Zinc in PDB 2fps: Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model.

All present enzymatic activity of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model.:
3.1.3.15;

The structure of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model., PDB code: 2fps was solved by E.S.Rangarajan, M.Cygler, A.Matte, Montreal-Kingston Bacterialstructural Genomics Initiative (Bsgi), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.20
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	52.748, 131.325, 104.898, 90.00, 90.00, 90.00
R / Rfree (%)	17 / 24.4

The structure of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model. also contains other interesting chemical elements:

Chlorine	(Cl)	2 atoms
Calcium	(Ca)	2 atoms

The binding sites of Zinc atom in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model. (pdb code 2fps). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model., PDB code: 2fps:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model. within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn502 b:33.3 occ:1.00 ND1 A:HIS96 1.9 25.2 1.0 SG A:CYS104 2.3 31.1 1.0 SG A:CYS94 2.4 27.4 1.0 SG A:CYS102 2.4 31.9 1.0 CE1 A:HIS96 2.7 27.8 1.0 CG A:HIS96 3.0 25.9 1.0 CB A:CYS102 3.3 34.8 1.0 CB A:CYS94 3.5 26.7 1.0 CB A:CYS104 3.5 30.1 1.0 CB A:HIS96 3.5 25.6 1.0 N A:ARG105 3.6 28.6 1.0 CA A:CYS104 3.8 30.6 1.0 C A:CYS104 3.8 29.5 1.0 N A:CYS104 3.8 31.5 1.0 NE2 A:HIS96 3.9 28.0 1.0 CA A:CYS94 4.0 26.7 1.0 CD2 A:HIS96 4.1 26.1 1.0 CD A:PRO95 4.1 26.0 1.0 N A:HIS96 4.1 25.4 1.0 CA A:ARG105 4.3 27.0 1.0 CB A:ARG105 4.4 27.8 1.0 N A:PRO95 4.4 26.1 1.0 C A:CYS94 4.4 26.6 1.0 CA A:HIS96 4.5 25.9 1.0 O A:HOH571 4.5 27.7 1.0 O A:CYS104 4.5 29.1 1.0 CA A:CYS102 4.7 34.8 1.0 C A:CYS102 4.9 34.2 1.0 O A:HOH546 5.0 19.7 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of the N-Terminal Domain of E.Coli Hisb- Apo Ca Model. within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn501 b:23.6 occ:1.00 ND1 B:HIS96 2.1 16.1 1.0 SG B:CYS94 2.3 17.6 1.0 SG B:CYS104 2.3 22.6 1.0 SG B:CYS102 2.3 23.5 1.0 CE1 B:HIS96 3.1 15.0 1.0 CG B:HIS96 3.1 17.1 1.0 CB B:CYS102 3.1 24.6 1.0 CB B:CYS94 3.4 16.3 1.0 CB B:HIS96 3.4 18.6 1.0 CB B:CYS104 3.5 22.0 1.0 N B:ARG105 3.6 22.2 1.0 C B:CYS104 3.8 22.0 1.0 N B:CYS104 3.8 22.4 1.0 CA B:CYS104 3.8 22.3 1.0 CA B:CYS94 4.1 16.5 1.0 CA B:ARG105 4.2 21.9 1.0 NE2 B:HIS96 4.2 14.3 1.0 CD2 B:HIS96 4.2 15.4 1.0 CB B:ARG105 4.2 21.6 1.0 N B:HIS96 4.3 17.9 1.0 CA B:HIS96 4.5 19.3 1.0 O B:HOH540 4.5 20.1 1.0 CA B:CYS102 4.5 24.7 1.0 CD B:PRO95 4.5 15.5 1.0 O B:CYS104 4.5 22.1 1.0 C B:CYS94 4.5 16.6 1.0 N B:PRO95 4.6 16.3 1.0 C B:CYS102 4.7 24.2 1.0 N B:ASP103 4.9 24.1 1.0 O B:HOH630 5.0 24.7 1.0 O B:HOH518 5.0 14.3 1.0

E.S.Rangarajan, A.Proteau, J.Wagner, M.N.Hung, A.Matte, M.Cygler. Structural Snapshots of Escherichia Coli Histidinol Phosphate Phosphatase Along the Reaction Pathway. J.Biol.Chem. V. 281 37930 2006.
ISSN: ISSN 0021-9258
PubMed: 16966333
DOI: 10.1074/JBC.M604916200