Zinc in PDB 4cs7: Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1

The structure of Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1, PDB code: 4cs7 was solved by C.Leyrat, M.Renner, K.Harlos, J.M.Grimes, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.13 / 2.47
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.150, 92.670, 82.830, 90.00, 94.48, 90.00
R / Rfree (%)	23.34 / 26.15

The binding sites of Zinc atom in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1 (pdb code 4cs7). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1, PDB code: 4cs7:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1170 b:0.1 occ:1.00 NE2 A:HIS25 1.9 0.8 1.0 SG A:CYS15 2.2 0.0 1.0 SG A:CYS21 2.5 0.4 1.0 SG A:CYS7 2.5 0.8 1.0 CE1 A:HIS25 2.8 0.1 1.0 CD2 A:HIS25 2.9 0.4 1.0 CB A:CYS15 3.0 0.1 1.0 CB A:CYS21 3.3 0.7 1.0 CB A:CYS7 3.5 0.3 1.0 ND1 A:HIS25 3.9 0.5 1.0 CG A:HIS25 4.0 0.3 1.0 CA A:CYS15 4.4 0.6 1.0 CA A:CYS21 4.7 0.9 1.0 CG A:GLU10 4.8 99.9 1.0 CB A:TYR9 4.9 88.3 1.0 CA A:CYS7 4.9 0.1 1.0 CD2 A:TYR9 5.0 90.3 1.0

Zinc binding site 2 out of 4 in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1170 b:93.3 occ:1.00 SG B:CYS21 2.2 89.4 1.0 SG B:CYS7 2.2 83.6 1.0 NE2 B:HIS25 2.2 77.6 1.0 SG B:CYS15 2.4 85.8 1.0 CE1 B:HIS25 3.1 77.5 1.0 CB B:CYS21 3.2 86.8 1.0 CB B:CYS7 3.2 79.9 1.0 CB B:CYS15 3.3 80.0 1.0 CD2 B:HIS25 3.3 76.9 1.0 O A:HOH2009 4.2 54.7 1.0 ND1 B:HIS25 4.2 78.2 1.0 CD1 B:TYR9 4.3 75.8 1.0 CG B:HIS25 4.3 75.3 1.0 CB B:PHE23 4.4 88.7 1.0 CA B:CYS7 4.5 80.8 1.0 NH1 B:ARG17 4.5 0.8 1.0 CA B:CYS21 4.6 89.3 1.0 CA B:CYS15 4.7 78.6 1.0 CD2 B:PHE23 4.7 96.1 1.0 CB B:TYR9 4.8 71.8 1.0 N B:GLU10 4.9 65.5 1.0 CG B:TYR9 4.9 74.0 1.0 CD B:ARG17 4.9 0.0 1.0 N B:PHE23 5.0 88.1 1.0

Zinc binding site 3 out of 4 in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn1170 b:0.9 occ:1.00 NE2 C:HIS25 1.9 0.7 1.0 SG C:CYS21 2.2 0.1 1.0 SG C:CYS15 2.7 0.9 1.0 CD2 C:HIS25 2.8 0.7 1.0 SG C:CYS7 3.0 0.5 1.0 CE1 C:HIS25 3.0 0.6 1.0 CB C:CYS7 3.5 0.2 1.0 CB C:CYS21 3.7 0.4 1.0 CG C:HIS25 3.9 0.8 1.0 ND1 C:HIS25 4.0 0.3 1.0 CB C:CYS15 4.1 0.6 1.0 CB C:TYR9 4.3 0.6 1.0 O C:TYR9 4.4 0.6 1.0 O C:CYS21 4.6 0.5 1.0 CA C:CYS21 4.8 0.5 1.0 N C:CYS21 4.9 0.3 1.0 CA C:CYS7 4.9 0.7 1.0 C C:TYR9 4.9 0.7 1.0

Zinc binding site 4 out of 4 in the Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of the Asymmetric Human Metapneumovirus M2-1 Tetramer, Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn1170 b:71.7 occ:1.00 NE2 E:HIS25 2.1 53.0 1.0 SG E:CYS7 2.2 54.2 1.0 SG E:CYS15 2.3 52.6 1.0 SG E:CYS21 2.4 52.8 1.0 CE1 E:HIS25 3.0 51.3 1.0 CD2 E:HIS25 3.1 51.6 1.0 CB E:CYS15 3.2 48.2 1.0 CB E:CYS7 3.3 50.1 1.0 CB E:CYS21 3.4 48.4 1.0 ND1 E:HIS25 4.1 51.1 1.0 CG E:HIS25 4.2 49.3 1.0 CA E:CYS15 4.5 49.7 1.0 CA E:CYS7 4.6 51.8 1.0 CB E:PHE23 4.8 58.6 1.0 CA E:CYS21 4.8 49.4 1.0 CD2 E:TYR9 4.8 72.1 1.0 CB E:TYR9 4.9 58.5 1.0 CB E:ARG17 4.9 52.3 1.0

C.Leyrat, M.Renner, K.Harlos, J.T.Huiskonen, J.M.Grimes. Drastic Changes in Conformational Dynamics of the Antiterminator M2-1 Regulate Transcription Efficiency in Pneumovirinae. Elife V. 3 02674 2014.
ISSN: ISSN 2050-084X
PubMed: 24842877
DOI: 10.7554/ELIFE.02674