Zinc in PDB 3vv2: Crystal Structure of Complex Form Between S324A-Subtilisin and Mutant Tkpro

The structure of Crystal Structure of Complex Form Between S324A-Subtilisin and Mutant Tkpro, PDB code: 3vv2 was solved by R.Uehara, Y.Ueda, D.J.You, K.Takano, Y.Koga, S.Kanaya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.12 / 1.83
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	65.051, 68.583, 74.054, 90.00, 90.00, 90.00
R / Rfree (%)	21.8 / 26.8

The structure of Crystal Structure of Complex Form Between S324A-Subtilisin and Mutant Tkpro also contains other interesting chemical elements:

Chlorine	(Cl)	1 atom
Calcium	(Ca)	7 atoms

The binding sites of Zinc atom in the Crystal Structure of Complex Form Between S324A-Subtilisin and Mutant Tkpro (pdb code 3vv2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Crystal Structure of Complex Form Between S324A-Subtilisin and Mutant Tkpro, PDB code: 3vv2:

Zinc binding site 1 out of 1 in the Crystal Structure of Complex Form Between S324A-Subtilisin and Mutant Tkpro


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Complex Form Between S324A-Subtilisin and Mutant Tkpro within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2001 b:66.7 occ:1.00 OD2 B:ASP42 2.2 34.6 1.0 CD2 B:HIS28 3.0 49.3 1.0 NE2 B:HIS28 3.0 51.3 1.0 CG B:ASP42 3.2 33.2 1.0 CB B:ASP42 3.5 32.4 1.0 NH2 B:ARG7 4.2 25.6 1.0 OD1 B:ASP42 4.3 28.5 1.0 CE1 B:HIS28 4.3 51.0 1.0 CG B:HIS28 4.3 43.5 1.0 OG1 B:THR5 4.8 41.4 1.0 CG2 B:VAL30 4.9 28.9 1.0 ND1 B:HIS28 5.0 49.0 1.0

R.Uehara, Y.Ueda, D.J.You, Y.Koga, S.Kanaya. Accelerated Maturation of Tk-Subtilisin By A Leu Pro Mutation at the C-Terminus of the Propeptide, Which Reduces the Binding of the Propeptide to Tk-Subtilisin Febs J. V. 280 994 2013.
ISSN: ISSN 1742-464X
PubMed: 23237738
DOI: 10.1111/FEBS.12091