Zinc in PDB 4yzi: Crystal Structure of Blue-Shifted Channelrhodopsin Mutant (T198G/G202A)

The structure of Crystal Structure of Blue-Shifted Channelrhodopsin Mutant (T198G/G202A), PDB code: 4yzi was solved by H.E.Kato, M.Kamiya, R.Ishitani, S.Hayashi, O.Nureki, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.14 / 2.50
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	59.640, 142.420, 92.240, 90.00, 90.00, 90.00
R / Rfree (%)	22.3 / 24.1

The binding sites of Zinc atom in the Crystal Structure of Blue-Shifted Channelrhodopsin Mutant (T198G/G202A) (pdb code 4yzi). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of Blue-Shifted Channelrhodopsin Mutant (T198G/G202A), PDB code: 4yzi:

Zinc binding site 1 out of 1 in the Crystal Structure of Blue-Shifted Channelrhodopsin Mutant (T198G/G202A)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Blue-Shifted Channelrhodopsin Mutant (T198G/G202A) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn407 b:60.2 occ:1.00 OD1 A:ASP40 2.0 59.5 1.0 OD1 A:ASP142 2.1 63.2 1.0 NE2 A:HIS47 2.2 65.2 1.0 ND1 A:HIS44 2.2 64.3 1.0 CG A:ASP142 2.9 57.9 1.0 OD2 A:ASP142 3.0 62.9 1.0 CD2 A:HIS47 3.1 64.6 1.0 CG A:HIS44 3.1 67.5 1.0 CE1 A:HIS47 3.2 64.5 1.0 CE1 A:HIS44 3.2 58.6 1.0 CG A:ASP40 3.2 64.3 1.0 CB A:HIS44 3.4 64.3 1.0 CA A:HIS44 3.6 70.7 1.0 OD2 A:ASP40 3.8 63.8 1.0 CB A:ASP142 4.3 48.2 1.0 CG A:HIS47 4.3 72.3 1.0 ND1 A:HIS47 4.3 66.7 1.0 CD2 A:HIS44 4.3 65.1 1.0 NE2 A:HIS44 4.3 65.0 1.0 N A:ASP142 4.3 56.4 1.0 CB A:ASP40 4.4 58.6 1.0 C A:PHE141 4.4 53.7 1.0 N A:HIS44 4.5 62.4 1.0 O A:HIS44 4.6 66.7 1.0 C A:HIS44 4.6 70.8 1.0 CA A:ASP142 4.6 53.6 1.0 N A:PHE141 4.7 59.9 1.0 O A:PHE141 4.7 50.5 1.0 CA A:ASP40 4.8 65.7 1.0 CB A:PHE141 4.8 53.2 1.0 CA A:PHE141 4.9 58.2 1.0 O A:PHE138 4.9 56.4 1.0 CD2 A:LEU51 4.9 65.7 1.0

H.E.Kato, M.Kamiya, S.Sugo, J.Ito, R.Taniguchi, A.Orito, K.Hirata, A.Inutsuka, A.Yamanaka, A.D.Maturana, R.Ishitani, Y.Sudo, S.Hayashi, O.Nureki. Atomistic Design of Microbial Opsin-Based Blue-Shifted Optogenetics Tools. Nat Commun V. 6 7177 2015.
ISSN: ESSN 2041-1723
PubMed: 25975962
DOI: 10.1038/NCOMMS8177