Zinc in PDB 8wmi: Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna

The binding sites of Zinc atom in the Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna (pdb code 8wmi). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna, PDB code: 8wmi:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1701 b:210.6 occ:1.00 SG A:CYS123 2.3 164.1 1.0 SG A:CYS126 2.3 164.1 1.0 SG A:CYS86 2.3 167.5 1.0 SG A:CYS115 2.3 183.2 1.0 CB A:CYS86 3.4 167.5 1.0 CB A:CYS126 3.5 164.1 1.0 CB A:CYS123 3.6 164.1 1.0 CB A:CYS115 3.8 183.2 1.0 N A:CYS126 4.2 164.1 1.0 CE2 A:TYR122 4.2 173.0 1.0 CD2 A:TYR122 4.3 173.0 1.0 N A:CYS86 4.4 167.5 1.0 CA A:CYS126 4.4 164.1 1.0 CA A:CYS86 4.5 167.5 1.0 N A:CYS115 4.8 183.2 1.0 CA A:CYS123 4.8 164.1 1.0 CA A:CYS115 4.9 183.2 1.0

Zinc binding site 2 out of 4 in the Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1702 b:134.8 occ:1.00 SG A:CYS472 2.3 117.2 1.0 SG A:CYS463 2.3 97.8 1.0 SG A:CYS474 2.3 106.0 1.0 SG A:CYS477 2.3 100.4 1.0 CB A:CYS477 3.4 100.4 1.0 CB A:CYS463 3.8 97.8 1.0 CB A:CYS472 3.9 117.2 1.0 CB A:CYS474 4.0 106.0 1.0 O A:CYS472 4.2 117.2 1.0 N A:CYS477 4.3 100.4 1.0 CA A:CYS477 4.4 100.4 1.0 CB A:ALA465 4.7 105.9 1.0 C A:CYS472 4.7 117.2 1.0 N A:CYS474 4.7 106.0 1.0 CA A:CYS472 4.8 117.2 1.0 CA A:CYS463 4.8 97.8 1.0 CA A:CYS474 4.9 106.0 1.0

Zinc binding site 3 out of 4 in the Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1703 b:148.1 occ:1.00 ND1 A:HIS703 2.1 107.8 1.0 SG A:CYS706 2.3 112.7 1.0 SG A:CYS708 2.3 99.2 1.0 SG A:CYS711 2.3 90.8 1.0 CE1 A:HIS703 2.8 107.8 1.0 CG A:HIS703 3.2 107.8 1.0 CB A:CYS708 3.2 99.2 1.0 CB A:HIS703 3.7 107.8 1.0 CB A:CYS706 3.7 112.7 1.0 CB A:CYS711 3.8 90.8 1.0 CA A:HIS703 3.8 107.8 1.0 N A:CYS708 4.0 99.2 1.0 NE2 A:HIS703 4.0 107.8 1.0 CD2 A:HIS703 4.2 107.8 1.0 CA A:CYS708 4.2 99.2 1.0 C A:HIS703 4.7 107.8 1.0 CA A:CYS706 4.8 112.7 1.0 C A:CYS706 4.8 112.7 1.0 N A:GLU707 4.8 105.4 1.0 N A:HIS703 4.9 107.8 1.0 N A:CYS711 5.0 90.8 1.0

Zinc binding site 4 out of 4 in the Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Cryo-Em Structure of DICAS7-11 Mutant in Complex with Crrna within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1704 b:114.1 occ:1.00 SG A:CYS965 2.3 79.0 1.0 SG A:CYS1312 2.3 87.6 1.0 SG A:CYS1342 2.3 78.2 1.0 CB A:CYS1312 2.7 87.6 1.0 CB A:CYS1342 2.9 78.2 1.0 CB A:CYS965 3.2 79.0 1.0 N A:CYS1342 3.4 78.2 1.0 CA A:CYS1342 3.7 78.2 1.0 NE A:ARG876 3.8 84.6 1.0 SG A:CYS1345 3.9 77.0 1.0 CA A:CYS1312 3.9 87.6 1.0 N A:CYS1312 4.0 87.6 1.0 N A:CYS965 4.4 79.0 1.0 NH2 A:ARG876 4.4 84.6 1.0 CA A:CYS965 4.4 79.0 1.0 CB A:CYS1345 4.4 77.0 1.0 O A:CYS1312 4.5 87.6 1.0 C A:LEU1341 4.6 79.1 1.0 CZ A:ARG876 4.6 84.6 1.0 CD A:ARG876 4.6 84.6 1.0 C A:CYS1312 4.8 87.6 1.0 C A:CYS1342 4.8 78.2 1.0 CA A:LEU1341 4.9 79.1 1.0

T.Hong, Q.Luo, H.Ma, X.Wang, X.Li, C.Shen, J.Pang, Y.Wang, Y.Chen, C.Zhang, Z.Su, H.Dong, X.Tang. Structural Basis of Negative Regulation of Crispr-CAS7-11 By Tpr-Chat. Signal Transduct Target Ther V. 9 111 2024.
ISSN: ESSN 2059-3635
PubMed: 38735995
DOI: 10.1038/S41392-024-01821-4