Zinc in PDB 7a3x: Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Sulfolane

All present enzymatic activity of Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Sulfolane:
2.4.2.29;

The structure of Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Sulfolane, PDB code: 7a3x was solved by D.Nguyen, A.Heine, G.Klebe, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.79 / 1.85
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	128.292, 128.292, 114.859, 90, 90, 120
R / Rfree (%)	20.3 / 22.6

The binding sites of Zinc atom in the Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Sulfolane (pdb code 7a3x). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total only one binding site of Zinc was determined in the Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Sulfolane, PDB code: 7a3x:

Zinc binding site 1 out of 1 in the Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Sulfolane


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Trna-Guanine Transglycosylase C158S/C281S/Y330C/H333A Mutant in Complex with Sulfolane within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn401 b:25.4 occ:1.00 ND1 A:HIS349 2.1 27.2 1.0 SG A:CYS320 2.3 26.0 1.0 SG A:CYS323 2.3 28.5 1.0 SG A:CYS318 2.3 27.1 1.0 CE1 A:HIS349 3.0 29.3 1.0 CG A:HIS349 3.2 26.9 1.0 CB A:CYS323 3.3 28.1 1.0 CB A:CYS318 3.3 24.7 1.0 CB A:CYS320 3.3 28.0 1.0 CB A:HIS349 3.7 22.5 1.0 N A:CYS323 3.9 26.0 1.0 N A:CYS320 4.1 30.9 1.0 CA A:HIS349 4.1 20.9 1.0 NE2 A:HIS349 4.2 25.4 1.0 CA A:CYS323 4.2 25.8 1.0 CA A:CYS320 4.2 26.3 1.0 CD2 A:HIS349 4.3 21.7 1.0 O A:HIS349 4.5 26.0 1.0 CA A:CYS318 4.6 24.0 1.0 C A:CYS318 4.6 27.0 1.0 O A:CYS320 4.7 27.6 1.0 C A:CYS320 4.7 29.2 1.0 C A:HIS349 4.7 19.1 1.0 O A:CYS318 4.7 26.3 1.0 CB A:VAL322 4.8 22.6 1.0 C A:VAL322 4.9 26.2 1.0

D.Nguyen, X.Xie, S.Jakobi, F.Terwesten, A.Metz, T.X.P.Nguyen, V.A.Palchykov, A.Heine, K.Reuter, G.Klebe. Targeting A Cryptic Pocket in A Protein-Protein Contact By Disulfide-Induced Rupture of A Homodimeric Interface Acs Chem.Biol. 2021.
ISSN: ESSN 1554-8937
DOI: 10.1021/ACSCHEMBIO.1C00296