Zinc in PDB 8bd5: CAS12K-Sgrna-Dsdna-S15-Tniq-Tnsc Transposon Recruitment Complex

The structure of CAS12K-Sgrna-Dsdna-S15-Tniq-Tnsc Transposon Recruitment Complex also contains other interesting chemical elements:

Magnesium

(Mg)

7 atoms

The binding sites of Zinc atom in the CAS12K-Sgrna-Dsdna-S15-Tniq-Tnsc Transposon Recruitment Complex (pdb code 8bd5). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the CAS12K-Sgrna-Dsdna-S15-Tniq-Tnsc Transposon Recruitment Complex, PDB code: 8bd5:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the CAS12K-Sgrna-Dsdna-S15-Tniq-Tnsc Transposon Recruitment Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of CAS12K-Sgrna-Dsdna-S15-Tniq-Tnsc Transposon Recruitment Complex within 5.0Å range: probe atom residue distance (Å) B Occ Q:Zn201 b:93.7 occ:1.00 SG Q:CYS154 2.3 81.8 1.0 SG Q:CYS136 2.3 77.1 1.0 SG Q:CYS151 2.3 78.4 1.0 SG Q:CYS133 2.3 71.2 1.0 OD1 Q:ASN135 3.0 76.1 1.0 CB Q:CYS151 3.0 78.4 1.0 CB Q:CYS133 3.1 71.2 1.0 CB Q:CYS154 3.1 81.8 1.0 CB Q:CYS136 3.8 77.1 1.0 N Q:CYS154 3.9 81.8 1.0 CG Q:ASN135 3.9 76.1 1.0 ND2 Q:ASN135 4.0 76.1 1.0 CA Q:CYS154 4.1 81.8 1.0 N Q:CYS136 4.3 77.1 1.0 CB Q:ALA138 4.3 68.9 1.0 CA Q:CYS151 4.5 78.4 1.0 CA Q:CYS136 4.5 77.1 1.0 CA Q:CYS133 4.6 71.2 1.0 C Q:ARG153 4.8 78.0 1.0 C Q:CYS154 4.8 81.8 1.0 C Q:CYS151 4.9 78.4 1.0 CB Q:ARG153 4.9 78.0 1.0 N Q:ALA138 4.9 68.9 1.0 CE Q:MET161 4.9 76.1 1.0 C Q:CYS136 5.0 77.1 1.0 N Q:ARG153 5.0 78.0 1.0

Zinc binding site 2 out of 2 in the CAS12K-Sgrna-Dsdna-S15-Tniq-Tnsc Transposon Recruitment Complex


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of CAS12K-Sgrna-Dsdna-S15-Tniq-Tnsc Transposon Recruitment Complex within 5.0Å range: probe atom residue distance (Å) B Occ Q:Zn202 b:96.1 occ:1.00 ND1 Q:HIS125 2.0 82.3 1.0 SG Q:CYS103 2.3 77.9 1.0 SG Q:CYS100 2.3 74.1 1.0 SG Q:CYS122 2.3 82.8 1.0 CE1 Q:HIS125 2.7 82.3 1.0 CB Q:CYS103 3.0 77.9 1.0 CG Q:HIS125 3.1 82.3 1.0 N Q:CYS103 3.2 77.9 1.0 CB Q:CYS122 3.5 82.8 1.0 CB Q:CYS100 3.6 74.1 1.0 CA Q:CYS103 3.6 77.9 1.0 CB Q:HIS125 3.6 82.3 1.0 NE2 Q:HIS125 3.8 82.3 1.0 CD2 Q:HIS125 4.0 82.3 1.0 CB Q:ALA102 4.1 77.0 1.0 C Q:ALA102 4.1 77.0 1.0 CA Q:ALA102 4.5 77.0 1.0 N Q:ALA102 4.6 77.0 1.0 CG Q:ARG124 4.8 85.5 1.0 CA Q:CYS100 4.8 74.1 1.0 CA Q:CYS122 4.9 82.8 1.0 N Q:HIS125 4.9 82.3 1.0 CA Q:HIS125 4.9 82.3 1.0 C Q:CYS100 4.9 74.1 1.0 O Q:CYS100 5.0 74.1 1.0

M.Schmitz, I.Querques, S.Oberli, C.Chanez, M.Jinek. Structural Basis For the Assembly of the Type V Crispr-Associated Transposon Complex. Cell V. 185 4999 2022.
ISSN: ISSN 1097-4172
PubMed: 36435179
DOI: 10.1016/J.CELL.2022.11.009