Zinc in PDB 7bbd: Crystal Structure of Monoubiquitinated TRIM21 Ring (Ub-Ring) in Complex with Ubiquitin Charged UBE2N (UBE2N~Ub) and UBE2V2

All present enzymatic activity of Crystal Structure of Monoubiquitinated TRIM21 Ring (Ub-Ring) in Complex with Ubiquitin Charged UBE2N (UBE2N~Ub) and UBE2V2:
2.3.2.23; 2.3.2.27;

The structure of Crystal Structure of Monoubiquitinated TRIM21 Ring (Ub-Ring) in Complex with Ubiquitin Charged UBE2N (UBE2N~Ub) and UBE2V2, PDB code: 7bbd was solved by L.Kiss, D.Neuhaus, L.C.James, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.99 / 2.20
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	99.15, 108.36, 75.14, 90, 104.99, 90
R / Rfree (%)	22.2 / 25.2

The binding sites of Zinc atom in the Crystal Structure of Monoubiquitinated TRIM21 Ring (Ub-Ring) in Complex with Ubiquitin Charged UBE2N (UBE2N~Ub) and UBE2V2 (pdb code 7bbd). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of Monoubiquitinated TRIM21 Ring (Ub-Ring) in Complex with Ubiquitin Charged UBE2N (UBE2N~Ub) and UBE2V2, PDB code: 7bbd:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of Monoubiquitinated TRIM21 Ring (Ub-Ring) in Complex with Ubiquitin Charged UBE2N (UBE2N~Ub) and UBE2V2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Monoubiquitinated TRIM21 Ring (Ub-Ring) in Complex with Ubiquitin Charged UBE2N (UBE2N~Ub) and UBE2V2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn201 b:26.4 occ:1.00 SG B:CYS95 2.3 24.7 1.0 SG B:CYS115 2.3 27.9 1.0 SG B:CYS92 2.4 18.4 1.0 SG B:CYS112 2.4 26.9 1.0 CB B:CYS112 2.9 20.9 1.0 CB B:CYS92 2.9 18.1 1.0 CB B:CYS95 3.2 22.0 1.0 CB B:CYS115 3.3 27.0 1.0 N B:CYS95 3.7 18.7 1.0 N B:CYS112 3.9 22.2 1.0 CA B:CYS112 4.0 21.4 1.0 CA B:CYS95 4.0 23.3 1.0 CA B:CYS92 4.4 17.7 1.0 CB B:ILE94 4.6 26.2 1.0 CA B:CYS115 4.7 26.0 1.0 C B:ILE94 4.8 22.4 1.0 C B:CYS95 4.9 21.3 1.0 C B:CYS92 4.9 18.5 1.0 N B:ILE94 5.0 17.9 1.0 C B:CYS112 5.0 22.8 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of Monoubiquitinated TRIM21 Ring (Ub-Ring) in Complex with Ubiquitin Charged UBE2N (UBE2N~Ub) and UBE2V2


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Monoubiquitinated TRIM21 Ring (Ub-Ring) in Complex with Ubiquitin Charged UBE2N (UBE2N~Ub) and UBE2V2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn202 b:20.8 occ:1.00 ND1 B:HIS109 2.0 17.1 1.0 SG B:CYS107 2.3 24.4 1.0 SG B:CYS127 2.3 19.3 1.0 SG B:CYS130 2.3 24.7 1.0 CE1 B:HIS109 2.8 26.5 1.0 CB B:CYS127 3.1 18.2 1.0 CG B:HIS109 3.2 25.4 1.0 CB B:CYS107 3.2 26.8 1.0 CB B:CYS130 3.4 23.2 1.0 CB B:HIS109 3.7 20.7 1.0 N B:CYS130 3.7 22.4 1.0 NE2 B:HIS109 4.0 27.3 1.0 CA B:CYS130 4.1 29.8 1.0 CD2 B:HIS109 4.2 21.8 1.0 CB B:VAL129 4.5 23.9 1.0 CD1 B:ILE105 4.6 19.9 1.0 CA B:CYS127 4.6 18.4 1.0 N B:HIS109 4.6 21.8 1.0 CA B:CYS107 4.6 28.1 1.0 C B:VAL129 4.6 23.8 1.0 C B:CYS130 4.7 24.8 1.0 CA B:HIS109 4.8 21.5 1.0 N B:ARG131 4.8 27.3 1.0 O B:HOH343 4.8 34.4 1.0 C B:CYS107 4.9 25.8 1.0 N B:VAL129 4.9 19.1 1.0 CB B:GLN132 4.9 31.2 1.0 CA B:VAL129 4.9 23.1 1.0 N B:GLN132 5.0 30.8 1.0

L.Kiss, D.Clift, D.Neuhaus, L.C.James. Ring Domains Act As Both Substrate and Enzyme in A Catalytic Arrangement to Drive Self-Anchored Ubiquitination To Be Published.