Zinc in PDB 2ptw: Crystal Structure of the T. Brucei Enolase Complexed with Sulphate, Identification of A Metal Binding Site IV

All present enzymatic activity of Crystal Structure of the T. Brucei Enolase Complexed with Sulphate, Identification of A Metal Binding Site IV:
4.2.1.11;

The structure of Crystal Structure of the T. Brucei Enolase Complexed with Sulphate, Identification of A Metal Binding Site IV, PDB code: 2ptw was solved by M.V.A.S.Navarro, D.J.Rigden, R.C.Garratt, S.M.G.Dias, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.98 / 1.90
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	73.617, 111.259, 109.969, 90.00, 90.00, 90.00
R / Rfree (%)	21.4 / 25

The binding sites of Zinc atom in the Crystal Structure of the T. Brucei Enolase Complexed with Sulphate, Identification of A Metal Binding Site IV (pdb code 2ptw). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the T. Brucei Enolase Complexed with Sulphate, Identification of A Metal Binding Site IV, PDB code: 2ptw:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the T. Brucei Enolase Complexed with Sulphate, Identification of A Metal Binding Site IV


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the T. Brucei Enolase Complexed with Sulphate, Identification of A Metal Binding Site IV within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn500 b:36.7 occ:1.00 OD2 A:ASP318 2.1 27.1 1.0 OD2 A:ASP243 2.1 31.7 1.0 O A:HOH1000 2.1 25.8 1.0 OE2 A:GLU291 2.1 24.1 1.0 CG A:ASP243 3.0 35.8 1.0 CD A:GLU291 3.0 26.2 1.0 CG A:ASP318 3.1 27.2 1.0 OD1 A:ASP243 3.3 39.6 1.0 CB A:ASP318 3.5 29.6 1.0 CG A:GLU291 3.8 29.1 1.0 OD2 A:ASP292 3.8 38.6 1.0 OE1 A:GLU291 3.8 25.4 1.0 NZ A:LYS394 3.9 23.4 1.0 O A:HOH722 4.1 28.1 1.0 OD1 A:ASP318 4.2 27.8 1.0 CB A:ASP243 4.4 35.2 1.0 CD2 A:LEU341 4.5 21.8 1.0 NE2 A:GLN164 4.5 39.6 1.0 O A:HOH809 4.6 21.7 1.0 CG A:ASP292 4.8 38.0 1.0 NZ A:LYS343 4.8 22.9 1.0 CB A:GLU291 4.8 29.9 1.0 CA A:ASP318 5.0 30.9 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the T. Brucei Enolase Complexed with Sulphate, Identification of A Metal Binding Site IV


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the T. Brucei Enolase Complexed with Sulphate, Identification of A Metal Binding Site IV within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn501 b:41.3 occ:1.00 O A:HOH866 2.2 21.0 1.0 O A:HOH887 2.3 36.2 1.0 O A:HOH886 2.3 19.9 1.0 NE2 A:HIS156 2.3 39.2 1.0 O A:HOH888 2.3 33.5 1.0 O A:HOH881 2.4 26.5 1.0 CD2 A:HIS156 3.2 41.4 1.0 CE1 A:HIS156 3.3 35.9 1.0 OE1 A:GLN164 4.2 44.4 1.0 ND1 A:HIS156 4.4 38.1 1.0 CG A:HIS156 4.4 39.6 1.0 O2 A:SO4600 4.4 36.7 1.0 O4 A:SO4600 4.5 37.5 1.0 O A:HOH973 4.5 34.5 1.0 CD A:GLN164 4.6 40.2 1.0 O A:HOH779 4.6 31.9 1.0 NE2 A:GLN164 4.6 39.6 1.0 O A:HOH722 5.0 28.1 1.0 CB A:ALA39 5.0 40.1 1.0 S A:SO4600 5.0 40.9 1.0

M.V.Navarro, S.M.Gomes Dias, L.V.Mello, M.T.Da Silva Giotto, S.Gavalda, C.Blonski, R.C.Garratt, D.J.Rigden. Structural Flexibility in Trypanosoma Brucei Enolase Revealed By X-Ray Crystallography and Molecular Dynamics. Febs J. V. 274 5077 2007.
ISSN: ISSN 1742-464X
PubMed: 17822439
DOI: 10.1111/J.1742-4658.2007.06027.X