Zinc in PDB 6gos: E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound

The structure of E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound, PDB code: 6gos was solved by D.Ghilarov, C.E.M.Stevenson, D.Y.Travin, J.Piskunova, M.Serebryakova, A.Maxwell, D.M.Lawson, K.Severinov, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	57.39 / 2.10
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	180.960, 83.400, 86.900, 90.00, 91.45, 90.00
R / Rfree (%)	17.2 / 21.2

The structure of E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Zinc atom in the E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound (pdb code 6gos). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound, PDB code: 6gos:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound within 5.0Å range: probe atom residue distance (Å) B Occ 1:Zn301 b:31.2 occ:1.00 SG 1:CYS192 2.3 29.6 1.0 SG 1:CYS195 2.3 29.2 1.0 SG 1:CYS292 2.3 36.1 1.0 SG 1:CYS290 2.3 35.0 1.0 CB 1:CYS292 3.2 38.9 1.0 CB 1:CYS290 3.4 34.7 1.0 CB 1:CYS192 3.4 31.1 1.0 CB 1:CYS195 3.5 31.7 1.0 N 1:CYS192 3.6 30.9 1.0 N 1:CYS195 3.6 27.1 1.0 CA 1:CYS192 3.9 30.2 1.0 CA 1:CYS195 4.0 29.0 1.0 N 1:CYS292 4.2 38.8 1.0 O 1:HOH425 4.3 34.8 1.0 CA 1:CYS292 4.3 38.9 1.0 C 1:CYS192 4.4 27.2 1.0 O 1:CYS192 4.5 27.7 1.0 C 1:PHE194 4.5 27.5 1.0 C 1:PRO191 4.7 30.7 1.0 N 1:PHE194 4.7 30.1 1.0 CA 1:CYS290 4.7 36.2 1.0 CB 1:PHE194 4.7 30.8 1.0 CA 1:PHE194 4.9 29.5 1.0 CA 1:PRO191 4.9 31.7 1.0

Zinc binding site 2 out of 2 in the E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of E. Coli Microcin Synthetase Mcbbcd Complex with Pro-MCCB17 Bound within 5.0Å range: probe atom residue distance (Å) B Occ 2:Zn401 b:34.0 occ:1.00 SG 2:CYS292 2.3 37.5 1.0 SG 2:CYS192 2.3 34.9 1.0 SG 2:CYS195 2.3 32.6 1.0 SG 2:CYS290 2.3 40.1 1.0 CB 2:CYS290 3.2 40.0 1.0 CB 2:CYS292 3.3 40.0 1.0 N 2:CYS195 3.4 33.6 1.0 CB 2:CYS192 3.4 34.1 1.0 CB 2:CYS195 3.4 32.4 1.0 N 2:CYS192 3.6 34.1 1.0 CA 2:CYS195 3.8 32.7 1.0 CA 2:CYS192 3.9 33.8 1.0 N 2:CYS292 4.3 40.0 1.0 O 2:CYS192 4.3 32.3 1.0 C 2:CYS192 4.3 33.7 1.0 CA 2:CYS292 4.3 40.5 1.0 C 2:PHE194 4.4 32.3 1.0 CA 2:CYS290 4.6 38.4 1.0 C 2:PRO191 4.7 34.3 1.0 N 2:PHE194 4.8 32.9 1.0 CB 2:PHE194 4.8 36.1 1.0 CA 2:PHE194 4.9 33.7 1.0 ND1 2:HIS286 4.9 38.5 1.0 O 2:HOH508 4.9 38.6 1.0 C 2:CYS290 4.9 37.6 1.0 CA 2:PRO191 5.0 34.8 1.0

D.Ghilarov, C.E.M.Stevenson, D.Y.Travin, J.Piskunova, M.Serebryakova, A.Maxwell, D.M.Lawson, K.Severinov. Architecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting A Ribosomally Synthesized Peptide Into A Dna Gyrase Poison. Mol. Cell V. 73 749 2019.
ISSN: ISSN 1097-4164
PubMed: 30661981
DOI: 10.1016/J.MOLCEL.2018.11.032