Zinc in PDB 8wml: Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat

The binding sites of Zinc atom in the Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat (pdb code 8wml). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat, PDB code: 8wml:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1701 b:161.8 occ:1.00 SG A:CYS126 2.3 112.5 1.0 SG A:CYS115 3.0 131.5 1.0 CA A:GLY88 3.6 116.8 1.0 NH1 A:ARG131 3.7 119.1 1.0 SG A:CYS86 3.8 116.6 1.0 CB A:CYS126 3.8 109.6 1.0 N A:LYS89 3.9 112.9 1.0 C A:GLY88 4.1 116.6 1.0 CZ A:ARG131 4.2 119.2 1.0 NH2 A:ARG131 4.4 118.2 1.0 N A:GLY88 4.5 118.1 1.0 CB A:LYS89 4.5 114.4 1.0 N A:CYS115 4.6 128.7 1.0 CB A:CYS115 4.6 128.1 1.0 CA A:CYS126 4.8 109.0 1.0 O A:CYS115 4.8 127.9 1.0 CA A:LYS89 4.8 113.8 1.0 NE A:ARG131 5.0 120.6 1.0

Zinc binding site 2 out of 4 in the Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1702 b:120.1 occ:1.00 SG A:CYS472 2.3 74.6 1.0 SG A:CYS463 2.3 81.1 1.0 CB A:CYS472 2.9 67.5 1.0 SG A:CYS477 3.0 60.5 1.0 CB A:ALA465 3.3 73.2 1.0 SG A:CYS474 3.6 55.8 1.0 CB A:CYS463 4.0 68.9 1.0 CB A:CYS477 4.2 50.7 1.0 CA A:ALA465 4.4 70.1 1.0 CA A:CYS472 4.4 64.0 1.0 N A:ALA465 4.5 69.3 1.0 CB A:CYS474 4.7 51.8 1.0 CA A:CYS463 4.8 68.6 1.0

Zinc binding site 3 out of 4 in the Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1703 b:94.0 occ:1.00 SG A:CYS708 2.3 45.9 1.0 SG A:CYS706 2.3 58.8 1.0 SG A:CYS711 2.3 63.6 1.0 CB A:CYS706 3.5 57.2 1.0 CB A:CYS711 3.5 40.7 1.0 CB A:CYS708 3.8 42.0 1.0 N A:CYS711 4.3 39.6 1.0 N A:CYS708 4.4 49.1 1.0 CA A:CYS711 4.5 34.5 1.0 CA A:CYS708 4.7 45.8 1.0 CA A:CYS706 4.8 55.3 1.0 C A:CYS706 5.0 55.2 1.0

Zinc binding site 4 out of 4 in the Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Cryo-Em Structure of CAS7-11-Crrna Bound to N-Terminal of Tpr-Chat within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1704 b:76.7 occ:1.00 SG A:CYS1342 2.3 35.8 1.0 SG A:CYS1312 2.3 34.0 1.0 SG A:CYS965 2.3 39.8 1.0 SG A:CYS1345 2.3 28.5 1.0 CB A:CYS1345 3.5 21.6 1.0 CB A:CYS965 3.6 25.4 1.0 CB A:CYS1342 3.6 23.9 1.0 CB A:CYS1312 3.7 27.4 1.0 N A:CYS1312 4.1 32.1 1.0 N A:CYS1342 4.1 19.1 1.0 N A:CYS1345 4.3 23.6 1.0 CA A:CYS1342 4.4 16.5 1.0 N A:CYS965 4.4 35.9 1.0 CA A:CYS1345 4.5 15.1 1.0 CA A:CYS1312 4.5 26.8 1.0 CA A:CYS965 4.6 28.2 1.0 C A:CYS1342 4.9 22.7 1.0 O A:CYS1342 4.9 27.1 1.0 NH2 A:ARG876 5.0 33.3 1.0

T.Hong, Q.Luo, H.Ma, X.Wang, X.Li, C.Shen, J.Pang, Y.Wang, Y.Chen, C.Zhang, Z.Su, H.Dong, X.Tang. Structural Basis of Negative Regulation of Crispr-CAS7-11 By Tpr-Chat. Signal Transduct Target Ther V. 9 111 2024.
ISSN: ESSN 2059-3635
PubMed: 38735995
DOI: 10.1038/S41392-024-01821-4