Zinc in PDB 6grh: E. Coli Microcin Synthetase Mcbbcd Complex with Truncated Pro-MCCB17 Bound

The structure of E. Coli Microcin Synthetase Mcbbcd Complex with Truncated Pro-MCCB17 Bound, PDB code: 6grh was solved by D.Ghilarov, C.E.M.Stevenson, D.Y.Travin, J.Piskunova, M.Serebryakova, A.Maxwell, D.M.Lawson, K.Severinov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	57.54 / 1.85
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	180.740, 83.760, 87.120, 90.00, 91.33, 90.00
R / Rfree (%)	17.2 / 20.7

The structure of E. Coli Microcin Synthetase Mcbbcd Complex with Truncated Pro-MCCB17 Bound also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the E. Coli Microcin Synthetase Mcbbcd Complex with Truncated Pro-MCCB17 Bound (pdb code 6grh). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the E. Coli Microcin Synthetase Mcbbcd Complex with Truncated Pro-MCCB17 Bound, PDB code: 6grh:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the E. Coli Microcin Synthetase Mcbbcd Complex with Truncated Pro-MCCB17 Bound


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of E. Coli Microcin Synthetase Mcbbcd Complex with Truncated Pro-MCCB17 Bound within 5.0Å range: probe atom residue distance (Å) B Occ 1:Zn401 b:26.0 occ:1.00 SG 1:CYS290 2.3 30.0 1.0 SG 1:CYS292 2.3 31.7 1.0 SG 1:CYS192 2.3 27.0 1.0 SG 1:CYS195 2.3 26.7 1.0 CB 1:CYS292 3.3 33.1 1.0 CB 1:CYS290 3.3 30.7 1.0 CB 1:CYS192 3.4 27.0 1.0 CB 1:CYS195 3.6 26.1 1.0 N 1:CYS195 3.6 24.8 1.0 N 1:CYS192 3.6 25.2 1.0 CA 1:CYS192 4.0 25.3 1.0 CA 1:CYS195 4.1 25.3 1.0 O 1:HOH595 4.2 52.0 1.0 N 1:CYS292 4.3 33.9 1.0 CA 1:CYS292 4.4 34.3 1.0 C 1:CYS192 4.4 23.9 1.0 O 1:CYS192 4.4 23.9 1.0 O 1:HOH526 4.5 35.4 1.0 C 1:PHE194 4.5 24.7 1.0 CA 1:CYS290 4.7 30.4 1.0 CB 1:PHE194 4.7 25.9 1.0 C 1:PRO191 4.7 25.3 1.0 N 1:PHE194 4.7 24.5 1.0 CA 1:PHE194 4.9 25.0 1.0 CA 1:PRO191 4.9 25.5 1.0

Zinc binding site 2 out of 2 in the E. Coli Microcin Synthetase Mcbbcd Complex with Truncated Pro-MCCB17 Bound


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of E. Coli Microcin Synthetase Mcbbcd Complex with Truncated Pro-MCCB17 Bound within 5.0Å range: probe atom residue distance (Å) B Occ 2:Zn401 b:28.0 occ:1.00 SG 2:CYS292 2.3 32.2 1.0 SG 2:CYS192 2.3 28.0 1.0 SG 2:CYS290 2.3 33.0 1.0 SG 2:CYS195 2.3 27.9 1.0 CB 2:CYS290 3.2 33.1 1.0 CB 2:CYS292 3.3 33.5 1.0 CB 2:CYS192 3.4 27.9 1.0 N 2:CYS195 3.5 27.8 1.0 CB 2:CYS195 3.5 28.3 1.0 N 2:CYS192 3.6 26.7 1.0 CA 2:CYS195 3.9 27.2 1.0 CA 2:CYS192 3.9 26.9 1.0 N 2:CYS292 4.2 32.9 1.0 CA 2:CYS292 4.3 33.9 1.0 O 2:CYS192 4.3 25.9 1.0 C 2:CYS192 4.4 26.4 1.0 C 2:PHE194 4.4 27.2 1.0 CA 2:CYS290 4.6 32.8 1.0 C 2:PRO191 4.7 27.0 1.0 N 2:PHE194 4.7 26.3 1.0 CB 2:PHE194 4.7 28.7 1.0 CA 2:PHE194 4.8 27.6 1.0 ND1 2:HIS286 4.8 33.3 1.0 C 2:CYS290 4.9 33.0 1.0 CA 2:PRO191 4.9 27.1 1.0

D.Ghilarov, C.E.M.Stevenson, D.Y.Travin, J.Piskunova, M.Serebryakova, A.Maxwell, D.M.Lawson, K.Severinov. Architecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting A Ribosomally Synthesized Peptide Into A Dna Gyrase Poison. Mol. Cell V. 73 749 2019.
ISSN: ISSN 1097-4164
PubMed: 30661981
DOI: 10.1016/J.MOLCEL.2018.11.032