Atomistry » Zinc » PDB 1x81-1xm4 » 1xb8
Atomistry »
  Zinc »
    PDB 1x81-1xm4 »
      1xb8 »

Zinc in PDB 1xb8: Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin

Protein crystallography data

The structure of Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin, PDB code: 1xb8 was solved by A.Tigerstrom, F.Schwarz, G.Karlsson, M.Okvist, C.Alvarez-Rua, D.Maeder, F.T.Robb, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 50.25 / 2.00
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 113.140, 50.038, 57.722, 90.00, 119.51, 90.00
R / Rfree (%) 19.2 / 23.6

Zinc Binding Sites:

The binding sites of Zinc atom in the Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin (pdb code 1xb8). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin, PDB code: 1xb8:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in 1xb8

Go back to Zinc Binding Sites List in 1xb8
Zinc binding site 1 out of 2 in the Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1001

b:12.4
occ:1.00
ND1 A:HIS117 2.1 8.9 1.0
ND1 A:HIS46 2.1 6.5 1.0
O A:GLY45 2.2 8.6 1.0
SG A:CYS112 2.3 10.9 1.0
CE1 A:HIS117 3.0 12.7 1.0
CE1 A:HIS46 3.0 9.7 1.0
CG A:HIS117 3.1 10.9 1.0
CG A:HIS46 3.2 8.2 1.0
C A:GLY45 3.2 9.0 1.0
CA A:HIS46 3.2 8.7 1.0
CB A:CYS112 3.4 9.9 1.0
CB A:HIS117 3.5 13.1 1.0
SD A:MET121 3.5 10.9 1.0
CB A:HIS46 3.6 8.9 1.0
CB A:PHE114 3.6 11.7 1.0
N A:HIS46 3.7 8.6 1.0
NE2 A:HIS117 4.1 12.7 1.0
CE A:MET121 4.2 10.2 1.0
NE2 A:HIS46 4.2 8.8 1.0
CD2 A:HIS117 4.2 10.6 1.0
CD2 A:HIS46 4.3 12.1 1.0
CG A:PHE114 4.4 10.5 1.0
C A:HIS46 4.5 8.4 1.0
CA A:GLY45 4.5 9.9 1.0
N A:ASN47 4.7 7.9 1.0
N A:GLY45 4.7 9.9 1.0
CA A:PHE114 4.8 11.7 1.0
CD2 A:PHE114 4.8 12.3 1.0
O A:MET44 4.8 10.9 1.0
CA A:CYS112 4.8 10.1 1.0
N A:PHE114 4.8 11.4 1.0
C A:MET44 4.8 10.7 1.0
CA A:HIS117 5.0 11.8 1.0

Zinc binding site 2 out of 2 in 1xb8

Go back to Zinc Binding Sites List in 1xb8
Zinc binding site 2 out of 2 in the Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Zn Substituted Form of D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn5001

b:16.6
occ:1.00
ND1 C:HIS117 2.1 13.7 1.0
ND1 C:HIS46 2.2 13.7 1.0
O C:GLY45 2.2 12.4 1.0
SG C:CYS112 2.3 17.3 1.0
CE1 C:HIS117 3.0 14.6 1.0
CE1 C:HIS46 3.1 19.2 1.0
CG C:HIS117 3.2 13.7 1.0
CG C:HIS46 3.2 17.4 1.0
C C:GLY45 3.3 14.2 1.0
CA C:HIS46 3.3 15.8 1.0
CB C:CYS112 3.4 17.3 1.0
SD C:MET121 3.4 16.9 1.0
CB C:HIS117 3.5 16.9 1.0
CB C:HIS46 3.6 15.8 1.0
CB C:PHE114 3.6 12.8 1.0
N C:HIS46 3.7 15.1 1.0
CE C:MET121 4.1 20.8 1.0
NE2 C:HIS117 4.2 12.5 1.0
NE2 C:HIS46 4.3 17.5 1.0
CD2 C:HIS117 4.3 12.0 1.0
CD2 C:HIS46 4.3 16.1 1.0
CG C:PHE114 4.3 12.7 1.0
C C:HIS46 4.5 16.1 1.0
CA C:GLY45 4.6 14.7 1.0
N C:ASN47 4.7 16.4 1.0
CA C:CYS112 4.7 16.3 1.0
CD2 C:PHE114 4.8 13.2 1.0
N C:GLY45 4.8 14.4 1.0
N C:PHE114 4.8 12.5 1.0
CA C:PHE114 4.8 13.0 1.0
CG C:MET121 4.8 17.6 1.0
C C:MET44 5.0 15.2 1.0
O C:MET44 5.0 13.9 1.0

Reference:

A.Tigerstrom, F.Schwarz, G.Karlsson, M.Okvist, C.Alvarez-Rua, D.Maeder, F.T.Robb, L.Sjolin. Effects of A Novel Disulfide Bond and Engineered Electrostatic Interactions on the Thermostability of Azurin Biochemistry V. 43 12563 2004.
ISSN: ISSN 0006-2960
PubMed: 15449946
DOI: 10.1021/BI048926X
Page generated: Wed Oct 16 20:22:07 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy