Zinc in PDB 8gip: Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+

All present enzymatic activity of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+:
2.7.7.86;

The structure of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+, PDB code: 8gip was solved by S.Wu, J.Sohn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.79 / 2.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	77.933, 98.397, 142.47, 90, 90, 90
R / Rfree (%)	18.6 / 24.2

The structure of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+ also contains other interesting chemical elements:

Manganese	(Mn)	2 atoms
Magnesium	(Mg)	2 atoms

The binding sites of Zinc atom in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+ (pdb code 8gip). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+, PDB code: 8gip:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn603 b:37.9 occ:1.00 NE2 A:HIS378 2.1 38.8 1.0 SG A:CYS385 2.3 39.5 1.0 SG A:CYS392 2.3 38.5 1.0 SG A:CYS384 2.3 38.7 1.0 CD2 A:HIS378 2.9 34.3 1.0 CE1 A:HIS378 3.2 32.9 1.0 CB A:CYS392 3.2 38.4 1.0 CB A:CYS385 3.4 34.3 1.0 CB A:CYS384 3.7 30.9 1.0 N A:CYS392 3.7 37.8 1.0 N A:CYS385 3.9 37.8 1.0 C A:CYS384 3.9 36.4 1.0 CA A:CYS392 4.0 37.8 1.0 O A:HOH710 4.1 38.7 1.0 CG A:HIS378 4.1 35.9 1.0 CA A:CYS385 4.2 34.5 1.0 ND1 A:HIS378 4.2 34.4 1.0 O A:CYS384 4.2 37.3 1.0 CA A:CYS384 4.3 34.2 1.0 NH1 A:ARG394 4.4 36.5 1.0 C A:CYS392 4.5 40.9 1.0 O A:CYS392 4.5 43.4 1.0 C A:LYS391 4.8 45.3 1.0 O A:ALA390 4.9 44.0 1.0 O A:HOH711 5.0 33.1 1.0

Zinc binding site 2 out of 2 in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.040MM MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn603 b:41.3 occ:1.00 NE2 C:HIS378 2.1 38.0 1.0 SG C:CYS385 2.3 39.2 1.0 SG C:CYS384 2.3 37.4 1.0 SG C:CYS392 2.3 39.7 1.0 CD2 C:HIS378 3.0 33.5 1.0 CE1 C:HIS378 3.1 32.3 1.0 CB C:CYS392 3.3 38.9 1.0 CB C:CYS385 3.3 34.1 1.0 CB C:CYS384 3.6 37.6 1.0 N C:CYS392 3.7 35.6 1.0 N C:CYS385 3.8 40.4 1.0 O C:HOH715 3.8 37.9 1.0 C C:CYS384 3.8 37.5 1.0 CA C:CYS392 4.0 39.5 1.0 CA C:CYS385 4.1 35.4 1.0 CG C:HIS378 4.2 36.0 1.0 ND1 C:HIS378 4.2 33.8 1.0 CA C:CYS384 4.2 35.6 1.0 O C:CYS384 4.3 36.8 1.0 NH1 C:ARG394 4.3 39.2 1.0 O C:CYS392 4.5 44.0 1.0 C C:CYS392 4.5 40.1 1.0 O C:HOH717 4.6 38.4 1.0 O C:ALA390 4.8 39.8 1.0 C C:LYS391 4.8 39.1 1.0

S.Wu, S.B.Gabelli, J.Sohn. The Structural Basis For 2'-5'/3'-5'-Cgamp Synthesis By Cgas Nat Commun V. 15 4012 2024.
ISSN: ESSN 2041-1723
DOI: 10.1038/S41467-024-48365-3