Zinc in PDB 8sf0: Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd)

The binding sites of Zinc atom in the Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd) (pdb code 8sf0). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd), PDB code: 8sf0:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd) within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn5102 b:58.1 occ:1.00 ND1 A:HIS4983 2.0 48.1 1.0 NE2 A:HIS4978 2.0 49.8 1.0 SG A:CYS4961 2.3 45.9 1.0 SG A:CYS4958 2.3 47.3 1.0 CG A:HIS4983 2.9 48.1 1.0 CE1 A:HIS4978 2.9 49.8 1.0 CB A:HIS4983 3.1 48.1 1.0 CE1 A:HIS4983 3.1 48.1 1.0 CD2 A:HIS4978 3.1 49.8 1.0 CB A:CYS4958 3.2 47.3 1.0 CB A:CYS4961 3.5 45.9 1.0 N A:CYS4961 3.7 45.9 1.0 ND1 A:HIS4978 4.0 49.8 1.0 CD2 A:HIS4983 4.0 48.1 1.0 NE2 A:HIS4983 4.1 48.1 1.0 CG A:HIS4978 4.2 49.8 1.0 CA A:CYS4961 4.2 45.9 1.0 CB A:ILE4960 4.5 44.6 1.0 CA A:HIS4983 4.6 48.1 1.0 CA A:CYS4958 4.6 47.3 1.0 CZ A:PHE4968 4.6 53.0 1.0 N A:ILE4960 4.7 44.6 1.0 C A:ILE4960 4.7 44.6 1.0 CA A:ILE4960 4.9 44.6 1.0 CE2 A:PHE4968 4.9 53.0 1.0 C A:CYS4958 4.9 47.3 1.0 C A:CYS4961 5.0 45.9 1.0 N A:GLY4962 5.0 48.9 1.0

Zinc binding site 2 out of 4 in the Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd) within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn5102 b:57.7 occ:1.00 ND1 B:HIS4983 2.0 48.2 1.0 NE2 B:HIS4978 2.0 50.3 1.0 SG B:CYS4961 2.3 45.7 1.0 SG B:CYS4958 2.3 47.4 1.0 CG B:HIS4983 2.9 48.2 1.0 CE1 B:HIS4978 2.9 50.3 1.0 CB B:HIS4983 3.1 48.2 1.0 CE1 B:HIS4983 3.1 48.2 1.0 CD2 B:HIS4978 3.1 50.3 1.0 CB B:CYS4958 3.2 47.4 1.0 CB B:CYS4961 3.5 45.7 1.0 N B:CYS4961 3.7 45.7 1.0 ND1 B:HIS4978 4.0 50.3 1.0 CD2 B:HIS4983 4.0 48.2 1.0 NE2 B:HIS4983 4.1 48.2 1.0 CG B:HIS4978 4.2 50.3 1.0 CA B:CYS4961 4.2 45.7 1.0 CB B:ILE4960 4.5 44.8 1.0 CA B:HIS4983 4.6 48.2 1.0 CA B:CYS4958 4.6 47.4 1.0 CZ B:PHE4968 4.7 53.5 1.0 N B:ILE4960 4.7 44.8 1.0 C B:ILE4960 4.7 44.8 1.0 CA B:ILE4960 4.9 44.8 1.0 CE2 B:PHE4968 4.9 53.5 1.0 C B:CYS4958 4.9 47.4 1.0 C B:CYS4961 5.0 45.7 1.0 N B:GLY4962 5.0 48.7 1.0

Zinc binding site 3 out of 4 in the Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd) within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn5102 b:56.5 occ:1.00 ND1 C:HIS4983 2.0 47.6 1.0 NE2 C:HIS4978 2.0 49.5 1.0 SG C:CYS4961 2.3 45.5 1.0 SG C:CYS4958 2.3 47.4 1.0 CG C:HIS4983 2.9 47.6 1.0 CE1 C:HIS4978 2.9 49.5 1.0 CB C:HIS4983 3.1 47.6 1.0 CE1 C:HIS4983 3.1 47.6 1.0 CD2 C:HIS4978 3.1 49.5 1.0 CB C:CYS4958 3.2 47.4 1.0 CB C:CYS4961 3.5 45.5 1.0 N C:CYS4961 3.7 45.5 1.0 ND1 C:HIS4978 4.0 49.5 1.0 CD2 C:HIS4983 4.0 47.6 1.0 NE2 C:HIS4983 4.1 47.6 1.0 CG C:HIS4978 4.2 49.5 1.0 CA C:CYS4961 4.2 45.5 1.0 CB C:ILE4960 4.5 44.6 1.0 CA C:HIS4983 4.6 47.6 1.0 CA C:CYS4958 4.6 47.4 1.0 CZ C:PHE4968 4.7 53.1 1.0 N C:ILE4960 4.7 44.6 1.0 C C:ILE4960 4.7 44.6 1.0 CA C:ILE4960 4.9 44.6 1.0 CE2 C:PHE4968 4.9 53.1 1.0 C C:CYS4958 4.9 47.4 1.0 C C:CYS4961 5.0 45.5 1.0 N C:GLY4962 5.0 48.9 1.0

Zinc binding site 4 out of 4 in the Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Cryo-Em Structure of RYR1 + Camp (Local Refinement of Tmd) within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn5102 b:57.9 occ:1.00 ND1 D:HIS4983 2.0 47.9 1.0 NE2 D:HIS4978 2.0 49.9 1.0 SG D:CYS4961 2.3 46.0 1.0 SG D:CYS4958 2.3 47.5 1.0 CG D:HIS4983 2.9 47.9 1.0 CE1 D:HIS4978 2.9 49.9 1.0 CB D:HIS4983 3.1 47.9 1.0 CE1 D:HIS4983 3.1 47.9 1.0 CD2 D:HIS4978 3.1 49.9 1.0 CB D:CYS4958 3.2 47.5 1.0 CB D:CYS4961 3.5 46.0 1.0 N D:CYS4961 3.7 46.0 1.0 ND1 D:HIS4978 4.0 49.9 1.0 CD2 D:HIS4983 4.0 47.9 1.0 NE2 D:HIS4983 4.1 47.9 1.0 CG D:HIS4978 4.2 49.9 1.0 CA D:CYS4961 4.2 46.0 1.0 CB D:ILE4960 4.5 44.9 1.0 CA D:HIS4983 4.6 47.9 1.0 CA D:CYS4958 4.6 47.5 1.0 CZ D:PHE4968 4.7 53.0 1.0 N D:ILE4960 4.7 44.9 1.0 C D:ILE4960 4.7 44.9 1.0 CA D:ILE4960 4.9 44.9 1.0 CE2 D:PHE4968 4.9 53.0 1.0 C D:CYS4958 4.9 47.5 1.0 C D:CYS4961 5.0 46.0 1.0 N D:GLY4962 5.0 49.1 1.0

S.Cholak, J.W.Saville, X.Zhu, A.M.Berezuk, K.S.Tuttle, O.Haji-Ghassemi, F.J.Alvarado, F.Van Petegem, S.Subramaniam. Allosteric Modulation of Ryanodine Receptor RYR1 By Nucleotide Derivatives Structure 2023.
ISSN: ISSN 0969-2126
DOI: 10.1016/J.STR.2023.04.009