Zinc in PDB 4csa: Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer

The structure of Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer, PDB code: 4csa was solved by C.Leyrat, M.Renner, K.Harlos, J.M.Grimes, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.67 / 2.28
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.140, 93.900, 85.510, 90.00, 95.82, 90.00
R / Rfree (%)	19.35 / 22.21

The binding sites of Zinc atom in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer (pdb code 4csa). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer, PDB code: 4csa:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1171 b:50.5 occ:0.76 NE2 A:HIS25 2.0 62.0 1.0 SG A:CYS15 2.2 66.7 1.0 SG A:CYS21 2.3 86.5 1.0 SG A:CYS7 2.3 52.4 1.0 CE1 A:HIS25 2.8 62.3 1.0 CD2 A:HIS25 3.1 62.3 1.0 CB A:CYS15 3.1 63.7 1.0 CB A:CYS7 3.3 49.4 1.0 CB A:CYS21 3.4 83.7 1.0 ND1 A:HIS25 4.0 63.6 1.0 CG A:HIS25 4.1 61.6 1.0 CA A:CYS15 4.5 64.4 1.0 CD2 A:TYR9 4.6 54.0 1.0 CA A:CYS7 4.7 49.7 1.0 CA A:CYS21 4.8 84.3 1.0 CB A:TYR9 4.8 51.1 1.0 CB A:PHE23 4.9 73.6 1.0 O A:PHE23 5.0 71.2 1.0

Zinc binding site 2 out of 4 in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1168 b:62.0 occ:0.77 NE2 B:HIS25 2.0 68.6 1.0 SG B:CYS21 2.2 88.9 1.0 SG B:CYS15 2.2 70.5 1.0 SG B:CYS7 2.3 72.5 1.0 CE1 B:HIS25 2.9 68.7 1.0 CB B:CYS21 3.0 85.6 1.0 CD2 B:HIS25 3.1 68.5 1.0 CB B:CYS15 3.2 66.9 1.0 CB B:CYS7 3.3 69.2 1.0 ND1 B:HIS25 4.1 69.2 1.0 CG B:HIS25 4.2 66.8 1.0 CA B:CYS21 4.5 86.2 1.0 CA B:CYS15 4.5 67.3 1.0 CA B:CYS7 4.6 69.4 1.0 CD2 B:TYR9 4.6 68.6 1.0 CB B:TYR9 4.8 64.2 1.0 CB B:PHE23 4.9 83.2 1.0 CD B:ARG17 4.9 0.3 1.0 C B:CYS21 5.0 89.9 1.0

Zinc binding site 3 out of 4 in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1174 b:54.0 occ:0.72 NE2 C:HIS25 2.0 61.1 1.0 SG C:CYS15 2.2 77.6 1.0 SG C:CYS21 2.3 84.3 1.0 SG C:CYS7 2.3 62.2 1.0 CE1 C:HIS25 2.9 61.3 1.0 CB C:CYS15 3.1 73.1 1.0 CD2 C:HIS25 3.1 61.2 1.0 CB C:CYS21 3.2 81.2 1.0 CB C:CYS7 3.3 58.0 1.0 O C:HOH2005 4.0 66.2 1.0 ND1 C:HIS25 4.1 61.8 1.0 CG C:HIS25 4.2 59.9 1.0 O2 G:DT3 4.4 0.8 1.0 CA C:CYS15 4.5 72.9 1.0 CB C:PHE23 4.6 73.9 1.0 CA C:CYS7 4.6 57.8 1.0 CA C:CYS21 4.7 82.3 1.0 N3 G:DT3 5.0 0.6 1.0 CB C:ARG17 5.0 91.4 1.0

Zinc binding site 4 out of 4 in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer Bound to A Dna 4-Mer within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn1177 b:44.1 occ:0.83 NE2 E:HIS25 2.0 50.3 1.0 SG E:CYS15 2.2 51.8 1.0 SG E:CYS21 2.3 51.7 1.0 SG E:CYS7 2.3 58.0 1.0 CE1 E:HIS25 2.9 50.2 1.0 CD2 E:HIS25 3.1 50.4 1.0 CB E:CYS15 3.2 48.5 1.0 CB E:CYS21 3.2 48.6 1.0 CB E:CYS7 3.3 54.9 1.0 O E:HOH2008 3.9 50.6 1.0 ND1 E:HIS25 4.0 50.9 1.0 CG E:HIS25 4.1 49.5 1.0 CA E:CYS15 4.5 49.1 1.0 CA E:CYS7 4.6 55.7 1.0 CA E:CYS21 4.7 48.6 1.0 CB E:PHE23 4.8 54.7 1.0 CD2 E:TYR9 4.8 58.9 1.0 CB E:TYR9 4.9 54.3 1.0 CB E:ARG17 4.9 47.3 1.0

C.Leyrat, M.Renner, K.Harlos, J.T.Huiskonen, J.M.Grimes. Drastic Changes in Conformational Dynamics of the Antiterminator M2-1 Regulate Transcription Efficiency in Pneumovirinae. Elife V. 3 02674 2014.
ISSN: ISSN 2050-084X
PubMed: 24842877
DOI: 10.7554/ELIFE.02674