Zinc in PDB 9fyl: Lacto-N-Biosidase From Treponema Denticola Atcc 35405, Histag Bound in Active Site
Protein crystallography data
The structure of Lacto-N-Biosidase From Treponema Denticola Atcc 35405, Histag Bound in Active Site, PDB code: 9fyl
was solved by
M.Vuillemin,
S.Siebenhaar,
B.Zeuner,
J.P.Morth,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
46.31 /
2.24
|
Space group
|
C 1 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
128.917,
89.471,
93.757,
90,
127,
90
|
R / Rfree (%)
|
23.2 /
26.9
|
Zinc Binding Sites:
The binding sites of Zinc atom in the Lacto-N-Biosidase From Treponema Denticola Atcc 35405, Histag Bound in Active Site
(pdb code 9fyl). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the
Lacto-N-Biosidase From Treponema Denticola Atcc 35405, Histag Bound in Active Site, PDB code: 9fyl:
Jump to Zinc binding site number:
1;
2;
Zinc binding site 1 out
of 2 in 9fyl
Go back to
Zinc Binding Sites List in 9fyl
Zinc binding site 1 out
of 2 in the Lacto-N-Biosidase From Treponema Denticola Atcc 35405, Histag Bound in Active Site
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Lacto-N-Biosidase From Treponema Denticola Atcc 35405, Histag Bound in Active Site within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Zn401
b:75.6
occ:1.00
|
OE1
|
A:GLU140
|
2.0
|
67.9
|
1.0
|
SG
|
A:CYS198
|
2.2
|
86.2
|
1.0
|
SG
|
A:CYS201
|
2.2
|
295.0
|
1.0
|
SG
|
A:CYS157
|
2.4
|
83.7
|
1.0
|
HB2
|
A:CYS201
|
2.9
|
124.0
|
1.0
|
CD
|
A:GLU140
|
2.9
|
77.3
|
1.0
|
HA
|
A:CYS157
|
3.1
|
91.0
|
1.0
|
CB
|
A:CYS201
|
3.1
|
103.0
|
1.0
|
OE2
|
A:GLU140
|
3.2
|
69.9
|
1.0
|
HB2
|
A:CYS157
|
3.3
|
100.3
|
1.0
|
CB
|
A:CYS157
|
3.3
|
83.3
|
1.0
|
HB3
|
A:LEU200
|
3.3
|
114.6
|
1.0
|
CB
|
A:CYS198
|
3.4
|
76.4
|
1.0
|
HB2
|
A:CYS198
|
3.4
|
92.1
|
1.0
|
H
|
A:CYS201
|
3.5
|
95.6
|
1.0
|
HB3
|
A:CYS198
|
3.5
|
92.1
|
1.0
|
HB2
|
A:LEU200
|
3.6
|
114.6
|
1.0
|
CA
|
A:CYS157
|
3.6
|
75.5
|
1.0
|
HA
|
A:GLU140
|
3.7
|
87.7
|
1.0
|
H
|
A:SER159
|
3.8
|
97.7
|
1.0
|
N
|
A:CYS201
|
3.8
|
79.3
|
1.0
|
H
|
A:SER158
|
3.8
|
96.4
|
1.0
|
HB2
|
A:SER159
|
3.8
|
88.9
|
1.0
|
HB3
|
A:CYS201
|
3.9
|
124.0
|
1.0
|
CB
|
A:LEU200
|
3.9
|
95.1
|
1.0
|
CA
|
A:CYS201
|
4.1
|
90.3
|
1.0
|
HB3
|
A:CYS157
|
4.2
|
100.3
|
1.0
|
N
|
A:SER158
|
4.2
|
80.0
|
1.0
|
C
|
A:CYS157
|
4.3
|
70.4
|
1.0
|
CG
|
A:GLU140
|
4.3
|
80.0
|
1.0
|
HB2
|
A:GLU140
|
4.4
|
90.7
|
1.0
|
C
|
A:LEU200
|
4.5
|
83.0
|
1.0
|
H
|
A:LEU200
|
4.5
|
95.7
|
1.0
|
HG
|
A:LEU200
|
4.6
|
111.2
|
1.0
|
CA
|
A:GLU140
|
4.6
|
72.7
|
1.0
|
HA
|
A:CYS201
|
4.6
|
108.8
|
1.0
|
N
|
A:SER159
|
4.6
|
81.1
|
1.0
|
CB
|
A:GLU140
|
4.6
|
75.2
|
1.0
|
HG
|
A:SER159
|
4.7
|
85.4
|
1.0
|
HG3
|
A:GLU140
|
4.7
|
96.4
|
1.0
|
CB
|
A:SER159
|
4.7
|
73.7
|
1.0
|
H
|
A:GLY194
|
4.7
|
96.4
|
1.0
|
CA
|
A:CYS198
|
4.8
|
81.9
|
1.0
|
CG
|
A:LEU200
|
4.8
|
92.3
|
1.0
|
CA
|
A:LEU200
|
4.8
|
84.0
|
1.0
|
O
|
A:GLU140
|
4.8
|
74.4
|
1.0
|
HG2
|
A:GLU140
|
4.9
|
96.4
|
1.0
|
HA
|
A:CYS198
|
4.9
|
98.7
|
1.0
|
N
|
A:CYS157
|
4.9
|
69.8
|
1.0
|
|
Zinc binding site 2 out
of 2 in 9fyl
Go back to
Zinc Binding Sites List in 9fyl
Zinc binding site 2 out
of 2 in the Lacto-N-Biosidase From Treponema Denticola Atcc 35405, Histag Bound in Active Site
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Lacto-N-Biosidase From Treponema Denticola Atcc 35405, Histag Bound in Active Site within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Zn401
b:183.3
occ:1.00
|
SG
|
B:CYS157
|
1.8
|
179.4
|
1.0
|
OE1
|
B:GLU140
|
1.9
|
81.2
|
1.0
|
SG
|
B:CYS198
|
2.1
|
95.4
|
1.0
|
SG
|
B:CYS201
|
2.1
|
81.5
|
1.0
|
CD
|
B:GLU140
|
2.8
|
77.6
|
1.0
|
HB2
|
B:CYS201
|
2.9
|
85.6
|
1.0
|
OE2
|
B:GLU140
|
3.0
|
78.6
|
1.0
|
CB
|
B:CYS201
|
3.1
|
71.0
|
1.0
|
CB
|
B:CYS198
|
3.1
|
76.8
|
1.0
|
HB2
|
B:CYS198
|
3.2
|
92.6
|
1.0
|
CB
|
B:CYS157
|
3.2
|
67.8
|
1.0
|
HB3
|
B:CYS198
|
3.3
|
92.6
|
1.0
|
H
|
B:CYS201
|
3.3
|
88.7
|
1.0
|
HA
|
B:CYS157
|
3.3
|
87.1
|
1.0
|
HB2
|
B:CYS157
|
3.4
|
81.8
|
1.0
|
HB2
|
B:LEU200
|
3.5
|
109.3
|
1.0
|
O
|
B:HOH512
|
3.7
|
78.8
|
1.0
|
HA
|
B:GLU140
|
3.7
|
93.6
|
1.0
|
N
|
B:CYS201
|
3.7
|
73.5
|
1.0
|
CA
|
B:CYS157
|
3.8
|
72.2
|
1.0
|
HB3
|
B:CYS201
|
3.8
|
85.6
|
1.0
|
H
|
B:SER158
|
4.0
|
83.7
|
1.0
|
H
|
B:SER159
|
4.0
|
91.5
|
1.0
|
HB3
|
B:CYS157
|
4.0
|
81.8
|
1.0
|
CA
|
B:CYS201
|
4.0
|
77.1
|
1.0
|
CG
|
B:GLU140
|
4.1
|
76.8
|
1.0
|
HB2
|
B:SER159
|
4.2
|
105.0
|
1.0
|
H
|
B:LEU200
|
4.2
|
106.7
|
1.0
|
HB2
|
B:GLU140
|
4.3
|
94.3
|
1.0
|
N
|
B:SER158
|
4.4
|
69.4
|
1.0
|
C
|
B:CYS157
|
4.4
|
65.4
|
1.0
|
CB
|
B:LEU200
|
4.4
|
90.7
|
1.0
|
CA
|
B:GLU140
|
4.5
|
77.6
|
1.0
|
CA
|
B:CYS198
|
4.5
|
82.1
|
1.0
|
HG3
|
B:GLU140
|
4.6
|
92.6
|
1.0
|
CB
|
B:GLU140
|
4.6
|
78.3
|
1.0
|
H
|
B:GLY194
|
4.6
|
90.1
|
1.0
|
O
|
B:GLU140
|
4.6
|
74.6
|
1.0
|
HB3
|
B:LEU200
|
4.6
|
109.3
|
1.0
|
C
|
B:LEU200
|
4.6
|
84.7
|
1.0
|
HA
|
B:CYS201
|
4.6
|
93.0
|
1.0
|
HA
|
B:CYS198
|
4.7
|
98.9
|
1.0
|
HG2
|
B:GLU140
|
4.7
|
92.6
|
1.0
|
HD11
|
B:LEU200
|
4.8
|
122.1
|
1.0
|
N
|
B:SER159
|
4.8
|
75.9
|
1.0
|
N
|
B:LEU200
|
4.9
|
88.6
|
1.0
|
CA
|
B:LEU200
|
4.9
|
89.3
|
1.0
|
HD2
|
B:PRO199
|
4.9
|
104.1
|
1.0
|
H
|
B:GLU195
|
4.9
|
78.1
|
1.0
|
HG
|
B:SER159
|
4.9
|
102.9
|
1.0
|
CB
|
B:SER159
|
5.0
|
87.2
|
1.0
|
OG
|
B:SER159
|
5.0
|
85.4
|
1.0
|
|
Reference:
M.Vuillemin,
J.Muschiol,
Y.Zhang,
J.Holck,
K.Barrett,
J.P.Morth,
A.S.Meyer,
B.Zeuner.
Discovery of Lacto-N-Biosidases and A Novel N-Acetyllactosaminidase Activity in the Cazy Family GH20: Functional Diversity and Structural Insights. Chembiochem 00710 2024.
ISSN: ESSN 1439-7633
PubMed: 39239753
DOI: 10.1002/CBIC.202400710
Page generated: Thu Oct 31 15:33:30 2024
|