Zinc in PDB 5h9e: Crystal Structure of E. Coli Cascade Bound to A Pam-Containing Dsdna Target (32-Nt Spacer) at 3.20 Angstrom Resolution.

The structure of Crystal Structure of E. Coli Cascade Bound to A Pam-Containing Dsdna Target (32-Nt Spacer) at 3.20 Angstrom Resolution., PDB code: 5h9e was solved by R.P.Hayes, Y.Xiao, F.Ding, P.B.G.Van Erp, K.Rajashankar, S.Bailey, B.Wiedenheft, A.Ke, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.09 / 3.21
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	92.807, 149.825, 404.101, 90.00, 90.00, 90.00
R / Rfree (%)	20.9 / 24.7

The binding sites of Zinc atom in the Crystal Structure of E. Coli Cascade Bound to A Pam-Containing Dsdna Target (32-Nt Spacer) at 3.20 Angstrom Resolution. (pdb code 5h9e). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of E. Coli Cascade Bound to A Pam-Containing Dsdna Target (32-Nt Spacer) at 3.20 Angstrom Resolution., PDB code: 5h9e:

Zinc binding site 1 out of 1 in the Crystal Structure of E. Coli Cascade Bound to A Pam-Containing Dsdna Target (32-Nt Spacer) at 3.20 Angstrom Resolution.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of E. Coli Cascade Bound to A Pam-Containing Dsdna Target (32-Nt Spacer) at 3.20 Angstrom Resolution. within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn601 b:61.5 occ:1.00 SG A:CYS253 2.2 58.2 1.0 SG A:CYS143 2.2 54.7 1.0 SG A:CYS140 2.3 64.6 1.0 SG A:CYS250 2.4 58.2 1.0 CB A:CYS250 3.1 63.8 1.0 CB A:CYS253 3.2 57.9 1.0 CB A:CYS143 3.5 53.1 1.0 CB A:CYS140 3.8 63.9 1.0 N A:CYS253 4.0 54.1 1.0 CA A:CYS253 4.1 55.2 1.0 N A:CYS140 4.2 61.5 1.0 N A:CYS143 4.4 55.0 1.0 CA A:CYS143 4.5 52.7 1.0 CA A:CYS140 4.6 63.5 1.0 CA A:CYS250 4.6 62.7 1.0 CB A:CYS252 4.9 51.1 1.0 C A:CYS253 4.9 57.3 1.0 C A:CYS252 4.9 53.7 1.0

R.P.Hayes, Y.Xiao, F.Ding, P.B.Van Erp, K.Rajashankar, S.Bailey, B.Wiedenheft, A.Ke. Structural Basis For Promiscuous Pam Recognition in Type I-E Cascade From E. Coli. Nature V. 530 499 2016.
ISSN: ESSN 1476-4687
PubMed: 26863189
DOI: 10.1038/NATURE16995