Zinc in PDB 9guw: 30S-Tec (Tec in Expressome Position) Inactive State 2

All present enzymatic activity of 30S-Tec (Tec in Expressome Position) Inactive State 2:
2.7.7.6;

The structure of 30S-Tec (Tec in Expressome Position) Inactive State 2 also contains other interesting chemical elements:

Magnesium

(Mg)

118 atoms

The binding sites of Zinc atom in the 30S-Tec (Tec in Expressome Position) Inactive State 2 (pdb code 9guw). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the 30S-Tec (Tec in Expressome Position) Inactive State 2, PDB code: 9guw:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the 30S-Tec (Tec in Expressome Position) Inactive State 2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of 30S-Tec (Tec in Expressome Position) Inactive State 2 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn301 b:95.8 occ:1.00 NE2 C:HIS18 2.3 92.0 1.0 CE1 C:HIS18 2.5 92.0 1.0 OD1 C:ASP204 3.4 96.0 1.0 OD2 C:ASP204 3.4 96.0 1.0 OD1 C:ASP205 3.5 108.8 1.0 CD2 C:HIS18 3.6 92.0 1.0 ND2 C:ASN190 3.8 76.5 1.0 CG C:ASP204 3.8 96.0 1.0 ND1 C:HIS18 3.8 92.0 1.0 OD2 C:ASP205 3.9 108.8 1.0 OD2 C:ASP165 3.9 78.2 1.0 OD1 C:ASN190 4.0 76.5 1.0 OD1 C:ASP165 4.1 78.2 1.0 CG C:ASP205 4.1 108.8 1.0 CG C:ASP165 4.2 78.2 1.0 OD2 C:ASP188 4.2 83.5 1.0 CG C:ASN190 4.3 76.5 1.0 CG C:HIS18 4.4 92.0 1.0 CB C:ASP188 4.6 83.5 1.0 NZ C:LYS73 4.6 78.7 1.0 CA B:GLY80 4.7 134.1 1.0 O B:PHE79 4.8 137.4 1.0 CE C:LYS73 4.8 78.7 1.0 CG C:ASP188 4.8 83.5 1.0 C B:PHE79 4.8 137.4 1.0 N B:GLY80 4.8 134.1 1.0

Zinc binding site 2 out of 3 in the 30S-Tec (Tec in Expressome Position) Inactive State 2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of 30S-Tec (Tec in Expressome Position) Inactive State 2 within 5.0Å range: probe atom residue distance (Å) B Occ 4:Zn1501 b:188.1 occ:1.00 CB 4:CYS70 2.1 191.9 1.0 SG 4:CYS70 2.3 191.9 1.0 SG 4:CYS72 2.3 187.7 1.0 SG 4:CYS85 3.0 179.3 1.0 CG1 4:VAL90 3.1 183.2 1.0 N 4:CYS72 3.1 187.7 1.0 CB 4:CYS72 3.3 187.7 1.0 CA 4:CYS70 3.3 191.9 1.0 CB 4:CYS85 3.4 179.3 1.0 N 4:LEU71 3.5 188.9 1.0 C 4:CYS70 3.5 191.9 1.0 CA 4:CYS72 3.7 187.7 1.0 N 4:GLY73 4.0 190.7 1.0 C 4:LEU71 4.1 188.9 1.0 C 4:CYS72 4.2 187.7 1.0 O 4:VAL90 4.3 183.2 1.0 O 4:CYS70 4.3 191.9 1.0 CA 4:LEU71 4.4 188.9 1.0 N 4:CYS70 4.5 191.9 1.0 CB 4:VAL90 4.6 183.2 1.0 N 4:LYS74 4.6 186.2 1.0 CB 4:CYS88 4.7 183.5 1.0 CA 4:CYS85 4.9 179.3 1.0 N 4:VAL90 5.0 183.2 1.0 C 4:VAL90 5.0 183.2 1.0 CB 4:LEU71 5.0 188.9 1.0

Zinc binding site 3 out of 3 in the 30S-Tec (Tec in Expressome Position) Inactive State 2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of 30S-Tec (Tec in Expressome Position) Inactive State 2 within 5.0Å range: probe atom residue distance (Å) B Occ 4:Zn1502 b:138.3 occ:1.00 SG 4:CYS888 2.3 130.0 1.0 SG 4:CYS898 2.3 128.6 1.0 SG 4:CYS814 2.3 132.8 1.0 SG 4:CYS895 2.3 125.5 1.0 NH2 4:ARG883 2.6 135.7 1.0 CB 4:CYS895 2.7 125.5 1.0 CB 4:CYS888 2.9 130.0 1.0 CB 4:CYS814 3.2 132.8 1.0 CB 4:CYS898 3.6 128.6 1.0 CA 4:CYS888 3.7 130.0 1.0 CZ 4:ARG883 3.8 135.7 1.0 N 4:CYS814 3.9 132.8 1.0 CA 4:CYS895 3.9 125.5 1.0 N 4:CYS898 4.0 128.6 1.0 CA 4:CYS814 4.1 132.8 1.0 N 4:CYS895 4.2 125.5 1.0 NE 4:ARG883 4.2 135.7 1.0 C 4:CYS895 4.3 125.5 1.0 CA 4:CYS898 4.4 128.6 1.0 O 4:CYS895 4.4 125.5 1.0 C 4:CYS888 4.6 130.0 1.0 N 4:ASP889 4.6 130.5 1.0 OG1 4:THR890 4.6 124.9 1.0 NH1 4:ARG883 4.8 135.7 1.0 N 4:CYS888 4.9 130.0 1.0 CG2 4:THR816 4.9 139.7 1.0 OD1 4:ASP889 5.0 130.5 1.0

M.W.Webster, A.Chauvier, H.Rahil, A.Graziadei, N.Miropolskaya, K.Charles, M.Takacs, C.Saintandre, J.Rappsilber, N.G.Walter, A.Weixlbaumer. Molecular Basis of Mrna Delivery to the Bacterial Ribosome Science 2024.
ISSN: ESSN 1095-9203
DOI: 10.1126/SCIENCE.ADO8476