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Zinc in PDB 1xb8: Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin

Protein crystallography data

The structure of Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin, PDB code: 1xb8 was solved by A.Tigerstrom, F.Schwarz, G.Karlsson, M.Okvist, C.Alvarez-Rua, D.Maeder, F.T.Robb, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.25 / 2.00
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 113.140, 50.038, 57.722, 90.00, 119.51, 90.00
R / Rfree (%) 19.2 / 23.6

Zinc Binding Sites:

The binding sites of Zinc atom in the Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin (pdb code 1xb8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin, PDB code: 1xb8:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 1xb8

Go back to Zinc Binding Sites List in 1xb8
Zinc binding site 1 out of 2 in the Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1001

b:12.4
occ:1.00
ND1 A:HIS117 2.1 8.9 1.0
ND1 A:HIS46 2.1 6.5 1.0
O A:GLY45 2.2 8.6 1.0
SG A:CYS112 2.3 10.9 1.0
CE1 A:HIS117 3.0 12.7 1.0
CE1 A:HIS46 3.0 9.7 1.0
CG A:HIS117 3.1 10.9 1.0
CG A:HIS46 3.2 8.2 1.0
C A:GLY45 3.2 9.0 1.0
CA A:HIS46 3.2 8.7 1.0
CB A:CYS112 3.4 9.9 1.0
CB A:HIS117 3.5 13.1 1.0
SD A:MET121 3.5 10.9 1.0
CB A:HIS46 3.6 8.9 1.0
CB A:PHE114 3.6 11.7 1.0
N A:HIS46 3.7 8.6 1.0
NE2 A:HIS117 4.1 12.7 1.0
CE A:MET121 4.2 10.2 1.0
NE2 A:HIS46 4.2 8.8 1.0
CD2 A:HIS117 4.2 10.6 1.0
CD2 A:HIS46 4.3 12.1 1.0
CG A:PHE114 4.4 10.5 1.0
C A:HIS46 4.5 8.4 1.0
CA A:GLY45 4.5 9.9 1.0
N A:ASN47 4.7 7.9 1.0
N A:GLY45 4.7 9.9 1.0
CA A:PHE114 4.8 11.7 1.0
CD2 A:PHE114 4.8 12.3 1.0
O A:MET44 4.8 10.9 1.0
CA A:CYS112 4.8 10.1 1.0
N A:PHE114 4.8 11.4 1.0
C A:MET44 4.8 10.7 1.0
CA A:HIS117 5.0 11.8 1.0

Zinc binding site 2 out of 2 in 1xb8

Go back to Zinc Binding Sites List in 1xb8
Zinc binding site 2 out of 2 in the Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn5001

b:16.6
occ:1.00
ND1 C:HIS117 2.1 13.7 1.0
ND1 C:HIS46 2.2 13.7 1.0
O C:GLY45 2.2 12.4 1.0
SG C:CYS112 2.3 17.3 1.0
CE1 C:HIS117 3.0 14.6 1.0
CE1 C:HIS46 3.1 19.2 1.0
CG C:HIS117 3.2 13.7 1.0
CG C:HIS46 3.2 17.4 1.0
C C:GLY45 3.3 14.2 1.0
CA C:HIS46 3.3 15.8 1.0
CB C:CYS112 3.4 17.3 1.0
SD C:MET121 3.4 16.9 1.0
CB C:HIS117 3.5 16.9 1.0
CB C:HIS46 3.6 15.8 1.0
CB C:PHE114 3.6 12.8 1.0
N C:HIS46 3.7 15.1 1.0
CE C:MET121 4.1 20.8 1.0
NE2 C:HIS117 4.2 12.5 1.0
NE2 C:HIS46 4.3 17.5 1.0
CD2 C:HIS117 4.3 12.0 1.0
CD2 C:HIS46 4.3 16.1 1.0
CG C:PHE114 4.3 12.7 1.0
C C:HIS46 4.5 16.1 1.0
CA C:GLY45 4.6 14.7 1.0
N C:ASN47 4.7 16.4 1.0
CA C:CYS112 4.7 16.3 1.0
CD2 C:PHE114 4.8 13.2 1.0
N C:GLY45 4.8 14.4 1.0
N C:PHE114 4.8 12.5 1.0
CA C:PHE114 4.8 13.0 1.0
CG C:MET121 4.8 17.6 1.0
C C:MET44 5.0 15.2 1.0
O C:MET44 5.0 13.9 1.0

Reference:

A.Tigerstrom, F.Schwarz, G.Karlsson, M.Okvist, C.Alvarez-Rua, D.Maeder, F.T.Robb, L.Sjolin. Effects of A Novel Disulfide Bond and Engineered Electrostatic Interactions on the Thermostability of Azurin Biochemistry V. 43 12563 2004.
ISSN: ISSN 0006-2960
PubMed: 15449946
DOI: 10.1021/BI048926X
Page generated: Wed Dec 16 03:12:07 2020

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