Zinc in PDB 7v8f: Crystal Structure of UBE2L3 Bound to Hoip RING1 Domain.

Enzymatic activity of Crystal Structure of UBE2L3 Bound to Hoip RING1 Domain.

All present enzymatic activity of Crystal Structure of UBE2L3 Bound to Hoip RING1 Domain.:
2.3.2.23; 2.3.2.31;

Protein crystallography data

The structure of Crystal Structure of UBE2L3 Bound to Hoip RING1 Domain., PDB code: 7v8f was solved by J.Liu, Y.Wang, L.Pan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 54.21 / 1.66
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 30.097, 108.412, 37.156, 90, 106.33, 90
R / Rfree (%) 17.5 / 19.8

Zinc Binding Sites:

The binding sites of Zinc atom in the Crystal Structure of UBE2L3 Bound to Hoip RING1 Domain. (pdb code 7v8f). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of UBE2L3 Bound to Hoip RING1 Domain., PDB code: 7v8f:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 7v8f

Go back to Zinc Binding Sites List in 7v8f
Zinc binding site 1 out of 2 in the Crystal Structure of UBE2L3 Bound to Hoip RING1 Domain.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of UBE2L3 Bound to Hoip RING1 Domain. within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn801

b:23.4
occ:1.00
SG B:CYS719 2.3 24.8 1.0
SG B:CYS747 2.3 24.3 1.0
SG B:CYS744 2.3 18.1 1.0
SG B:CYS717 2.4 26.4 1.0
CB B:CYS719 3.1 22.7 1.0
CB B:CYS744 3.1 18.1 1.0
CB B:CYS747 3.3 23.0 1.0
CB B:CYS717 3.4 31.0 1.0
N B:CYS747 3.7 20.5 1.0
O B:HOH985 4.0 42.0 1.0
CA B:CYS747 4.1 22.0 1.0
O B:HOH950 4.1 40.8 1.0
N B:CYS719 4.1 27.3 1.0
CA B:CYS719 4.2 28.7 1.0
CA B:CYS744 4.6 20.2 1.0
C B:ALA746 4.7 21.8 1.0
CB B:ALA746 4.7 22.8 1.0
O B:HOH964 4.7 31.1 1.0
CA B:CYS717 4.7 28.3 1.0
C B:CYS747 4.8 24.8 1.0
N B:GLU718 5.0 26.4 1.0
N B:GLY748 5.0 23.1 1.0
N B:ALA746 5.0 19.3 1.0

Zinc binding site 2 out of 2 in 7v8f

Go back to Zinc Binding Sites List in 7v8f
Zinc binding site 2 out of 2 in the Crystal Structure of UBE2L3 Bound to Hoip RING1 Domain.


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of UBE2L3 Bound to Hoip RING1 Domain. within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn802

b:21.2
occ:1.00
SG B:CYS702 2.3 20.4 1.0
SG B:CYS725 2.3 19.6 1.0
SG B:CYS699 2.4 20.1 1.0
SG B:CYS722 2.4 21.6 1.0
CB B:CYS725 3.1 21.6 1.0
CB B:CYS699 3.2 18.7 1.0
CB B:CYS702 3.2 23.7 1.0
CB B:CYS722 3.5 20.6 1.0
N B:CYS702 3.7 20.6 1.0
NH2 A:ARG5 4.0 23.4 1.0
N B:CYS722 4.0 19.4 1.0
CA B:CYS702 4.1 18.2 1.0
NE A:ARG5 4.1 22.4 1.0
CA B:CYS722 4.3 23.4 1.0
CA B:CYS725 4.4 21.4 1.0
N B:CYS725 4.4 21.4 1.0
CB B:VAL701 4.4 16.8 1.0
CZ A:ARG5 4.5 22.0 1.0
CA B:CYS699 4.6 18.8 1.0
C B:VAL701 4.8 21.8 1.0
C B:CYS702 4.9 20.1 1.0
O B:CYS722 4.9 20.3 1.0
C B:CYS722 5.0 23.1 1.0
CA B:VAL701 5.0 18.6 1.0

Reference:

J.Liu, Y.Wang, D.Wang, Y.Wang, X.Xu, Y.Zhang, Y.Li, M.Zhang, X.Gong, Y.Tang, L.Shen, M.Li, L.Pan. Mechanistic Insights Into the Subversion of the Linear Ubiquitin Chain Assembly Complex By the E3 Ligase IPAH1.4 of Shigella Flexneri. Proc.Natl.Acad.Sci.Usa V. 119 76119 2022.
ISSN: ESSN 1091-6490
PubMed: 35294289
DOI: 10.1073/PNAS.2116776119
Page generated: Sat Apr 8 04:40:38 2023

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