Zinc in PDB 3ts2: Mouse LIN28A in Complex with Let-7G Microrna Pre-Element

The structure of Mouse LIN28A in Complex with Let-7G Microrna Pre-Element, PDB code: 3ts2 was solved by Y.Nam, P.Sliz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.82 / 2.01
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	46.030, 109.210, 182.910, 90.00, 90.00, 90.00
R / Rfree (%)	19 / 21.7

The binding sites of Zinc atom in the Mouse LIN28A in Complex with Let-7G Microrna Pre-Element (pdb code 3ts2). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Mouse LIN28A in Complex with Let-7G Microrna Pre-Element, PDB code: 3ts2:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Mouse LIN28A in Complex with Let-7G Microrna Pre-Element


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mouse LIN28A in Complex with Let-7G Microrna Pre-Element within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1 b:40.6 occ:1.00 NE2 A:HIS147 2.1 50.5 1.0 SG A:CYS142 2.3 35.7 1.0 SG A:CYS139 2.3 49.5 1.0 SG A:CYS152 2.3 39.5 1.0 CD2 A:HIS147 3.1 52.8 1.0 CE1 A:HIS147 3.1 49.7 1.0 CB A:CYS152 3.1 39.5 1.0 CB A:CYS139 3.1 46.2 1.0 CB A:CYS142 3.1 32.4 1.0 N A:CYS142 3.7 36.1 1.0 CA A:CYS152 3.9 42.0 1.0 CA A:CYS142 3.9 34.0 1.0 O A:HOH206 4.0 38.3 1.0 CG A:HIS147 4.2 52.5 1.0 ND1 A:HIS147 4.2 52.3 1.0 O A:HOH290 4.4 42.2 1.0 CB A:ASN141 4.5 37.0 1.0 N A:GLY143 4.5 39.6 1.0 CA A:CYS139 4.6 47.4 1.0 C A:CYS142 4.6 39.6 1.0 N A:GLY144 4.7 38.1 1.0 C A:ASN141 4.7 40.4 1.0 N A:CYS152 4.8 46.3 1.0 N A:ASN141 4.9 40.5 1.0 CA A:ASN141 4.9 39.4 1.0 C A:CYS152 4.9 48.1 1.0

Zinc binding site 2 out of 4 in the Mouse LIN28A in Complex with Let-7G Microrna Pre-Element


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mouse LIN28A in Complex with Let-7G Microrna Pre-Element within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn2 b:35.3 occ:1.00 NE2 A:HIS169 2.0 38.4 1.0 SG A:CYS164 2.3 31.6 1.0 SG A:CYS161 2.3 35.7 1.0 SG A:CYS174 2.3 41.1 1.0 CD2 A:HIS169 3.0 40.2 1.0 CE1 A:HIS169 3.1 37.7 1.0 CB A:CYS174 3.1 40.4 1.0 CB A:CYS161 3.2 30.8 1.0 CB A:CYS164 3.4 28.5 1.0 N A:CYS164 3.7 28.9 1.0 CA A:CYS174 3.9 42.5 1.0 O A:HOH283 4.1 50.2 1.0 CA A:CYS164 4.1 29.1 1.0 CG A:HIS169 4.2 39.8 1.0 ND1 A:HIS169 4.2 39.1 1.0 CB A:SER166 4.6 33.9 1.0 CD A:PRO175 4.6 49.2 1.0 CB A:PHE163 4.6 36.6 1.0 CA A:CYS161 4.6 32.6 1.0 C A:CYS174 4.8 48.2 1.0 C A:PHE163 4.8 34.8 1.0 C A:CYS164 4.8 33.6 1.0 N A:SER166 4.9 28.9 1.0 N A:CYS174 4.9 46.3 1.0 N A:GLN165 4.9 29.2 1.0 N A:PRO175 5.0 48.4 1.0

Zinc binding site 3 out of 4 in the Mouse LIN28A in Complex with Let-7G Microrna Pre-Element


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Mouse LIN28A in Complex with Let-7G Microrna Pre-Element within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn1 b:42.2 occ:1.00 NE2 B:HIS147 2.1 49.4 1.0 SG B:CYS142 2.2 39.8 1.0 SG B:CYS152 2.3 43.5 1.0 SG B:CYS139 2.4 40.3 1.0 CE1 B:HIS147 3.1 48.5 1.0 CD2 B:HIS147 3.1 51.3 1.0 CB B:CYS139 3.1 37.4 1.0 CB B:CYS152 3.2 41.6 1.0 CB B:CYS142 3.2 36.0 1.0 N B:CYS142 3.6 39.6 1.0 CA B:CYS152 4.0 43.1 1.0 CA B:CYS142 4.0 37.1 1.0 ND1 B:HIS147 4.2 51.2 1.0 CG B:HIS147 4.2 51.4 1.0 CB B:ASN141 4.5 41.1 1.0 O B:HOH220 4.6 44.3 1.0 CA B:CYS139 4.6 39.3 1.0 C B:ASN141 4.6 42.5 1.0 C B:CYS142 4.8 40.2 1.0 N B:GLY143 4.8 39.1 1.0 N B:GLY144 4.8 40.1 1.0 N B:CYS152 4.9 46.3 1.0 ND2 B:ASN141 4.9 37.0 1.0 CA B:ASN141 4.9 41.3 1.0 C B:CYS152 5.0 45.8 1.0

Zinc binding site 4 out of 4 in the Mouse LIN28A in Complex with Let-7G Microrna Pre-Element


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Mouse LIN28A in Complex with Let-7G Microrna Pre-Element within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn2 b:41.2 occ:1.00 NE2 B:HIS169 2.2 50.2 1.0 SG B:CYS161 2.3 38.3 1.0 SG B:CYS174 2.3 43.4 1.0 SG B:CYS164 2.4 37.2 1.0 CB B:CYS174 3.1 42.7 1.0 CD2 B:HIS169 3.1 52.0 1.0 CB B:CYS161 3.2 35.4 1.0 CE1 B:HIS169 3.2 50.0 1.0 CB B:CYS164 3.3 33.6 1.0 N B:CYS164 3.6 34.5 1.0 CA B:CYS174 3.9 46.0 1.0 CA B:CYS164 4.0 33.2 1.0 O B:HOH214 4.1 50.7 1.0 CG B:HIS169 4.3 52.2 1.0 ND1 B:HIS169 4.4 52.4 1.0 CB B:PHE163 4.5 40.4 1.0 C B:PHE163 4.6 39.8 1.0 CA B:CYS161 4.6 37.3 1.0 CB B:SER166 4.6 48.4 1.0 CD B:PRO175 4.7 55.1 1.0 C B:CYS164 4.7 37.7 1.0 C B:CYS174 4.8 53.3 1.0 N B:GLN165 4.8 35.8 1.0 CA B:PHE163 4.9 37.7 1.0 N B:CYS174 5.0 49.9 1.0 N B:PHE163 5.0 40.3 1.0 N B:SER166 5.0 40.7 1.0

Y.Nam, C.Chen, R.I.Gregory, J.J.Chou, P.Sliz. Molecular Basis For Interaction of Let-7 Micrornas with LIN28. Cell(Cambridge,Mass.) V. 147 1080 2011.
ISSN: ISSN 0092-8674
PubMed: 22078496
DOI: 10.1016/J.CELL.2011.10.020