Zinc in PDB 8pbr: Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form

All present enzymatic activity of Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form:
2.1.3.2; 3.5.2.3; 6.3.5.5;

The structure of Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form, PDB code: 8pbr was solved by F.Del Cano-Ochoa, S.Ramon-Maiques, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.45 / 2.06
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	81.738, 159.318, 61.031, 90, 90, 90
R / Rfree (%)	16.8 / 22.1

The binding sites of Zinc atom in the Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form (pdb code 8pbr). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form, PDB code: 8pbr:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in the Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1901 b:32.9 occ:1.00 NE2 A:HIS1471 2.0 26.7 1.0 OQ2 A:KCX1556 2.1 37.6 1.0 O A:HOH2025 2.1 35.2 1.0 NE2 A:HIS1473 2.2 30.8 1.0 OD1 A:ASP1686 2.4 33.4 1.0 CD2 A:HIS1471 2.9 26.6 1.0 CE1 A:HIS1471 3.0 28.9 1.0 CX A:KCX1556 3.1 37.4 1.0 CE1 A:HIS1473 3.1 31.2 1.0 HD2 A:HIS1471 3.1 31.9 1.0 HE1 A:HIS1473 3.2 37.6 1.0 CD2 A:HIS1473 3.2 31.2 1.0 ZN A:ZN1902 3.2 34.7 1.0 CG A:ASP1686 3.2 35.9 1.0 HE1 A:HIS1471 3.3 34.8 1.0 OQ1 A:KCX1556 3.4 37.7 1.0 HD2 A:HIS1473 3.4 37.6 1.0 HG3 A:MET1503 3.5 39.3 1.0 OD2 A:ASP1686 3.5 37.2 1.0 HD2 A:HIS1614 3.9 39.9 1.0 CG A:HIS1471 4.1 27.3 1.0 ND1 A:HIS1471 4.1 28.7 1.0 NZ A:KCX1556 4.2 35.3 1.0 ND1 A:HIS1473 4.2 31.0 1.0 HA A:ASP1686 4.2 40.8 1.0 CG A:HIS1473 4.3 31.0 1.0 HH A:TYR1558 4.3 27.6 0.6 NE2 A:HIS1614 4.4 34.0 1.0 HE2 A:TYR1558 4.4 31.5 0.6 HZ A:KCX1556 4.4 42.5 1.0 O A:HOH2152 4.4 53.5 1.0 CD2 A:HIS1614 4.4 33.2 1.0 CG A:MET1503 4.5 32.6 1.0 CB A:ASP1686 4.5 35.2 1.0 HE3 A:MET1503 4.6 41.0 1.0 HB2 A:ASP1686 4.7 42.3 1.0 CA A:ASP1686 4.8 33.9 1.0 HD1 A:HIS1471 4.9 34.5 1.0 HG2 A:MET1503 4.9 39.3 1.0 HD22 A:ASN1505 4.9 47.2 1.0 HE2 A:TYR1558 4.9 33.3 0.5 HD1 A:HIS1473 5.0 37.3 1.0

Zinc binding site 2 out of 3 in the Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1902 b:34.7 occ:1.00 OQ1 A:KCX1556 1.9 37.7 1.0 NE2 A:HIS1614 2.1 34.0 1.0 ND1 A:HIS1590 2.1 30.7 1.0 O A:HOH2025 2.2 35.2 1.0 CX A:KCX1556 2.8 37.4 1.0 HB2 A:HIS1590 2.9 33.9 1.0 CE1 A:HIS1614 3.0 34.8 1.0 CE1 A:HIS1590 3.0 32.1 1.0 OQ2 A:KCX1556 3.0 37.6 1.0 HE1 A:HIS1471 3.1 34.8 1.0 CD2 A:HIS1614 3.1 33.2 1.0 HE1 A:HIS1590 3.1 38.6 1.0 HE1 A:HIS1614 3.1 41.8 1.0 CG A:HIS1590 3.1 29.5 1.0 ZN A:ZN1901 3.2 32.9 1.0 HD2 A:HIS1614 3.3 39.9 1.0 CB A:HIS1590 3.5 28.1 1.0 CE1 A:HIS1471 3.7 28.9 1.0 HE2 A:TYR1558 3.8 33.3 0.5 NE2 A:HIS1471 3.9 26.7 1.0 NZ A:KCX1556 4.0 35.3 1.0 ND1 A:HIS1614 4.1 33.9 1.0 HB3 A:CYS1613 4.1 43.6 1.0 NE2 A:HIS1590 4.1 32.5 1.0 HE2 A:TYR1558 4.1 31.5 0.6 HA A:HIS1590 4.2 33.3 1.0 CG A:HIS1614 4.2 33.4 1.0 CE2 A:TYR1558 4.2 27.7 0.5 HB3 A:HIS1590 4.2 33.9 1.0 CD2 A:HIS1590 4.2 31.3 1.0 OD2 A:ASP1686 4.2 37.2 1.0 HE2 A:KCX1556 4.3 39.2 1.0 HD3 A:PRO1662 4.3 53.4 1.0 HB2 A:CYS1613 4.4 43.6 1.0 HH A:TYR1558 4.4 34.0 0.5 CA A:HIS1590 4.5 27.7 1.0 CE A:KCX1556 4.6 32.6 1.0 O A:ARG1661 4.6 45.9 1.0 HE3 A:KCX1556 4.6 39.2 1.0 CB A:CYS1613 4.7 36.2 1.0 HD2 A:TYR1558 4.7 31.9 0.6 HZ A:KCX1556 4.7 42.5 1.0 CZ A:TYR1558 4.7 27.4 0.5 CE2 A:TYR1558 4.7 26.2 0.6 OH A:TYR1558 4.8 28.2 0.5 OD1 A:ASP1686 4.8 33.4 1.0 CD2 A:TYR1558 4.8 27.3 0.5 CG A:ASP1686 4.8 35.9 1.0 HD2 A:TYR1558 4.8 32.9 0.5 HE2 A:HIS1590 4.9 39.1 1.0 HD1 A:HIS1614 4.9 40.8 1.0 O A:HOH2152 5.0 53.5 1.0 ND1 A:HIS1471 5.0 28.7 1.0

Zinc binding site 3 out of 3 in the Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Mutant R1475Q of the Dihydroorotase Domain of Human Cad Protein in Apo Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn1903 b:42.4 occ:0.35 O A:GLY1806 2.2 27.8 1.0 O A:HOH2141 2.3 46.4 1.0 O A:GLY1804 2.3 34.8 1.0 O A:GLU1524 2.6 34.9 1.0 O A:HOH2107 2.8 46.0 1.0 O A:HOH2135 3.0 44.8 1.0 C A:GLY1804 3.3 33.7 1.0 HA A:GLU1524 3.3 43.2 1.0 C A:GLY1806 3.4 29.3 1.0 HA2 A:GLY1804 3.4 41.3 1.0 C A:GLU1524 3.5 34.8 1.0 HA A:GLN1807 3.6 35.1 1.0 HB3 A:GLU1524 3.7 44.8 1.0 HG2 A:GLN1807 3.8 38.5 1.0 CA A:GLU1524 3.8 36.0 1.0 CA A:GLY1804 3.8 34.3 1.0 N A:GLY1806 4.0 30.3 1.0 C A:TYR1805 4.0 31.1 1.0 H A:GLY1806 4.1 36.5 1.0 HA3 A:GLY1804 4.2 41.3 1.0 CB A:GLU1524 4.2 37.3 1.0 HG2 A:GLU1524 4.2 46.5 1.0 CA A:GLY1806 4.3 30.0 1.0 N A:GLN1807 4.3 30.1 1.0 N A:TYR1805 4.3 32.5 1.0 O A:TYR1805 4.3 30.7 1.0 CA A:GLN1807 4.4 29.2 1.0 HG3 A:GLN1807 4.4 38.5 1.0 HA A:TYR1805 4.5 39.5 1.0 CG A:GLN1807 4.5 32.0 1.0 CA A:TYR1805 4.5 32.8 1.0 HE2 A:PHE1531 4.6 36.6 1.0 N A:ALA1525 4.8 35.6 1.0 HA3 A:GLY1806 4.8 36.0 1.0 HA A:ALA1525 4.8 43.8 1.0 CG A:GLU1524 4.8 38.6 1.0 HA2 A:GLY1806 5.0 36.0 1.0 HB3 A:ALA1527 5.0 39.5 1.0 O A:HOH2051 5.0 35.7 1.0 O1 A:FMT1906 5.0 57.8 1.0

F.Del Cano-Ochoa, B.G.Ng, A.Rubio-Del-Campo, S.Mahajan, M.P.Wilson, M.Vilar, D.Rymen, P.Sanchez-Pintos, J.Kenny, M.Ley Martos, T.Campos, S.B.Wortmann, H.H.Freeze, S.Ramon-Maiques. Beyond Genetics: Deciphering the Impact of Missense Variants in Cad Deficiency. J Inherit Metab Dis 2023.
ISSN: ISSN 1573-2665
PubMed: 37540500
DOI: 10.1002/JIMD.12667