Zinc in PDB 5bw8: 2.8 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae

The structure of 2.8 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae, PDB code: 5bw8 was solved by H.B.Gristick, J.W.Chartron, W.M.Clemons, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.80 / 2.80
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	111.960, 238.050, 52.190, 90.00, 90.00, 90.00
R / Rfree (%)	22.4 / 26.2

The binding sites of Zinc atom in the 2.8 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae (pdb code 5bw8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the 2.8 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae, PDB code: 5bw8:

Zinc binding site 1 out of 1 in the 2.8 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of 2.8 A Crystal Structure of A GET3-GET4-GET5 Intermediate Complex From S.Cerevisiae within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:0.1 occ:1.00 SG B:CYS285 2.2 0.1 1.0 SG B:CYS288 2.3 0.1 1.0 SG A:CYS288 2.3 94.8 1.0 SG A:CYS285 2.4 0.6 1.0 CB B:CYS285 3.0 0.1 1.0 CB B:CYS288 3.4 98.5 1.0 CB A:CYS285 3.5 0.0 1.0 CB A:CYS288 3.7 94.8 1.0 N B:CYS288 4.0 94.8 1.0 N A:CYS288 4.0 92.6 1.0 N A:CYS285 4.2 0.1 1.0 CA B:CYS285 4.2 0.5 1.0 CA B:CYS288 4.3 95.2 1.0 CA A:CYS285 4.3 0.2 1.0 CA A:CYS288 4.4 94.6 1.0 CB A:ARG287 4.6 97.4 1.0 C A:CYS285 4.7 0.6 1.0 O A:CYS285 4.7 0.3 1.0 CB B:ARG287 4.7 0.1 1.0 O A:HIS283 4.8 0.2 1.0 C A:ARG287 4.9 94.4 1.0 C B:CYS285 4.9 0.9 1.0

H.B.Gristick, M.E.Rome, J.W.Chartron, M.Rao, S.Hess, S.O.Shan, W.M.Clemons. Mechanism of Assembly of A Substrate Transfer Complex During Tail-Anchored Protein Targeting. J.Biol.Chem. V. 290 30006 2015.
ISSN: ESSN 1083-351X
PubMed: 26451041
DOI: 10.1074/JBC.M115.677328