Zinc in PDB 4u7d: Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide

All present enzymatic activity of Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide:
3.6.4.12;

The structure of Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide, PDB code: 4u7d was solved by A.C.W.Pike, Y.Zhang, C.Schnecke, C.D.O.Cooper, F.Von Delft, C.H.Arrowsmith, A.Edwards, C.Bountra, O.Gileadi, Structural Genomicsconsortium (Sgc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.90 / 3.40
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.380, 138.220, 207.581, 90.00, 90.02, 90.00
R / Rfree (%)	20.5 / 24.9

The binding sites of Zinc atom in the Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide (pdb code 4u7d). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide, PDB code: 4u7d:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn701 b:75.1 occ:1.00 SG A:CYS453 2.3 80.0 1.0 SG A:CYS471 2.3 79.9 1.0 SG A:CYS475 2.3 76.0 1.0 SG A:CYS478 2.3 81.6 1.0 CB A:CYS471 2.9 84.5 1.0 CB A:CYS475 3.2 73.7 1.0 CB A:CYS478 3.3 82.6 1.0 CB A:CYS453 3.4 79.0 1.0 CA A:CYS453 3.8 74.5 1.0 N A:ARG454 3.9 74.3 1.0 N A:CYS475 4.0 79.7 1.0 CA A:CYS475 4.1 75.3 1.0 C A:CYS453 4.2 70.7 1.0 CA A:CYS471 4.4 88.6 1.0 CA A:CYS478 4.4 81.5 1.0 N A:CYS478 4.4 76.3 1.0 C A:MET474 4.5 79.4 1.0 N A:MET474 4.6 87.7 1.0 N A:ARG455 4.6 75.5 1.0 O A:CYS475 4.6 79.1 1.0 C A:CYS475 4.8 75.6 1.0 O A:MET474 4.9 76.6 1.0 CA A:ARG454 4.9 70.9 1.0 CB A:ARG455 5.0 81.6 1.0

Zinc binding site 2 out of 4 in the Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn701 b:91.6 occ:1.00 SG B:CYS475 2.3 88.0 1.0 SG B:CYS478 2.3 95.5 1.0 SG B:CYS471 2.3 95.6 1.0 SG B:CYS453 2.3 90.2 1.0 CB B:CYS478 2.9 98.0 1.0 CB B:CYS471 3.1 0.2 1.0 CB B:CYS475 3.4 85.8 1.0 CB B:CYS453 3.5 89.6 1.0 N B:CYS475 3.9 94.0 1.0 CA B:CYS453 4.0 85.1 1.0 CA B:CYS478 4.1 97.4 1.0 N B:CYS478 4.1 91.6 1.0 CA B:CYS475 4.1 89.0 1.0 N B:ARG454 4.3 84.4 1.0 O B:CYS475 4.3 94.6 1.0 N B:MET474 4.4 99.8 1.0 C B:MET474 4.5 90.2 1.0 C B:CYS453 4.6 80.3 1.0 C B:CYS475 4.6 90.3 1.0 CA B:CYS471 4.6 0.9 1.0

Zinc binding site 3 out of 4 in the Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn701 b:94.6 occ:1.00 SG C:CYS471 2.3 0.7 1.0 SG C:CYS478 2.3 0.8 1.0 SG C:CYS475 2.3 95.1 1.0 SG C:CYS453 2.3 97.7 1.0 CB C:CYS471 3.1 1.0 1.0 CB C:CYS478 3.1 0.6 1.0 CB C:CYS475 3.4 93.0 1.0 CB C:CYS453 3.5 97.3 1.0 N C:CYS475 3.9 99.6 1.0 CA C:CYS453 4.0 94.2 1.0 CA C:CYS475 4.2 96.0 1.0 N C:ARG454 4.2 93.7 1.0 CA C:CYS478 4.3 0.2 1.0 N C:CYS478 4.3 99.8 1.0 N C:MET474 4.4 0.0 1.0 C C:MET474 4.4 0.2 1.0 C C:CYS453 4.5 89.8 1.0 CA C:CYS471 4.5 0.6 1.0 O C:CYS475 4.6 0.8 1.0 C C:CYS475 4.8 98.0 1.0 N C:ARG455 4.8 90.8 1.0 O C:MET474 5.0 97.1 1.0

Zinc binding site 4 out of 4 in the Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn701 b:0.8 occ:1.00 SG D:CYS471 2.3 0.9 1.0 SG D:CYS475 2.3 0.7 1.0 SG D:CYS453 2.3 0.7 1.0 SG D:CYS478 2.3 0.8 1.0 CB D:CYS475 3.1 0.2 1.0 CB D:CYS471 3.1 0.6 1.0 CB D:CYS478 3.3 0.1 1.0 CB D:CYS453 3.7 0.6 1.0 N D:CYS475 3.8 0.7 1.0 N D:ARG454 3.9 0.3 1.0 CA D:CYS453 4.0 0.8 1.0 CA D:CYS475 4.0 0.1 1.0 C D:MET474 4.3 0.3 1.0 C D:CYS453 4.3 0.3 1.0 N D:ARG455 4.4 0.5 1.0 CA D:CYS478 4.5 1.0 1.0 N D:MET474 4.5 0.1 1.0 N D:CYS478 4.5 0.9 1.0 CA D:CYS471 4.6 0.5 1.0 O D:CYS475 4.7 0.1 1.0 C D:CYS475 4.7 0.4 1.0 O D:MET474 4.7 0.9 1.0 CB D:ARG455 4.8 0.5 1.0 CG D:ARG455 4.8 0.4 1.0 NH2 D:ARG455 4.8 0.9 1.0 CD D:ARG455 4.9 0.2 1.0 CA D:ARG454 4.9 0.4 1.0 CB D:ARG454 4.9 0.2 1.0 CA D:MET474 5.0 0.8 1.0

A.C.W.Pike, Y.Zhang, C.Schnecke, C.D.O.Cooper, F.Von Delft, C.H.Arrowsmith, A.Edwards, C.Bountra, O.Gileadi. Structure of Human Recq-Like Helicase in Complex with An Oligonucleotide To Be Published.