Zinc in PDB 1kys: Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor

The structure of Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor, PDB code: 1kys was solved by D.P.Barondeau, C.J.Kassmann, J.A.Tainer, E.D.Getzoff, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.44
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.147, 62.226, 68.817, 90.00, 90.00, 90.00
R / Rfree (%)	15.7 / 22.9

The binding sites of Zinc atom in the Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor (pdb code 1kys). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor, PDB code: 1kys:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn304 b:36.5 occ:1.00 O A:HOH1210 2.0 44.6 1.0 ND1 A:HIS25 2.1 27.0 1.0 CE1 A:HIS25 2.9 27.9 1.0 CG A:HIS25 3.2 19.7 1.0 CB A:HIS25 3.6 21.4 1.0 CA A:HIS25 3.9 22.0 1.0 NE2 A:HIS25 4.1 26.3 1.0 O A:HOH1112 4.1 34.1 1.0 CD2 A:HIS25 4.2 23.2 1.0 O A:HOH1057 4.3 30.8 1.0 N A:HIS25 4.7 20.1 1.0 O A:GLY24 5.0 25.6 1.0

Zinc binding site 2 out of 4 in the Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn306 b:27.5 occ:1.00 OE2 A:GLU142 2.0 18.4 1.0 O A:HOH1042 2.0 23.7 1.0 O A:HOH1051 2.1 22.6 1.0 O A:HOH1075 2.2 28.9 1.0 CD A:GLU142 2.9 14.8 1.0 OE1 A:GLU142 3.2 22.7 1.0 O A:ILE171 4.0 17.1 1.0 CG A:GLU142 4.3 15.7 1.0 CA A:GLY174 4.3 20.1 1.0 O A:HOH1036 4.3 24.1 1.0 O A:HOH1148 4.4 43.1 1.0 N A:GLY174 4.6 20.2 1.0 O A:GLU172 4.8 35.4 1.0 CB A:ASN170 4.8 16.3 1.0

Zinc binding site 3 out of 4 in the Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn308 b:45.3 occ:1.00 OE2 A:GLU172 2.1 37.0 1.0 O A:HOH1101 2.2 30.3 1.0 OE1 A:GLU172 2.3 45.5 1.0 CD A:GLU172 2.5 40.1 1.0 O A:HIS139 3.4 19.7 1.0 O A:HOH1234 3.5 57.5 1.0 CG A:GLU172 4.0 36.3 1.0 C A:HIS139 4.6 18.5 1.0 CA A:LYS140 4.6 17.6 1.0 CB A:GLU172 4.8 30.5 1.0

Zinc binding site 4 out of 4 in the Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of A Zn-Bound Green Fluorescent Protein Biosensor within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn301 b:42.5 occ:1.00 ND1 A:CRG66 2.0 19.5 1.0 N2 A:CRG66 2.1 13.1 1.0 OE2 A:GLU222 2.1 42.9 1.0 O A:HOH1246 2.1 57.0 1.0 OG1 A:CRG66 2.3 17.4 1.0 CB1 A:CRG66 2.9 17.0 1.0 CG2 A:CRG66 3.0 18.5 1.0 CA2 A:CRG66 3.0 15.4 1.0 CD A:GLU222 3.0 33.3 1.0 C1 A:CRG66 3.0 13.1 1.0 CE1 A:CRG66 3.1 20.0 1.0 OE1 A:GLU222 3.2 40.4 1.0 CB2 A:CRG66 3.3 19.8 1.0 CA1 A:CRG66 3.6 14.4 1.0 O A:HOH1028 3.7 20.3 1.0 O A:VAL61 4.0 13.7 1.0 CD2 A:CRG66 4.1 18.0 1.0 N3 A:CRG66 4.2 13.1 1.0 C2 A:CRG66 4.2 13.0 1.0 NE2 A:CRG66 4.3 20.4 1.0 CG1 A:CRG66 4.3 20.9 1.0 CG1 A:VAL61 4.3 13.5 1.0 CG A:GLU222 4.4 27.1 1.0 N1 A:CRG66 4.4 13.7 1.0 CA A:THR62 4.8 11.2 1.0 C A:VAL61 4.8 11.2 1.0 CD1 A:LEU42 4.8 33.2 1.0 O A:HOH1149 4.8 37.5 1.0 CB A:GLU222 4.9 17.7 1.0

D.P.Barondeau, C.J.Kassmann, J.A.Tainer, E.D.Getzoff. Structural Chemistry of A Green Fluorescent Protein Zn Biosensor. J.Am.Chem.Soc. V. 124 3522 2002.
ISSN: ISSN 0002-7863
PubMed: 11929238
DOI: 10.1021/JA0176954