid, M.Akhtar, P.M.Shoolingin-Jordan. Structural Studies of Substrate and Product Complexes of 5-Aminolaevulinic Acid Dehydratase From Humans, Escherichia Coli and the Hyperthermophile Pyrobaculum Calidifontis. Acta Crystallogr D Struct V. 73 9 2017BIOL.
ISSN: ISSN 2059-7983
PubMed: 28045381
DOI: 10.1107/S2059798316019525