Zinc in PDB 8ph9: E. Coli Rna Polymerase Paused at Ops Site (Non-Complementary Scaffold)

All present enzymatic activity of E. Coli Rna Polymerase Paused at Ops Site (Non-Complementary Scaffold):
2.7.7.6;

The structure of E. Coli Rna Polymerase Paused at Ops Site (Non-Complementary Scaffold) also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Zinc atom in the E. Coli Rna Polymerase Paused at Ops Site (Non-Complementary Scaffold) (pdb code 8ph9). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the E. Coli Rna Polymerase Paused at Ops Site (Non-Complementary Scaffold), PDB code: 8ph9:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the E. Coli Rna Polymerase Paused at Ops Site (Non-Complementary Scaffold)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of E. Coli Rna Polymerase Paused at Ops Site (Non-Complementary Scaffold) within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1501 b:185.3 occ:1.00 SG J:CYS88 2.3 133.1 1.0 SG J:CYS70 2.3 140.1 1.0 SG J:CYS85 2.3 132.5 1.0 SG J:CYS72 2.3 130.5 1.0 CB J:CYS72 3.0 125.2 1.0 CB J:CYS85 3.0 124.6 1.0 N J:CYS72 3.6 127.0 1.0 CB J:CYS70 3.7 129.7 1.0 CA J:CYS72 3.9 125.9 1.0 CB J:CYS88 4.0 123.6 1.0 N J:CYS88 4.0 127.4 1.0 N J:GLY73 4.4 128.0 1.0 CA J:CYS88 4.5 125.6 1.0 CA J:CYS85 4.5 123.1 1.0 CB J:LYS87 4.5 130.7 1.0 N J:LEU71 4.6 119.8 1.0 C J:CYS72 4.6 125.7 1.0 N J:LYS74 4.7 126.6 1.0 N J:LYS87 4.8 128.8 1.0 CB J:LYS74 4.8 123.3 1.0 CA J:CYS70 4.8 127.1 1.0 C J:LEU71 4.8 121.7 1.0 C J:CYS70 4.9 128.8 1.0 C J:LYS87 4.9 131.8 1.0 N J:GLY89 4.9 117.9 1.0 CA J:LYS87 5.0 130.2 1.0 C J:CYS85 5.0 128.3 1.0 C J:CYS88 5.0 124.8 1.0 CB J:VAL90 5.0 101.7 1.0

Zinc binding site 2 out of 2 in the E. Coli Rna Polymerase Paused at Ops Site (Non-Complementary Scaffold)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of E. Coli Rna Polymerase Paused at Ops Site (Non-Complementary Scaffold) within 5.0Å range: probe atom residue distance (Å) B Occ J:Zn1502 b:150.6 occ:1.00 SG J:CYS814 2.3 109.0 1.0 SG J:CYS888 2.3 113.4 1.0 SG J:CYS898 2.3 103.3 1.0 SG J:CYS895 2.3 99.5 1.0 CB J:CYS898 2.5 84.6 1.0 CB J:CYS895 3.0 76.7 1.0 CB J:CYS814 3.7 89.3 1.0 CB J:CYS888 3.7 97.7 1.0 CA J:CYS898 3.7 76.2 1.0 N J:CYS898 3.8 82.9 1.0 N J:CYS895 3.8 77.8 1.0 CA J:CYS888 3.9 94.7 1.0 CA J:CYS895 3.9 74.7 1.0 N J:CYS814 4.2 99.5 1.0 NH2 J:ARG883 4.3 81.2 1.0 N J:ASP889 4.3 90.6 1.0 OG1 J:THR890 4.5 97.7 1.0 C J:CYS895 4.5 80.5 1.0 O J:CYS895 4.5 90.3 1.0 CA J:CYS814 4.6 93.3 1.0 C J:CYS888 4.6 97.6 1.0 C J:CYS898 4.8 79.2 1.0 N J:THR890 4.9 91.2 1.0 C J:VAL894 5.0 82.2 1.0

P.K.Zuber, N.Said, T.Hilal, B.Wang, B.Loll, J.Gonzalez-Higueras, C.A.Ramirez-Sarmiento, G.A.Belogurov, I.Artsimovitch, M.C.Wahl, S.H.Knauer. Concerted Transformation of A Hyper-Paused Transcription Complex and Its Reinforcing Protein. Nat Commun V. 15 3040 2024.
ISSN: ESSN 2041-1723
PubMed: 38589445
DOI: 10.1038/S41467-024-47368-4