Zinc in PDB 4cth: Neprilysin Variant G399V,G714K in Complex with Phosphoramidon

All present enzymatic activity of Neprilysin Variant G399V,G714K in Complex with Phosphoramidon:
3.4.24.11;

The structure of Neprilysin Variant G399V,G714K in Complex with Phosphoramidon, PDB code: 4cth was solved by C.I.Webster, M.Burrell, L.Olsson, S.B.Fowler, S.Digby, A.Sandercock, A.Snijder, J.Tebbe, U.Haupts, J.Grudzinska, L.Jermutus, C.Andersson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.48 / 2.15
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	108.799, 108.799, 112.942, 90.00, 90.00, 120.00
R / Rfree (%)	20.95 / 26.14

The binding sites of Zinc atom in the Neprilysin Variant G399V,G714K in Complex with Phosphoramidon (pdb code 4cth). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Neprilysin Variant G399V,G714K in Complex with Phosphoramidon, PDB code: 4cth:

Zinc binding site 1 out of 1 in the Neprilysin Variant G399V,G714K in Complex with Phosphoramidon


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Neprilysin Variant G399V,G714K in Complex with Phosphoramidon within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1750 b:27.0 occ:1.00 OE1 A:GLU646 1.9 27.2 1.0 O2P A:RDF1759 2.0 31.0 1.0 NE2 A:HIS587 2.0 33.0 1.0 NE2 A:HIS583 2.0 25.6 1.0 CD A:GLU646 2.7 26.0 1.0 OE2 A:GLU646 2.7 26.3 1.0 CE1 A:HIS583 2.8 25.1 1.0 CE1 A:HIS587 2.9 32.8 1.0 CD2 A:HIS587 3.0 33.2 1.0 P A:RDF1759 3.0 29.1 1.0 CD2 A:HIS583 3.1 25.7 1.0 O1P A:RDF1759 3.1 28.1 1.0 NE2 A:HIS711 4.0 29.8 1.0 ND1 A:HIS583 4.0 25.6 1.0 N A:RDF1759 4.1 25.9 1.0 CA A:RDF1759 4.1 27.8 1.0 ND1 A:HIS587 4.1 33.1 1.0 CG A:HIS587 4.1 31.0 1.0 CG A:GLU646 4.1 26.0 1.0 CG A:HIS583 4.2 25.1 1.0 O1 A:RDF1759 4.2 33.3 1.0 CD2 A:HIS711 4.3 30.6 1.0 O A:RDF1759 4.3 26.9 1.0 C A:RDF1759 4.4 26.9 1.0 CB A:ALA649 4.6 27.9 1.0 CB A:GLU646 4.7 23.8 1.0 CA A:GLU646 4.7 22.5 1.0 O5 A:RDF1759 4.7 33.6 1.0 NH2 A:ARG717 4.8 21.8 1.0 C1 A:RDF1759 4.9 33.6 1.0 O3 A:GOL1751 4.9 47.7 1.0 OE2 A:GLU584 4.9 38.6 1.0

C.I.Webster, M.Burrell, L.Olsson, S.B.Fowler, S.Digby, A.Sandercock, A.Snijder, J.Tebbe, U.Haupts, J.Grudzinska, L.Jermutus, C.Andersson. Engineering Neprilysin Activity and Specificity to Create A Novel Therapeutic For Alzheimer'S Disease. Plos One V. 9 04001 2014.
ISSN: ISSN 1932-6203
PubMed: 25089527
DOI: 10.1371/JOURNAL.PONE.0104001