Zinc in PDB 3mia: Crystal Structure of Hiv-1 Tat Complexed with Atp-Bound Human P-Tefb

All present enzymatic activity of Crystal Structure of Hiv-1 Tat Complexed with Atp-Bound Human P-Tefb:
2.7.11.22; 2.7.11.23;

The structure of Crystal Structure of Hiv-1 Tat Complexed with Atp-Bound Human P-Tefb, PDB code: 3mia was solved by T.H.Tahirov, N.D.Babayeva, K.Varzavand, J.J.Cooper, S.C.Sedore, D.H.Price, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.61 / 3.00
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	134.286, 134.286, 97.185, 90.00, 90.00, 120.00
R / Rfree (%)	21.9 / 28.7

The structure of Crystal Structure of Hiv-1 Tat Complexed with Atp-Bound Human P-Tefb also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the Crystal Structure of Hiv-1 Tat Complexed with Atp-Bound Human P-Tefb (pdb code 3mia). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Hiv-1 Tat Complexed with Atp-Bound Human P-Tefb, PDB code: 3mia:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Hiv-1 Tat Complexed with Atp-Bound Human P-Tefb


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Hiv-1 Tat Complexed with Atp-Bound Human P-Tefb within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn87 b:82.1 occ:1.00 ND1 C:HIS33 2.3 79.8 1.0 SG C:CYS37 2.3 76.3 1.0 SG C:CYS34 2.3 82.8 1.0 SG C:CYS22 2.3 81.9 1.0 CG C:HIS33 3.1 79.8 1.0 CB C:HIS33 3.2 81.0 1.0 CE1 C:HIS33 3.3 79.4 1.0 CB C:CYS37 3.4 71.8 1.0 CB C:CYS34 3.5 79.7 1.0 CB C:CYS22 3.6 83.1 1.0 N C:CYS34 3.6 80.9 1.0 CA C:CYS22 4.0 82.4 1.0 CA C:CYS34 4.2 79.7 1.0 N C:CYS37 4.2 71.0 1.0 C C:HIS33 4.3 81.7 1.0 CD2 C:HIS33 4.3 79.2 1.0 CA C:CYS37 4.4 71.0 1.0 CA C:HIS33 4.4 81.5 1.0 NE2 C:HIS33 4.4 79.2 1.0 N C:CYS22 4.8 80.9 1.0 CG C:PRO18 4.9 67.4 1.0 C C:CYS34 4.9 78.5 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Hiv-1 Tat Complexed with Atp-Bound Human P-Tefb


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Hiv-1 Tat Complexed with Atp-Bound Human P-Tefb within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn88 b:93.9 occ:1.00 SG C:CYS27 2.3 91.3 1.0 SG B:CYS261 2.3 98.9 1.0 SG C:CYS30 2.3 96.2 1.0 SG C:CYS25 2.3 86.1 1.0 CB B:CYS261 3.1 99.8 1.0 CB C:CYS30 3.4 92.2 1.0 CB C:CYS25 3.7 86.1 1.0 CB C:CYS27 3.9 89.4 1.0 N C:CYS30 4.2 91.1 1.0 CA B:CYS261 4.3 99.5 1.0 C B:CYS261 4.4 99.6 1.0 CA C:CYS30 4.4 90.8 1.0 O B:CYS261 4.9 99.3 1.0 CA C:CYS25 4.9 87.0 1.0 C C:CYS25 4.9 87.0 1.0 O C:CYS25 5.0 87.2 1.0

T.H.Tahirov, N.D.Babayeva, K.Varzavand, J.J.Cooper, S.C.Sedore, D.H.Price. Crystal Structure of Hiv-1 Tat Complexed with Human P-Tefb. Nature V. 465 747 2010.
ISSN: ISSN 0028-0836
PubMed: 20535204
DOI: 10.1038/NATURE09131