Zinc in PDB 4k4t: Poliovirus Polymerase Elongation Complex (R4_FORM)

All present enzymatic activity of Poliovirus Polymerase Elongation Complex (R4_FORM):
2.7.7.48;

The structure of Poliovirus Polymerase Elongation Complex (R4_FORM), PDB code: 4k4t was solved by P.Gong, O.B.Peersen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.96 / 2.75
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	59.401, 59.248, 97.455, 78.11, 78.09, 78.85
R / Rfree (%)	20.2 / 26.2

The binding sites of Zinc atom in the Poliovirus Polymerase Elongation Complex (R4_FORM) (pdb code 4k4t). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Poliovirus Polymerase Elongation Complex (R4_FORM), PDB code: 4k4t:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Poliovirus Polymerase Elongation Complex (R4_FORM)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Poliovirus Polymerase Elongation Complex (R4_FORM) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn502 b:43.6 occ:1.00 NE2 A:HIS272 2.0 53.0 1.0 NE2 A:HIS270 2.0 55.9 1.0 SG A:CYS281 2.1 61.2 1.0 CE1 A:HIS272 2.9 61.5 1.0 CE1 A:HIS270 2.9 55.3 1.0 CD2 A:HIS272 3.0 55.9 1.0 CD2 A:HIS270 3.1 55.2 1.0 CB A:CYS281 3.4 60.4 1.0 ND1 A:HIS272 4.0 64.2 1.0 CG A:HIS272 4.1 53.2 1.0 ND1 A:HIS270 4.1 50.4 1.0 CG A:HIS270 4.2 52.3 1.0 NE1 A:TRP5 4.3 63.1 1.0 CA A:CYS281 4.5 55.4 1.0 CZ2 A:TRP5 4.6 60.4 1.0 CE2 A:TRP5 4.8 61.4 1.0 CE A:LYS283 4.8 52.5 1.0

Zinc binding site 2 out of 2 in the Poliovirus Polymerase Elongation Complex (R4_FORM)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Poliovirus Polymerase Elongation Complex (R4_FORM) within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn503 b:40.2 occ:1.00 NE2 E:HIS270 2.0 59.8 1.0 NE2 E:HIS272 2.0 53.5 1.0 SG E:CYS281 2.2 61.0 1.0 CE1 E:HIS270 2.9 55.2 1.0 CD2 E:HIS272 3.0 55.2 1.0 CD2 E:HIS270 3.0 58.0 1.0 CE1 E:HIS272 3.0 60.0 1.0 CB E:CYS281 3.5 60.5 1.0 ND1 E:HIS270 4.0 47.0 1.0 CG E:HIS272 4.1 55.1 1.0 ND1 E:HIS272 4.1 59.3 1.0 CG E:HIS270 4.1 46.6 1.0 NE1 E:TRP5 4.4 55.2 1.0 CA E:CYS281 4.6 58.4 1.0 CZ2 E:TRP5 4.6 60.3 1.0 CE2 E:TRP5 4.8 58.5 1.0 CE E:LYS283 4.8 58.7 1.0

P.Gong, M.G.Kortus, J.C.Nix, R.E.Davis, O.B.Peersen. Structures of Coxsackievirus, Rhinovirus, and Poliovirus Polymerase Elongation Complexes Solved By Engineering Rna Mediated Crystal Contacts. Plos One V. 8 60272 2013.
ISSN: ESSN 1932-6203
PubMed: 23667424
DOI: 10.1371/JOURNAL.PONE.0060272