Atomistry » Zinc » PDB 2n9o-2nwo » 2nnx
Atomistry »
  Zinc »
    PDB 2n9o-2nwo »
      2nnx »

Zinc in PDB 2nnx: Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase

Enzymatic activity of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase

All present enzymatic activity of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase:
1.15.1.1;

Protein crystallography data

The structure of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase, PDB code: 2nnx was solved by J.P.Schuermann, P.J.Hart, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.00 / 2.30
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 76.471, 63.549, 85.105, 90.00, 116.05, 90.00
R / Rfree (%) 18.6 / 24.1

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase (pdb code 2nnx). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase, PDB code: 2nnx:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Zinc binding site 1 out of 8 in 2nnx

Go back to Zinc Binding Sites List in 2nnx
Zinc binding site 1 out of 8 in the Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn155

b:25.6
occ:1.00
 OD1 A:ASP83 1.9 25.4 1.0
ND1 A:HIS80 2.1 21.8 1.0
ND1 A:HIS71 2.1 21.6 1.0
ND1 A:HIS63 2.2 21.7 1.0
CG A:ASP83 2.7 25.6 1.0
OD2 A:ASP83 2.8 26.8 1.0
CE1 A:HIS71 2.9 24.4 1.0
CE1 A:HIS80 3.0 21.0 1.0
CG A:HIS80 3.1 22.6 1.0
CG A:HIS63 3.1 24.4 1.0
CE1 A:HIS63 3.2 23.9 1.0
CG A:HIS71 3.2 23.8 1.0
CB A:HIS63 3.4 24.5 1.0
CB A:HIS80 3.5 23.7 1.0
CB A:HIS71 3.7 24.7 1.0
CA A:HIS71 4.0 24.9 1.0
NE2 A:HIS80 4.1 22.1 1.0
NE2 A:HIS71 4.1 23.6 1.0
O A:LYS136 4.2 32.5 1.0
CB A:ASP83 4.2 25.4 1.0
CD2 A:HIS80 4.2 22.4 1.0
CD2 A:HIS71 4.3 22.9 1.0
CD2 A:HIS63 4.3 24.5 1.0
NE2 A:HIS63 4.3 25.2 1.0
N A:HIS80 4.6 24.9 1.0
CA A:HIS80 4.7 24.2 1.0
CA A:ASP83 4.7 25.4 1.0
O A:HOH170 4.8 21.6 1.0
N A:GLY72 4.8 25.2 1.0
CA A:HIS63 4.9 25.0 1.0
N A:HIS71 4.9 25.5 1.0
C A:HIS71 5.0 25.0 1.0
N A:ASP83 5.0 24.8 1.0

Zinc binding site 2 out of 8 in 2nnx

Go back to Zinc Binding Sites List in 2nnx
Zinc binding site 2 out of 8 in the Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn156

b:67.8
occ:1.00
 OE2 A:GLU77 1.9 34.9 1.0
CD A:GLU77 2.9 33.3 1.0
OE1 A:GLU77 3.4 34.3 1.0
CG A:GLU77 4.0 30.7 1.0

Zinc binding site 3 out of 8 in 2nnx

Go back to Zinc Binding Sites List in 2nnx
Zinc binding site 3 out of 8 in the Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn155

b:28.5
occ:1.00
 OD1 B:ASP83 1.8 26.1 1.0
ND1 B:HIS80 2.0 22.1 1.0
ND1 B:HIS71 2.2 22.5 1.0
ND1 B:HIS63 2.2 25.0 1.0
CG B:ASP83 2.7 25.5 1.0
CE1 B:HIS80 2.8 21.7 1.0
OD2 B:ASP83 3.0 25.1 1.0
CE1 B:HIS71 3.0 24.1 1.0
CG B:HIS63 3.1 25.8 1.0
CG B:HIS80 3.1 22.1 1.0
CE1 B:HIS63 3.1 23.9 1.0
CG B:HIS71 3.3 23.2 1.0
CB B:HIS63 3.4 25.4 1.0
CB B:HIS80 3.6 23.1 1.0
CB B:HIS71 3.6 24.1 1.0
CA B:HIS71 3.9 24.6 1.0
NE2 B:HIS80 4.0 20.8 1.0
CB B:ASP83 4.1 25.0 1.0
CD2 B:HIS80 4.1 21.1 1.0
O B:LYS136 4.2 33.6 1.0
NE2 B:HIS71 4.2 23.6 1.0
NE2 B:HIS63 4.2 25.5 1.0
CD2 B:HIS63 4.2 24.6 1.0
CD2 B:HIS71 4.3 21.1 1.0
CA B:ASP83 4.6 25.8 1.0
N B:HIS80 4.8 24.2 1.0
N B:GLY72 4.8 25.2 1.0
CA B:HIS80 4.8 23.7 1.0
N B:ASP83 4.8 25.5 1.0
N B:HIS71 4.9 24.8 1.0
C B:HIS71 4.9 24.9 1.0
CA B:HIS63 4.9 25.6 1.0

Zinc binding site 4 out of 8 in 2nnx

Go back to Zinc Binding Sites List in 2nnx
Zinc binding site 4 out of 8 in the Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn156

b:64.5
occ:1.00
 OE2 B:GLU77 2.5 33.4 1.0
OE1 B:GLU77 3.0 30.9 1.0
CD B:GLU77 3.1 31.6 1.0
CG B:GLU77 4.6 29.3 1.0
O B:HOH221 4.8 41.2 1.0

Zinc binding site 5 out of 8 in 2nnx

Go back to Zinc Binding Sites List in 2nnx
Zinc binding site 5 out of 8 in the Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn155

b:31.4
occ:1.00
 OD1 C:ASP83 1.9 22.9 1.0
ND1 C:HIS80 2.1 22.7 1.0
ND1 C:HIS63 2.2 24.2 1.0
ND1 C:HIS71 2.2 22.5 1.0
CG C:ASP83 2.7 24.7 1.0
OD2 C:ASP83 2.8 24.7 1.0
CE1 C:HIS80 3.0 22.2 1.0
CE1 C:HIS71 3.0 24.0 1.0
CG C:HIS80 3.1 22.6 1.0
CG C:HIS63 3.2 24.1 1.0
CE1 C:HIS63 3.2 24.4 1.0
CG C:HIS71 3.3 23.8 1.0
CB C:HIS63 3.4 24.5 1.0
CB C:HIS80 3.5 23.9 1.0
CB C:HIS71 3.8 24.7 1.0
CA C:HIS71 4.0 25.3 1.0
O C:LYS136 4.0 33.7 1.0
NE2 C:HIS80 4.1 22.9 1.0
CB C:ASP83 4.1 24.7 1.0
CD2 C:HIS80 4.2 21.7 1.0
NE2 C:HIS71 4.2 23.5 1.0
NE2 C:HIS63 4.3 26.0 1.0
CD2 C:HIS63 4.3 24.9 1.0
CD2 C:HIS71 4.4 22.8 1.0
CA C:ASP83 4.6 25.0 1.0
N C:HIS80 4.7 24.6 1.0
CA C:HIS80 4.7 24.3 1.0
N C:GLY72 4.8 25.6 1.0
N C:ASP83 4.9 24.9 1.0
CA C:HIS63 4.9 24.2 1.0
C C:HIS71 5.0 25.6 1.0

Zinc binding site 6 out of 8 in 2nnx

Go back to Zinc Binding Sites List in 2nnx
Zinc binding site 6 out of 8 in the Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn156

b:46.9
occ:1.00
 OE2 C:GLU77 2.0 32.5 1.0
CD C:GLU77 2.9 31.9 1.0
OE1 C:GLU77 3.1 31.6 1.0
CG C:GLU77 4.3 29.6 1.0

Zinc binding site 7 out of 8 in 2nnx

Go back to Zinc Binding Sites List in 2nnx
Zinc binding site 7 out of 8 in the Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn155

b:28.5
occ:1.00
 OD1 D:ASP83 2.0 25.5 1.0
ND1 D:HIS80 2.1 22.5 1.0
ND1 D:HIS71 2.1 21.8 1.0
ND1 D:HIS63 2.2 24.7 1.0
CG D:ASP83 2.8 24.9 1.0
CE1 D:HIS80 2.9 21.5 1.0
OD2 D:ASP83 2.9 25.1 1.0
CE1 D:HIS71 3.0 24.1 1.0
CG D:HIS63 3.2 25.6 1.0
CG D:HIS80 3.2 22.5 1.0
CE1 D:HIS63 3.2 24.5 1.0
CG D:HIS71 3.2 23.9 1.0
CB D:HIS63 3.4 24.9 1.0
CB D:HIS80 3.6 23.3 1.0
CB D:HIS71 3.7 24.4 1.0
CA D:HIS71 4.0 25.2 1.0
NE2 D:HIS80 4.0 22.2 1.0
O D:LYS136 4.0 32.9 1.0
NE2 D:HIS71 4.2 21.5 1.0
CD2 D:HIS80 4.2 22.3 1.0
CB D:ASP83 4.2 24.5 1.0
NE2 D:HIS63 4.3 26.2 1.0
CD2 D:HIS63 4.3 25.9 1.0
CD2 D:HIS71 4.3 22.2 1.0
N D:HIS80 4.7 24.6 1.0
CA D:ASP83 4.7 25.0 1.0
CA D:HIS80 4.8 23.6 1.0
N D:GLY72 4.9 25.8 1.0
N D:ASP83 4.9 25.1 1.0
CA D:HIS63 4.9 25.0 1.0
O D:HOH1042 4.9 37.1 1.0
N D:HIS71 4.9 25.6 1.0
C D:HIS71 5.0 25.6 1.0
C D:LYS136 5.0 33.5 1.0

Zinc binding site 8 out of 8 in 2nnx

Go back to Zinc Binding Sites List in 2nnx
Zinc binding site 8 out of 8 in the Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Crystal Structure of the H46R, H48Q Double Mutant of Human [Cu-Zn] Superoxide Dismutase within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Zn156

b:47.0
occ:1.00
 O D:HOH1054 2.3 36.3 1.0
OE1 D:GLU77 2.3 31.1 1.0
CD D:GLU77 3.2 32.0 1.0
OE2 D:GLU77 3.5 33.5 1.0
CG D:GLU77 4.5 29.9 1.0
CB D:GLU77 5.0 28.4 1.0

Reference:

J.Wang, A.Caruano-Yzermans, A.Rodriguez, J.P.Scheurmann, H.H.Slunt, X.Cao, J.Gitlin, P.J.Hart, D.R.Borchelt. Disease-Associated Mutations at Copper Ligand Histidine Residues of Superoxide Dismutase 1 Diminish the Binding of Copper and Compromise Dimer Stability J.Biol.Chem. V. 282 345 2007.
ISSN: ISSN 0021-9258
PubMed: 17092942
DOI: 10.1074/JBC.M604503200
Page generated: Thu Oct 17 02:15:28 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy