Zinc in PDB 9gku: Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57

The structure of Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57, PDB code: 9gku was solved by L.G.Graf, L.Lammers, G.J.Palm, S.Schulze, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.56 / 1.48
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	103.861, 103.698, 104.499, 113.6, 93, 120.61
R / Rfree (%)	17.3 / 19.9

The structure of Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57 also contains other interesting chemical elements:

Potassium

(K)

8 atoms

The binding sites of Zinc atom in the Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57 (pdb code 9gku). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57, PDB code: 9gku:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Zinc binding site 1 out of 8 in the Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57 within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn403 b:20.2 occ:0.84 OD2 A:ASP278 2.0 19.5 1.0 OD1 A:ASP190 2.0 23.1 1.0 ND1 A:HIS192 2.2 21.4 1.0 O A:ACT401 2.3 23.9 1.0 OXT A:ACT401 2.4 24.6 1.0 C A:ACT401 2.6 23.2 1.0 CG A:ASP190 2.7 22.6 1.0 OD2 A:ASP190 2.7 20.6 1.0 CG A:ASP278 3.0 20.7 1.0 CE1 A:HIS192 3.1 20.7 1.0 CG A:HIS192 3.3 20.0 1.0 OD1 A:ASP278 3.3 20.3 1.0 CB A:HIS192 3.6 20.3 1.0 N A:HIS192 3.9 21.1 1.0 O6 A:BGC402 4.0 24.3 0.5 CH3 A:ACT401 4.1 24.2 1.0 CB A:ASP190 4.1 20.4 1.0 CG1 A:VAL191 4.1 20.1 1.0 NE2 A:HIS152 4.2 23.5 1.0 CA A:GLY320 4.2 22.4 1.0 N A:VAL191 4.2 19.6 1.0 NE2 A:HIS192 4.3 21.1 1.0 CB A:ASP278 4.3 19.0 1.0 CD2 A:HIS192 4.3 20.1 1.0 CA A:HIS192 4.4 19.4 1.0 CE2 A:TYR322 4.4 20.4 1.0 N A:GLY320 4.6 21.2 1.0 NE2 A:HIS153 4.6 21.6 1.0 CE1 A:HIS152 4.6 23.5 1.0 C A:ASP190 4.7 20.1 1.0 OH A:TYR322 4.8 23.2 1.0 C A:VAL191 4.8 20.9 1.0 CA A:ASP190 4.8 20.3 1.0 CA A:VAL191 4.9 18.6 1.0

Zinc binding site 2 out of 8 in the Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57 within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn403 b:22.1 occ:0.85 OD1 B:ASP190 1.9 20.8 1.0 OD2 B:ASP278 2.0 20.1 1.0 ND1 B:HIS192 2.2 21.5 1.0 O B:ACT401 2.3 30.8 1.0 OXT B:ACT401 2.5 26.0 1.0 CG B:ASP190 2.6 24.6 1.0 OD2 B:ASP190 2.6 25.1 1.0 C B:ACT401 2.6 28.1 1.0 CG B:ASP278 3.0 19.9 1.0 CE1 B:HIS192 3.1 23.4 1.0 CG B:HIS192 3.2 24.6 1.0 OD1 B:ASP278 3.3 22.2 1.0 CB B:HIS192 3.6 23.4 1.0 N B:HIS192 3.8 22.9 1.0 CB B:ASP190 4.1 21.0 1.0 O6 B:BGC402 4.1 26.6 0.5 CH3 B:ACT401 4.1 27.2 1.0 CG1 B:VAL191 4.2 19.7 1.0 NE2 B:HIS152 4.2 24.2 1.0 N B:VAL191 4.2 21.4 1.0 CA B:GLY320 4.2 23.0 1.0 NE2 B:HIS192 4.3 23.2 1.0 CB B:ASP278 4.3 20.4 1.0 CA B:HIS192 4.3 23.4 1.0 CD2 B:HIS192 4.3 26.2 1.0 CE2 B:TYR322 4.4 21.3 1.0 N B:GLY320 4.5 23.6 1.0 CE1 B:HIS152 4.5 27.1 1.0 NE2 B:HIS153 4.6 27.1 1.0 C B:ASP190 4.7 22.9 1.0 C B:VAL191 4.7 21.3 1.0 CA B:ASP190 4.8 20.7 1.0 OH B:TYR322 4.8 25.6 1.0 CA B:VAL191 4.9 22.1 1.0

Zinc binding site 3 out of 8 in the Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57 within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn404 b:21.1 occ:0.86 OD2 C:ASP278 2.0 20.1 1.0 OD1 C:ASP190 2.0 21.6 1.0 ND1 C:HIS192 2.2 21.8 1.0 OXT C:ACT401 2.2 22.0 0.8 O C:ACT401 2.4 21.8 0.8 C C:ACT401 2.6 20.1 0.8 OD2 C:ASP190 2.6 22.0 1.0 CG C:ASP190 2.7 21.7 1.0 CG C:ASP278 3.0 20.0 1.0 CE1 C:HIS192 3.0 21.4 1.0 CG C:HIS192 3.2 21.2 1.0 OD1 C:ASP278 3.3 20.9 1.0 CB C:HIS192 3.6 21.2 1.0 N C:HIS192 3.9 20.1 1.0 CH3 C:ACT401 4.1 21.7 0.8 CB C:ASP190 4.1 21.9 1.0 CG1 C:VAL191 4.1 19.5 1.0 NE2 C:HIS152 4.2 21.8 1.0 CA C:GLY320 4.2 23.4 1.0 N C:VAL191 4.2 19.2 1.0 NE2 C:HIS192 4.2 20.2 1.0 CB C:ASP278 4.3 18.1 1.0 CD2 C:HIS192 4.3 22.0 1.0 CA C:HIS192 4.4 19.8 1.0 O6 C:BGC402 4.4 27.4 0.5 O4 C:BGC402 4.4 46.2 0.5 CE2 C:TYR322 4.4 19.8 1.0 N C:GLY320 4.5 21.9 1.0 CE1 C:HIS152 4.5 22.9 1.0 NE2 C:HIS153 4.6 22.8 1.0 C C:ASP190 4.7 20.5 1.0 OH C:TYR322 4.8 23.3 1.0 C C:VAL191 4.8 21.1 1.0 CA C:ASP190 4.8 19.3 1.0 CA C:VAL191 4.9 19.6 1.0 C C:GLY320 5.0 22.7 1.0

Zinc binding site 4 out of 8 in the Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57 within 5.0Å range: probe atom residue distance (Å) B Occ D:Zn402 b:21.1 occ:0.87 OD1 D:ASP190 2.0 20.1 1.0 OD2 D:ASP278 2.0 20.6 1.0 O D:ACT401 2.2 25.2 1.0 ND1 D:HIS192 2.2 21.9 1.0 OXT D:ACT401 2.4 26.9 1.0 C D:ACT401 2.6 26.2 1.0 OD2 D:ASP190 2.6 21.6 1.0 CG D:ASP190 2.6 22.1 1.0 CG D:ASP278 3.0 20.5 1.0 CE1 D:HIS192 3.1 22.1 1.0 CG D:HIS192 3.2 21.3 1.0 OD1 D:ASP278 3.3 22.3 1.0 CB D:HIS192 3.6 20.2 1.0 N D:HIS192 3.9 19.7 1.0 O D:HOH695 4.1 41.4 1.0 CB D:ASP190 4.1 20.1 1.0 CH3 D:ACT401 4.1 25.3 1.0 NE2 D:HIS152 4.2 24.9 1.0 O D:HOH693 4.2 41.4 1.0 CG1 D:VAL191 4.2 18.5 1.0 CA D:GLY320 4.2 22.6 1.0 N D:VAL191 4.2 19.5 1.0 NE2 D:HIS192 4.3 23.6 1.0 CD2 D:HIS192 4.3 24.1 1.0 CB D:ASP278 4.3 18.0 1.0 CA D:HIS192 4.4 20.9 1.0 CE2 D:TYR322 4.4 21.4 1.0 N D:GLY320 4.5 21.2 1.0 CE1 D:HIS152 4.5 24.4 1.0 NE2 D:HIS153 4.6 25.5 1.0 C D:ASP190 4.7 21.7 1.0 C D:VAL191 4.8 21.1 1.0 CA D:ASP190 4.8 19.1 1.0 OH D:TYR322 4.8 25.8 1.0 CA D:VAL191 4.9 20.7 1.0 C D:GLY320 5.0 21.0 1.0

Zinc binding site 5 out of 8 in the Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57 within 5.0Å range: probe atom residue distance (Å) B Occ E:Zn404 b:18.3 occ:0.81 OD2 E:ASP278 2.0 18.5 1.0 OD1 E:ASP190 2.0 17.9 1.0 O E:ACT401 2.2 23.3 1.0 ND1 E:HIS192 2.2 20.8 1.0 OXT E:ACT401 2.5 21.6 1.0 C E:ACT401 2.6 20.8 1.0 OD2 E:ASP190 2.6 20.9 1.0 CG E:ASP190 2.6 19.9 1.0 CG E:ASP278 3.0 18.1 1.0 CE1 E:HIS192 3.1 18.8 1.0 CG E:HIS192 3.3 18.5 1.0 OD1 E:ASP278 3.3 19.5 1.0 CB E:HIS192 3.6 19.3 1.0 N E:HIS192 3.9 18.8 1.0 O6 E:BGC402 3.9 24.5 0.5 CB E:ASP190 4.1 18.6 1.0 CH3 E:ACT401 4.1 21.1 1.0 CG1 E:VAL191 4.2 18.2 1.0 NE2 E:HIS152 4.2 19.8 1.0 CA E:GLY320 4.2 20.7 1.0 N E:VAL191 4.2 17.9 1.0 NE2 E:HIS192 4.3 19.0 1.0 CB E:ASP278 4.3 18.2 1.0 CD2 E:HIS192 4.3 22.0 1.0 CA E:HIS192 4.4 17.9 1.0 CE2 E:TYR322 4.5 21.9 1.0 N E:GLY320 4.5 20.5 1.0 CE1 E:HIS152 4.6 19.7 1.0 NE2 E:HIS153 4.6 21.3 1.0 C E:ASP190 4.7 16.2 1.0 C E:VAL191 4.8 19.9 1.0 CA E:ASP190 4.8 18.0 1.0 OH E:TYR322 4.8 22.9 1.0 CA E:VAL191 4.9 17.4 1.0 C6 E:BGC402 5.0 27.4 0.5 C E:GLY320 5.0 22.2 1.0

Zinc binding site 6 out of 8 in the Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57 within 5.0Å range: probe atom residue distance (Å) B Occ F:Zn403 b:19.4 occ:0.87 OD2 F:ASP278 2.0 17.9 1.0 OD1 F:ASP190 2.0 18.4 1.0 ND1 F:HIS192 2.2 20.6 1.0 O F:ACT401 2.2 23.3 1.0 OXT F:ACT401 2.4 21.6 1.0 OD2 F:ASP190 2.6 20.7 1.0 C F:ACT401 2.6 20.1 1.0 CG F:ASP190 2.6 18.9 1.0 CG F:ASP278 2.9 18.7 1.0 CE1 F:HIS192 3.1 20.3 1.0 OD1 F:ASP278 3.3 19.5 1.0 CG F:HIS192 3.3 19.1 1.0 O F:HOH589 3.6 37.4 1.0 CB F:HIS192 3.7 18.8 1.0 N F:HIS192 3.9 17.0 1.0 CB F:ASP190 4.1 18.2 1.0 CH3 F:ACT401 4.1 21.6 1.0 CG1 F:VAL191 4.2 18.5 1.0 NE2 F:HIS152 4.2 20.3 1.0 CA F:GLY320 4.2 20.9 1.0 N F:VAL191 4.2 18.7 1.0 NE2 F:HIS192 4.2 19.1 1.0 CB F:ASP278 4.3 17.4 1.0 CD2 F:HIS192 4.4 19.9 1.0 CA F:HIS192 4.4 17.9 1.0 CE2 F:TYR322 4.5 20.6 1.0 N F:GLY320 4.5 19.2 1.0 CE1 F:HIS152 4.6 20.3 1.0 NE2 F:HIS153 4.7 21.3 1.0 C F:ASP190 4.7 17.9 1.0 C F:VAL191 4.8 18.8 1.0 CA F:ASP190 4.8 18.6 1.0 OH F:TYR322 4.8 24.0 1.0 CA F:VAL191 4.9 18.2 1.0 C F:GLY320 5.0 21.1 1.0

Zinc binding site 7 out of 8 in the Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57 within 5.0Å range: probe atom residue distance (Å) B Occ G:Zn403 b:20.3 occ:0.86 OD1 G:ASP190 2.0 19.8 1.0 OD2 G:ASP278 2.0 21.1 1.0 ND1 G:HIS192 2.2 24.0 1.0 OXT G:ACT401 2.3 28.7 1.0 O G:ACT401 2.4 25.8 1.0 C G:ACT401 2.6 29.2 1.0 CG G:ASP190 2.6 22.3 1.0 OD2 G:ASP190 2.6 21.2 1.0 CG G:ASP278 3.0 19.2 1.0 CE1 G:HIS192 3.1 23.4 1.0 CG G:HIS192 3.3 24.0 1.0 OD1 G:ASP278 3.3 19.0 1.0 CB G:HIS192 3.6 24.7 1.0 N G:HIS192 3.9 21.7 1.0 CH3 G:ACT401 4.1 27.3 1.0 CB G:ASP190 4.1 20.4 1.0 CA G:GLY320 4.1 19.8 1.0 NE2 G:HIS152 4.2 22.2 1.0 CG1 G:VAL191 4.2 20.9 1.0 NE2 G:HIS192 4.3 25.5 1.0 N G:VAL191 4.3 20.4 1.0 CB G:ASP278 4.3 19.9 1.0 CD2 G:HIS192 4.4 25.5 1.0 CE2 G:TYR322 4.4 20.2 1.0 CA G:HIS192 4.4 22.7 1.0 N G:GLY320 4.5 21.4 1.0 CE1 G:HIS152 4.5 24.3 1.0 O G:HOH714 4.6 43.7 1.0 NE2 G:HIS153 4.6 26.1 1.0 OH G:TYR322 4.7 26.8 1.0 C G:ASP190 4.7 22.8 1.0 CA G:ASP190 4.8 20.4 1.0 C G:VAL191 4.8 21.9 1.0 C G:GLY320 5.0 23.1 1.0 CA G:VAL191 5.0 21.4 1.0

Zinc binding site 8 out of 8 in the Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Crystal Structure of Propanil Hydrolase (Prph) From Sphingomonas Sp. Y57 within 5.0Å range: probe atom residue distance (Å) B Occ H:Zn403 b:22.2 occ:0.88 OD1 H:ASP190 2.0 21.1 1.0 OD2 H:ASP278 2.0 21.6 1.0 OXT H:ACT401 2.2 27.7 0.9 ND1 H:HIS192 2.2 26.2 1.0 O H:ACT401 2.3 26.4 0.9 C H:ACT401 2.5 28.8 0.9 CG H:ASP190 2.7 24.7 1.0 OD2 H:ASP190 2.7 23.1 1.0 CG H:ASP278 3.0 18.3 1.0 CE1 H:HIS192 3.1 25.6 1.0 CG H:HIS192 3.3 26.3 1.0 OD1 H:ASP278 3.3 21.0 1.0 CB H:HIS192 3.7 27.4 1.0 N H:HIS192 3.9 21.8 1.0 CH3 H:ACT401 4.0 26.5 0.9 CB H:ASP190 4.1 23.8 1.0 CA H:GLY320 4.2 22.3 1.0 CG1 H:VAL191 4.2 21.8 1.0 NE2 H:HIS152 4.2 24.9 1.0 NE2 H:HIS192 4.2 28.1 1.0 N H:VAL191 4.3 22.5 1.0 CB H:ASP278 4.3 20.2 1.0 CD2 H:HIS192 4.4 29.3 1.0 CE2 H:TYR322 4.4 21.7 1.0 CA H:HIS192 4.4 24.4 1.0 N H:GLY320 4.5 22.9 1.0 CE1 H:HIS152 4.6 26.9 1.0 NE2 H:HIS153 4.6 27.3 1.0 OH H:TYR322 4.7 27.3 1.0 C H:ASP190 4.8 23.9 1.0 O H:HOH698 4.8 43.1 1.0 C H:VAL191 4.8 22.8 1.0 CA H:ASP190 4.8 23.1 1.0 CA H:VAL191 5.0 23.7 1.0 C H:GLY320 5.0 25.1 1.0

L.G.Graf, C.Moreno-Yruela, C.Qin, S.Schulze, G.J.Palm, O.Schmoeker, N.Wang, D.Hocking, L.Jebeli, B.Girbardt, L.Berndt, D.M.Weis, M.Janetzky, D.Zuehlke, S.Sievers, R.A.Strugnell, C.A.Olsen, K.Hofmann, M.Lammers. Distribution and Diversity of Classical Deacylases in Bacteria Nature Communications 2024.