Zinc in PDB 4x8j: Crystal Structure of Murine 12F4 Fab Monoclonal Antibody Against ADAMTS5

The structure of Crystal Structure of Murine 12F4 Fab Monoclonal Antibody Against ADAMTS5, PDB code: 4x8j was solved by J.Larkin, T.A.Lohr, L.Elefante, J.Shearin, R.Matico, J.-L.Su, Y.Xue, F.Liu, C.Genell, R.E.Miller, P.B.Tran, A.-M.Malfait, C.C.Maier, C.J.Matheny, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.35
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	149.080, 149.080, 116.774, 90.00, 90.00, 90.00
R / Rfree (%)	21.7 / 23.4

The binding sites of Zinc atom in the Crystal Structure of Murine 12F4 Fab Monoclonal Antibody Against ADAMTS5 (pdb code 4x8j). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Murine 12F4 Fab Monoclonal Antibody Against ADAMTS5, PDB code: 4x8j:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Murine 12F4 Fab Monoclonal Antibody Against ADAMTS5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Murine 12F4 Fab Monoclonal Antibody Against ADAMTS5 within 5.0Å range: probe atom residue distance (Å) B Occ L:Zn301 b:53.4 occ:1.00 O13 H:2PE301 2.8 42.1 1.0 O16 H:2PE301 3.0 41.7 1.0 O H:HOH425 3.0 54.5 1.0 O H:HOH401 3.1 57.1 1.0 C9 H:2PE301 3.1 45.8 1.0 O H:HOH409 3.2 40.4 1.0 O H:HOH426 3.4 52.7 1.0 C20 H:2PE301 3.4 44.0 1.0 O19 H:2PE301 3.5 43.2 1.0 C15 H:2PE301 3.5 41.6 1.0 O10 H:2PE301 3.5 44.0 1.0 C18 H:2PE301 3.6 42.5 1.0 C14 H:2PE301 3.7 41.8 1.0 C12 H:2PE301 3.8 41.8 1.0 C17 H:2PE301 3.8 42.4 1.0 C11 H:2PE301 3.9 42.9 1.0 C8 H:2PE301 4.1 47.8 1.0 O7 H:2PE301 4.5 51.2 1.0 C21 H:2PE301 4.6 43.8 1.0 O1 H:2PE301 4.9 57.9 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Murine 12F4 Fab Monoclonal Antibody Against ADAMTS5


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Murine 12F4 Fab Monoclonal Antibody Against ADAMTS5 within 5.0Å range: probe atom residue distance (Å) B Occ H:Zn303 b:66.6 occ:1.00 O H:ASP31 2.5 28.2 1.0 O19 H:2PE302 2.8 41.4 1.0 O1 H:2PE302 2.9 65.2 1.0 O16 H:2PE302 2.9 41.2 1.0 O13 H:2PE302 3.0 42.4 1.0 O H:HOH478 3.1 43.7 1.0 O H:HOH479 3.4 49.4 1.0 C17 H:2PE302 3.6 41.4 1.0 C21 H:2PE302 3.6 41.8 1.0 C23 H:2PE302 3.6 43.3 1.0 C15 H:2PE302 3.6 40.8 1.0 C18 H:2PE302 3.7 40.9 1.0 C H:ASP31 3.7 28.2 1.0 O22 H:2PE302 3.7 43.0 1.0 C12 H:2PE302 3.8 45.5 1.0 C2 H:2PE302 3.8 65.6 1.0 C20 H:2PE302 3.8 42.1 1.0 C14 H:2PE302 3.8 41.5 1.0 C11 H:2PE302 3.9 47.5 1.0 C3 H:2PE302 4.0 65.4 1.0 O10 H:2PE302 4.2 51.6 1.0 CA H:ASP31 4.4 28.0 1.0 CB H:ASP31 4.4 28.3 1.0 O4 H:2PE302 4.5 64.2 1.0 O H:HOH427 4.6 30.3 1.0 C24 H:2PE302 4.7 42.9 1.0 N H:ALA32 4.8 28.7 1.0 CA H:ALA32 5.0 29.0 1.0

J.Larkin, T.A.Lohr, L.Elefante, J.Shearin, R.Matico, J.L.Su, Y.Xue, F.Liu, C.Genell, R.E.Miller, P.B.Tran, A.M.Malfait, C.C.Maier, C.J.Matheny. Translational Development of An Adamts-5 Antibody For Osteoarthritis Disease Modification. Osteoarthr. Cartil. 2015.
ISSN: ISSN 1522-9653
PubMed: 25800415
DOI: 10.1016/J.JOCA.2015.02.778