Zinc in PDB 8gin: Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+

All present enzymatic activity of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+:
2.7.7.86;

The structure of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+, PDB code: 8gin was solved by S.Wu, J.Sohn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.78 / 2.75
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	77.392, 98.709, 142.26, 90, 90, 90
R / Rfree (%)	18.1 / 22.5

The structure of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+ also contains other interesting chemical elements:

Magnesium	(Mg)	2 atoms
Manganese	(Mn)	2 atoms

The binding sites of Zinc atom in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+ (pdb code 8gin). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+, PDB code: 8gin:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn602 b:46.6 occ:1.00 NE2 A:HIS378 2.0 45.9 1.0 SG A:CYS385 2.3 50.0 1.0 SG A:CYS392 2.3 46.1 1.0 SG A:CYS384 2.4 44.3 1.0 CD2 A:HIS378 2.8 42.2 1.0 CE1 A:HIS378 3.1 42.2 1.0 CB A:CYS392 3.2 45.4 1.0 CB A:CYS385 3.4 43.5 1.0 CB A:CYS384 3.6 38.6 1.0 N A:CYS392 3.8 43.8 1.0 N A:CYS385 3.9 40.7 1.0 C A:CYS384 3.9 41.3 1.0 CA A:CYS392 4.0 44.1 1.0 CG A:HIS378 4.0 44.1 1.0 ND1 A:HIS378 4.1 41.5 1.0 CA A:CYS385 4.2 40.7 1.0 O A:CYS384 4.2 42.9 1.0 CA A:CYS384 4.3 39.1 1.0 NH1 A:ARG394 4.4 40.7 1.0 C A:CYS392 4.6 45.0 1.0 O A:CYS392 4.8 50.7 1.0 O A:ALA390 4.9 46.8 1.0 C A:LYS391 4.9 48.5 1.0

Zinc binding site 2 out of 2 in the Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Ternary Complex of Mouse Cgas with Dsdna and Bound Atp: with 10MM MG2+ and 0.015MM MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn602 b:49.6 occ:1.00 NE2 C:HIS378 2.1 49.1 1.0 SG C:CYS385 2.3 51.7 1.0 SG C:CYS384 2.3 50.6 1.0 SG C:CYS392 2.4 49.5 1.0 CD2 C:HIS378 3.0 46.2 1.0 CE1 C:HIS378 3.2 45.3 1.0 CB C:CYS385 3.3 48.4 1.0 CB C:CYS392 3.4 53.1 1.0 O C:HOH710 3.6 46.6 1.0 N C:CYS392 3.7 48.4 1.0 CB C:CYS384 3.7 45.4 1.0 N C:CYS385 3.9 49.8 1.0 C C:CYS384 3.9 47.0 1.0 CA C:CYS392 4.0 51.1 1.0 CA C:CYS385 4.1 49.2 1.0 CG C:HIS378 4.2 46.8 1.0 ND1 C:HIS378 4.3 45.2 1.0 O C:CYS384 4.3 46.3 1.0 CA C:CYS384 4.4 44.9 1.0 NH1 C:ARG394 4.4 48.5 1.0 C C:CYS392 4.5 50.9 1.0 O C:CYS392 4.6 52.1 1.0 O C:ALA390 4.8 55.9 1.0 C C:LYS391 4.8 50.0 1.0

S.Wu, S.B.Gabelli, J.Sohn. The Structural Basis For 2'-5'/3'-5'-Cgamp Synthesis By Cgas Nat Commun V. 15 4012 2024.
ISSN: ESSN 2041-1723
DOI: 10.1038/S41467-024-48365-3