Zinc in PDB 9ich: Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar)

Protein crystallography data

The structure of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar), PDB code: 9ich was solved by H.Pelletier, M.R.Sawaya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.90
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 179.221, 57.678, 48.500, 90.00, 90.00, 90.00
R / Rfree (%) 17.6 / n/a

Other elements in 9ich:

The structure of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar) also contains other interesting chemical elements:

Sodium (Na) 2 atoms

Zinc Binding Sites:

The binding sites of Zinc atom in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar) (pdb code 9ich). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 3 binding sites of Zinc where determined in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar), PDB code: 9ich:
Jump to Zinc binding site number: 1; 2; 3;

Zinc binding site 1 out of 3 in 9ich

Go back to Zinc Binding Sites List in 9ich
Zinc binding site 1 out of 3 in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn339

b:30.0
occ:0.37
O1A A:DGT338 1.4 83.1 0.5
OD1 A:ASP190 2.6 45.6 1.0
O1G A:DGT338 2.6 33.0 0.5
O3G A:DGT338 2.7 39.6 0.5
PA A:DGT338 2.8 84.6 0.5
O2B A:DGT338 2.8 76.5 0.5
OD2 A:ASP192 2.9 29.7 1.0
PG A:DGT338 3.1 41.8 0.5
O3A A:DGT338 3.3 79.9 0.5
O5' A:DGT338 3.4 84.0 0.5
PB A:DGT338 3.4 74.9 0.5
O A:ASP190 3.5 35.6 1.0
O3B A:DGT338 3.5 60.3 0.5
CG A:ASP190 3.7 49.9 1.0
O A:HOH522 3.9 50.6 1.0
O2A A:DGT338 4.0 82.1 0.5
O A:HOH656 4.1 76.2 1.0
CG A:ASP192 4.2 34.1 1.0
N A:ASP190 4.2 29.3 1.0
ZN A:ZN340 4.3 30.0 0.4
C A:ASP190 4.3 28.3 1.0
O A:HOH502 4.4 9.8 1.0
OD2 A:ASP190 4.4 58.5 1.0
O2G A:DGT338 4.5 49.5 0.5
CA A:ASP190 4.7 33.3 1.0
CB A:ASP190 4.8 42.9 1.0
O1B A:DGT338 4.9 76.9 0.5
CB A:ASP192 4.9 25.4 1.0

Zinc binding site 2 out of 3 in 9ich

Go back to Zinc Binding Sites List in 9ich
Zinc binding site 2 out of 3 in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn340

b:30.0
occ:0.37
O3' P:DG7 3.1 0.0 1.0
OD2 A:ASP190 3.1 58.5 1.0
OD2 A:ASP256 3.2 41.3 1.0
OD1 A:ASP192 3.3 44.1 1.0
OD2 A:ASP192 3.3 29.7 1.0
CG A:ASP190 3.5 49.9 1.0
CG A:ASP192 3.6 34.1 1.0
CG A:ASP256 3.7 30.9 1.0
OD1 A:ASP190 3.7 45.6 1.0
O1A A:DGT338 3.8 83.1 0.5
OD1 A:ASP256 4.1 30.0 1.0
CB A:ASP256 4.1 20.9 1.0
C4' P:DG7 4.2 0.0 1.0
C3' P:DG7 4.2 0.0 1.0
ZN A:ZN339 4.3 30.0 0.4
PA A:DGT338 4.4 84.6 0.5
CB A:ASP190 4.5 42.9 1.0
O2A A:DGT338 4.5 82.1 0.5
O5' P:DG7 4.5 0.0 1.0
O5' A:DGT338 4.6 84.0 0.5
O A:HOH657 4.6 59.3 1.0
C5' P:DG7 4.7 0.0 1.0
NH2 A:ARG254 4.8 24.9 1.0
CB A:ASP192 4.8 25.4 1.0

Zinc binding site 3 out of 3 in 9ich

Go back to Zinc Binding Sites List in 9ich
Zinc binding site 3 out of 3 in the Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar)


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Dna Polymerase Beta (Pol B) (E.C.2.7.7.7) Complexed with Seven Base Pairs of Dna; Soaked in the Presence of Dgtp (1 Millimolar) and ZNCL2 (1 Millimolar) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn343

b:30.0
occ:0.74
ND1 A:HIS51 2.4 36.5 1.0
CG A:HIS51 3.1 38.4 1.0
CE1 A:HIS51 3.2 37.1 1.0
CB A:HIS51 3.5 42.6 1.0
CD2 A:HIS51 4.1 37.4 1.0
NE2 A:HIS51 4.1 36.1 1.0
CA A:HIS51 4.2 42.1 1.0
N A:LYS52 4.8 39.2 1.0

Reference:

H.Pelletier, M.R.Sawaya. Characterization of the Metal Ion Binding Helix-Hairpin-Helix Motifs in Human Dna Polymerase Beta By X-Ray Structural Analysis. Biochemistry V. 35 12778 1996.
ISSN: ISSN 0006-2960
PubMed: 8841120
DOI: 10.1021/BI960790I
Page generated: Wed Dec 16 14:05:23 2020

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