Zinc in PDB 4xbh: Soluble Rabbit Neprilysin

All present enzymatic activity of Soluble Rabbit Neprilysin:
3.4.24.11;

The structure of Soluble Rabbit Neprilysin, PDB code: 4xbh was solved by S.L.Labiuk, P.Grochulski, J.Sygusch, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.60 / 2.11
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	69.361, 108.142, 212.784, 90.00, 90.00, 90.00
R / Rfree (%)	16.6 / 21.1

The binding sites of Zinc atom in the Soluble Rabbit Neprilysin (pdb code 4xbh). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Soluble Rabbit Neprilysin, PDB code: 4xbh:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Soluble Rabbit Neprilysin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Soluble Rabbit Neprilysin within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn801 b:16.6 occ:1.00 OE1 A:GLU647 2.0 11.4 1.0 NE2 A:HIS584 2.1 14.9 1.0 NE2 A:HIS588 2.2 18.0 1.0 O3 A:PO4802 2.2 24.5 1.0 O1 A:PO4802 2.6 17.8 1.0 CD A:GLU647 2.8 16.2 1.0 P A:PO4802 2.9 5.7 1.0 CD2 A:HIS588 2.9 16.1 1.0 OE2 A:GLU647 2.9 18.4 1.0 CE1 A:HIS584 3.0 14.5 1.0 CD2 A:HIS584 3.1 12.3 1.0 CE1 A:HIS588 3.3 17.8 1.0 O A:HOH1101 3.7 34.4 1.0 O4 A:PO4802 3.8 21.8 1.0 O2 A:PO4802 4.1 25.3 1.0 NE2 A:HIS712 4.1 17.1 1.0 CG A:HIS588 4.1 15.2 1.0 ND1 A:HIS584 4.2 8.8 1.0 CG A:HIS584 4.2 12.9 1.0 CG A:GLU647 4.3 12.7 1.0 O A:HOH1264 4.3 20.0 1.0 ND1 A:HIS588 4.3 18.4 1.0 O A:HOH1199 4.4 13.2 1.0 CD2 A:HIS712 4.4 13.2 1.0 OE1 A:GLU585 4.6 11.8 1.0 O A:HOH1670 4.7 39.7 1.0 OE2 A:GLU585 4.7 19.1 1.0 CB A:GLU647 4.8 11.6 1.0 CA A:GLU647 4.8 11.7 1.0 CB A:ALA650 5.0 11.5 1.0

Zinc binding site 2 out of 2 in the Soluble Rabbit Neprilysin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Soluble Rabbit Neprilysin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn801 b:22.5 occ:1.00 OE1 B:GLU647 2.0 20.0 1.0 NE2 B:HIS588 2.1 23.6 1.0 NE2 B:HIS584 2.1 20.5 1.0 O2 B:PO4802 2.2 23.6 1.0 O4 B:PO4802 2.7 24.3 1.0 CD B:GLU647 2.7 21.4 1.0 OE2 B:GLU647 2.8 22.6 1.0 P B:PO4802 2.9 11.7 1.0 CD2 B:HIS588 3.0 21.7 1.0 CE1 B:HIS584 3.0 16.6 1.0 CE1 B:HIS588 3.1 16.7 1.0 CD2 B:HIS584 3.1 16.2 1.0 O1 B:PO4802 3.7 25.1 1.0 NE2 B:HIS712 4.0 24.4 1.0 CG B:HIS588 4.1 18.0 1.0 ND1 B:HIS584 4.2 17.3 1.0 ND1 B:HIS588 4.2 16.6 1.0 CG B:GLU647 4.2 19.2 1.0 O3 B:PO4802 4.2 37.6 1.0 CG B:HIS584 4.2 18.8 1.0 CD2 B:HIS712 4.4 20.3 1.0 O B:HOH1256 4.4 33.4 1.0 O B:HOH1051 4.5 21.9 1.0 OE1 B:GLU585 4.7 21.0 1.0 OE2 B:GLU585 4.7 24.3 1.0 O B:HOH953 4.7 41.7 1.0 CB B:ALA650 4.7 19.4 1.0 CB B:GLU647 4.8 16.5 1.0 CA B:GLU647 4.8 17.2 1.0 O B:HOH1419 4.9 35.8 1.0

S.L.Labiuk, J.Sygusch, P.Grochulski. Structures of Soluble Rabbit Neprilysin Complexed with Phosphoramidon or Thiorphan. Acta Crystallogr.,Sect.F V. 75 405 2019.
ISSN: ESSN 2053-230X
PubMed: 31204686
DOI: 10.1107/S2053230X19006046