Zinc in PDB 8vns: Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S

The structure of Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S, PDB code: 8vns was solved by C.Chang, Y.Gao, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.39 / 2.11
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	117.905, 117.905, 84.743, 90, 90, 120
R / Rfree (%)	22.2 / 25.2

The structure of Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S also contains other interesting chemical elements:

Manganese	(Mn)	2 atoms
Sodium	(Na)	2 atoms

The binding sites of Zinc atom in the Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S (pdb code 8vns). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 4 binding sites of Zinc where determined in the Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S, PDB code: 8vns:
Jump to Zinc binding site number: 1; 2; 3; 4;

Zinc binding site 1 out of 4 in the Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn601 b:33.6 occ:1.00 ND1 A:HIS134 2.1 33.2 1.0 SG A:CYS125 2.3 33.7 1.0 SG A:CYS138 2.3 31.6 1.0 SG A:CYS132 2.3 31.2 1.0 CE1 A:HIS134 2.9 30.3 1.0 CG A:HIS134 3.2 35.7 1.0 CB A:CYS132 3.2 29.0 1.0 CB A:CYS125 3.3 35.2 1.0 CB A:CYS138 3.3 24.7 1.0 CB A:HIS134 3.6 25.5 1.0 CA A:CYS132 4.0 36.3 1.0 N A:CYS138 4.1 32.6 1.0 NE2 A:HIS134 4.1 31.6 1.0 CD2 A:HIS134 4.2 28.2 1.0 N A:HIS134 4.2 29.9 1.0 CA A:CYS138 4.3 24.4 1.0 NH1 A:ARG122 4.5 30.1 1.0 N A:VAL133 4.6 28.1 1.0 CA A:HIS134 4.6 29.1 1.0 C A:CYS132 4.6 35.5 1.0 CA A:CYS125 4.7 28.0 1.0

Zinc binding site 2 out of 4 in the Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn602 b:30.3 occ:1.00 ND1 A:HIS110 2.1 26.4 1.0 SG A:CYS105 2.3 31.1 1.0 SG A:CYS41 2.3 34.1 1.0 SG A:CYS100 2.3 31.6 1.0 CE1 A:HIS110 3.0 30.7 1.0 CB A:CYS105 3.1 26.8 1.0 CB A:CYS100 3.2 25.6 1.0 CG A:HIS110 3.2 34.0 1.0 CB A:CYS41 3.2 31.7 1.0 CB A:HIS110 3.6 24.8 1.0 CA A:CYS41 3.7 27.3 1.0 NE2 A:HIS110 4.2 25.8 1.0 CD2 A:HIS110 4.3 30.8 1.0 N A:CYS41 4.5 37.3 1.0 CB A:ASN102 4.5 34.1 1.0 CA A:CYS105 4.6 30.4 1.0 CA A:CYS100 4.6 27.9 1.0 ND2 A:ASN102 4.7 34.1 1.0 CB A:ASN107 4.7 29.7 1.0 O A:HIS40 4.8 31.9 1.0 C A:CYS41 4.8 30.7 1.0 O A:ASN102 4.9 30.0 1.0 C A:HIS40 4.9 35.6 1.0 N A:TYR42 4.9 29.3 1.0

Zinc binding site 3 out of 4 in the Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn401 b:33.3 occ:1.00 ND1 B:HIS334 2.0 32.9 1.0 SG B:CYS325 2.3 34.0 1.0 SG B:CYS338 2.4 27.7 1.0 SG B:CYS332 2.4 30.8 1.0 CE1 B:HIS334 2.9 31.9 1.0 CG B:HIS334 3.2 34.4 1.0 CB B:CYS332 3.2 34.6 1.0 CB B:CYS338 3.2 37.3 1.0 CB B:CYS325 3.3 29.2 1.0 CB B:HIS334 3.6 32.1 1.0 N B:CYS338 4.0 31.3 1.0 CA B:CYS332 4.0 34.8 1.0 NE2 B:HIS334 4.0 30.1 1.0 CA B:CYS338 4.2 32.0 1.0 CD2 B:HIS334 4.2 32.2 1.0 N B:HIS334 4.3 36.8 1.0 NH1 B:ARG322 4.3 24.1 1.0 CA B:HIS334 4.6 31.8 1.0 N B:VAL333 4.6 34.9 1.0 C B:CYS332 4.6 39.7 1.0 CA B:CYS325 4.7 30.2 1.0

Zinc binding site 4 out of 4 in the Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Homing Endonuclease I-Ppoi-Dna Complex:Reaction at PH6.0 (K+ Mes) with 200 Mm MN2+ For 600S within 5.0Å range: probe atom residue distance (Å) B Occ B:Zn402 b:28.6 occ:1.00 ND1 B:HIS310 2.1 26.3 1.0 SG B:CYS241 2.3 25.9 1.0 SG B:CYS305 2.3 29.3 1.0 SG B:CYS300 2.3 28.5 1.0 CE1 B:HIS310 3.0 32.5 1.0 CB B:CYS305 3.1 23.5 1.0 CB B:CYS300 3.1 22.5 1.0 CG B:HIS310 3.2 32.7 1.0 CB B:CYS241 3.2 29.5 1.0 CB B:HIS310 3.6 30.5 1.0 CA B:CYS241 3.6 29.4 1.0 NE2 B:HIS310 4.2 30.5 1.0 CD2 B:HIS310 4.3 35.0 1.0 N B:CYS241 4.5 30.2 1.0 CA B:CYS305 4.6 25.9 1.0 CA B:CYS300 4.6 27.5 1.0 ND2 B:ASN302 4.7 28.0 1.0 CB B:ASN302 4.7 26.5 1.0 CB B:ASN307 4.7 30.2 1.0 C B:CYS241 4.8 30.7 1.0 O B:HIS240 4.9 28.5 1.0 N B:TYR242 4.9 28.1 1.0 O B:ASN302 5.0 26.0 1.0 C B:HIS240 5.0 29.9 1.0

C.Chang, Y.Gao. Homing Endonuclease I-Ppoi-Dna Complex:Reaction with 500 Um MG2+ For 160S To Be Published.