Zinc in PDB 1zni: Insulin

The structure of Insulin, PDB code: 1zni was solved by M.G.W.Turkenburg, J.L.Whittingham, G.G.Dodson, E.J.Dodson, B.Xiao, G.A.Bentley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 1.50
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	80.700, 80.700, 37.600, 90.00, 90.00, 120.00
R / Rfree (%)	17.8 / n/a

The structure of Insulin also contains other interesting chemical elements:

Chlorine

(Cl)

3 atoms

The binding sites of Zinc atom in the Insulin (pdb code 1zni). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Insulin, PDB code: 1zni:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Insulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn31 b:16.3 occ:0.17 NE2 B:HIS10 2.0 14.9 0.5 CL B:CL33 2.3 21.3 0.2 CE1 B:HIS10 2.9 17.6 0.5 CD2 B:HIS10 3.0 13.9 0.5 ND1 B:HIS10 4.0 24.7 0.5 CG B:HIS10 4.1 14.7 0.5 O B:LEU6 4.7 19.4 1.0 CB B:LEU6 4.9 17.8 1.0

Zinc binding site 2 out of 3 in the Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Insulin within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn32 b:21.2 occ:0.50 NE2 B:HIS10 1.9 25.4 0.5 CL B:CL34 2.3 21.1 0.5 CL B:CL35 2.3 23.6 0.5 CE1 B:HIS10 2.8 23.7 0.5 CD2 B:HIS10 2.9 22.7 0.5 O A:HOH31 3.7 42.6 1.0 ND1 B:HIS10 3.9 17.6 0.5 CG B:HIS10 4.0 14.2 0.5 O A:HOH24 4.4 31.1 1.0 CB B:ALA14 4.7 18.2 1.0

Zinc binding site 3 out of 3 in the Insulin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Insulin within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn31 b:18.6 occ:0.33 NE2 D:HIS10 2.1 15.1 1.0 O D:HOH32 2.3 30.0 1.0 CE1 D:HIS10 3.0 16.8 1.0 CD2 D:HIS10 3.1 18.4 1.0 O D:HOH58 4.1 82.9 0.3 CG D:HIS10 4.2 15.9 1.0 ND1 D:HIS10 4.2 17.3 1.0

G.Bentley, E.Dodson, G.Dodson, D.Hodgkin, D.Mercola. Structure of Insulin in 4-Zinc Insulin. Nature V. 261 166 1976.
ISSN: ISSN 0028-0836
PubMed: 1272390
DOI: 10.1038/261166A0