Zinc in PDB 9b12: Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1
Enzymatic activity of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1
All present enzymatic activity of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1:
2.3.2.31;
Protein crystallography data
The structure of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1, PDB code: 9b12
was solved by
M.A.Michel,
S.Scutts,
D.Komander,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
46.15 /
1.81
|
Space group
|
C 1 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
110.413,
70.191,
193.944,
90,
102.25,
90
|
R / Rfree (%)
|
23.1 /
26.9
|
Other elements in 9b12:
The structure of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1 also contains other interesting chemical elements:
Zinc Binding Sites:
The binding sites of Zinc atom in the Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1
(pdb code 9b12). This binding sites where shown within
5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the
Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1, PDB code: 9b12:
Jump to Zinc binding site number:
1;
2;
3;
4;
Zinc binding site 1 out
of 4 in 9b12
Go back to
Zinc Binding Sites List in 9b12
Zinc binding site 1 out
of 4 in the Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 1 of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
G:Zn401
b:35.2
occ:1.00
|
SG
|
G:CYS359
|
2.3
|
42.7
|
1.0
|
SG
|
G:CYS356
|
2.3
|
38.7
|
1.0
|
SG
|
G:CYS370
|
2.3
|
27.7
|
1.0
|
SG
|
G:CYS373
|
2.3
|
34.9
|
1.0
|
CB
|
G:CYS370
|
3.1
|
29.7
|
1.0
|
CB
|
G:CYS356
|
3.2
|
31.8
|
1.0
|
CB
|
G:CYS359
|
3.4
|
30.6
|
1.0
|
CB
|
G:CYS373
|
3.5
|
35.0
|
1.0
|
N
|
G:CYS359
|
3.8
|
37.7
|
1.0
|
N
|
G:CYS373
|
3.8
|
36.3
|
1.0
|
CA
|
G:CYS359
|
4.1
|
34.7
|
1.0
|
CA
|
G:CYS373
|
4.2
|
34.6
|
1.0
|
CA
|
G:CYS370
|
4.6
|
30.2
|
1.0
|
CA
|
G:CYS356
|
4.6
|
27.4
|
1.0
|
C
|
G:CYS359
|
4.6
|
36.3
|
1.0
|
O
|
G:HOH505
|
4.7
|
32.8
|
1.0
|
CB
|
G:PHE361
|
4.8
|
40.6
|
1.0
|
N
|
G:THR360
|
4.8
|
39.9
|
1.0
|
C
|
G:SER358
|
4.8
|
36.7
|
1.0
|
C
|
G:CYS373
|
4.8
|
34.7
|
1.0
|
N
|
G:PHE361
|
4.9
|
35.5
|
1.0
|
CB
|
G:ILE372
|
4.9
|
32.5
|
1.0
|
C
|
G:ILE372
|
4.9
|
31.2
|
1.0
|
CB
|
G:SER358
|
5.0
|
38.6
|
1.0
|
N
|
G:GLU374
|
5.0
|
36.3
|
1.0
|
|
Zinc binding site 2 out
of 4 in 9b12
Go back to
Zinc Binding Sites List in 9b12
Zinc binding site 2 out
of 4 in the Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 2 of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
H:Zn401
b:30.6
occ:1.00
|
SG
|
H:CYS370
|
2.3
|
33.6
|
1.0
|
SG
|
H:CYS373
|
2.3
|
30.6
|
1.0
|
SG
|
H:CYS356
|
2.3
|
33.8
|
1.0
|
SG
|
H:CYS359
|
2.3
|
34.8
|
1.0
|
CB
|
H:CYS370
|
3.1
|
27.9
|
1.0
|
CB
|
H:CYS356
|
3.1
|
30.9
|
1.0
|
CB
|
H:CYS359
|
3.4
|
30.2
|
1.0
|
CB
|
H:CYS373
|
3.4
|
34.2
|
1.0
|
N
|
H:CYS373
|
3.8
|
41.5
|
1.0
|
N
|
H:CYS359
|
3.8
|
28.1
|
1.0
|
CA
|
H:CYS359
|
4.1
|
36.5
|
1.0
|
CA
|
H:CYS373
|
4.2
|
36.4
|
1.0
|
CA
|
H:CYS370
|
4.6
|
25.4
|
1.0
|
CA
|
H:CYS356
|
4.6
|
32.5
|
1.0
|
CB
|
H:PHE361
|
4.6
|
30.6
|
1.0
|
C
|
H:CYS359
|
4.7
|
39.2
|
1.0
|
N
|
H:PHE361
|
4.8
|
35.0
|
1.0
|
N
|
H:THR360
|
4.8
|
37.4
|
1.0
|
CB
|
H:ILE372
|
4.8
|
32.9
|
1.0
|
C
|
H:CYS373
|
4.8
|
26.9
|
1.0
|
CB
|
H:SER358
|
4.9
|
35.0
|
1.0
|
O
|
H:HOH504
|
4.9
|
35.1
|
1.0
|
C
|
H:SER358
|
4.9
|
27.4
|
1.0
|
C
|
H:ILE372
|
4.9
|
40.0
|
1.0
|
N
|
H:ILE372
|
5.0
|
29.9
|
1.0
|
|
Zinc binding site 3 out
of 4 in 9b12
Go back to
Zinc Binding Sites List in 9b12
Zinc binding site 3 out
of 4 in the Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 3 of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
I:Zn401
b:28.0
occ:1.00
|
SG
|
I:CYS359
|
2.3
|
34.0
|
1.0
|
SG
|
I:CYS373
|
2.3
|
26.4
|
1.0
|
SG
|
I:CYS356
|
2.3
|
30.4
|
1.0
|
SG
|
I:CYS370
|
2.3
|
21.8
|
1.0
|
CB
|
I:CYS370
|
3.1
|
28.7
|
1.0
|
CB
|
I:CYS356
|
3.2
|
17.1
|
1.0
|
CB
|
I:CYS359
|
3.4
|
29.4
|
1.0
|
CB
|
I:CYS373
|
3.4
|
26.6
|
1.0
|
N
|
I:CYS373
|
3.8
|
28.2
|
1.0
|
N
|
I:CYS359
|
3.9
|
30.9
|
1.0
|
CA
|
I:CYS373
|
4.1
|
25.4
|
1.0
|
CA
|
I:CYS359
|
4.2
|
27.1
|
1.0
|
CA
|
I:CYS370
|
4.6
|
23.8
|
1.0
|
CA
|
I:CYS356
|
4.6
|
22.2
|
1.0
|
CB
|
I:ILE372
|
4.7
|
26.8
|
1.0
|
O
|
I:HOH511
|
4.7
|
24.4
|
1.0
|
CB
|
I:PHE361
|
4.7
|
24.9
|
1.0
|
C
|
I:CYS373
|
4.7
|
28.8
|
1.0
|
C
|
I:CYS359
|
4.8
|
28.8
|
1.0
|
CB
|
I:SER358
|
4.8
|
20.3
|
1.0
|
N
|
I:GLU374
|
4.9
|
28.4
|
1.0
|
C
|
I:ILE372
|
4.9
|
26.9
|
1.0
|
N
|
I:PHE361
|
4.9
|
24.7
|
1.0
|
C
|
I:SER358
|
4.9
|
25.7
|
1.0
|
N
|
I:THR360
|
5.0
|
27.6
|
1.0
|
N
|
I:ILE372
|
5.0
|
34.8
|
1.0
|
|
Zinc binding site 4 out
of 4 in 9b12
Go back to
Zinc Binding Sites List in 9b12
Zinc binding site 4 out
of 4 in the Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1
Mono view
Stereo pair view
|
A full contact list of Zinc with other atoms in the Zn binding
site number 4 of Structure of Optineurin Bound to Hoip NZF1 Domain and M1-Linked Diubiquitin, Crystal Form 1 within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
J:Zn401
b:26.9
occ:1.00
|
SG
|
J:CYS359
|
2.3
|
28.3
|
1.0
|
SG
|
J:CYS370
|
2.3
|
23.7
|
1.0
|
SG
|
J:CYS373
|
2.3
|
25.8
|
1.0
|
SG
|
J:CYS356
|
2.3
|
24.0
|
1.0
|
CB
|
J:CYS356
|
3.0
|
25.6
|
1.0
|
CB
|
J:CYS370
|
3.2
|
20.9
|
1.0
|
CB
|
J:CYS359
|
3.4
|
19.5
|
1.0
|
CB
|
J:CYS373
|
3.4
|
20.2
|
1.0
|
N
|
J:CYS359
|
3.8
|
23.8
|
1.0
|
N
|
J:CYS373
|
3.8
|
27.2
|
1.0
|
CA
|
J:CYS359
|
4.1
|
24.6
|
1.0
|
CA
|
J:CYS373
|
4.1
|
20.7
|
1.0
|
CA
|
J:CYS356
|
4.5
|
20.2
|
1.0
|
CB
|
J:PHE361
|
4.6
|
23.1
|
1.0
|
CA
|
J:CYS370
|
4.7
|
20.9
|
1.0
|
C
|
J:CYS359
|
4.7
|
24.5
|
1.0
|
N
|
J:THR360
|
4.8
|
27.5
|
1.0
|
N
|
J:PHE361
|
4.8
|
22.5
|
1.0
|
CB
|
J:ILE372
|
4.8
|
19.4
|
1.0
|
C
|
J:CYS373
|
4.8
|
22.9
|
1.0
|
CB
|
J:SER358
|
4.8
|
27.2
|
0.6
|
CB
|
J:SER358
|
4.9
|
27.2
|
0.4
|
C
|
J:SER358
|
4.9
|
23.6
|
1.0
|
C
|
J:ILE372
|
4.9
|
25.7
|
1.0
|
N
|
J:GLU374
|
5.0
|
26.8
|
1.0
|
N
|
J:ILE372
|
5.0
|
21.7
|
1.0
|
|
Reference:
M.A.Michel,
S.Scutts,
D.Komander.
Linkage and Substrate Specificity Conferred By Nzf Ubiquitin Binding Domains To Be Published.
Page generated: Thu Oct 31 14:37:57 2024
|