Zinc in PDB 8uqa: Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12- Residue Linker

All present enzymatic activity of Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12- Residue Linker:
2.3.2.23; 2.3.2.24; 2.3.2.27;

The structure of Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12- Residue Linker, PDB code: 8uqa was solved by Q.Hu, M.V.Botuyan, G.Mer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.18 / 2.05
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	102.953, 102.953, 87.231, 90, 90, 120
R / Rfree (%)	19.2 / 23.1

The structure of Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12- Residue Linker also contains other interesting chemical elements:

Chlorine	(Cl)	4 atoms
Sodium	(Na)	3 atoms

The binding sites of Zinc atom in the Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12- Residue Linker (pdb code 8uqa). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12- Residue Linker, PDB code: 8uqa:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12- Residue Linker


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12- Residue Linker within 5.0Å range: probe atom residue distance (Å) B Occ K:Zn4003 b:35.5 occ:1.00 ND1 K:HIS33 2.1 33.5 1.0 SG K:CYS31 2.3 43.2 1.0 SG K:CYS54 2.3 41.6 1.0 SG K:CYS51 2.4 40.5 1.0 CE1 K:HIS33 3.1 37.6 1.0 CG K:HIS33 3.1 35.2 1.0 CB K:CYS51 3.2 48.1 1.0 CB K:CYS31 3.2 44.4 1.0 CB K:HIS33 3.4 38.1 1.0 CB K:CYS54 3.5 39.5 1.0 N K:CYS54 3.8 45.7 1.0 NE2 K:HIS33 4.2 38.3 1.0 CA K:CYS54 4.2 49.7 1.0 CD2 K:HIS33 4.2 41.8 1.0 CB K:PHE53 4.4 36.1 1.0 N K:HIS33 4.4 34.4 1.0 CA K:CYS31 4.5 38.0 1.0 CA K:HIS33 4.5 41.1 1.0 O K:CYS31 4.6 36.5 1.0 CB K:ARG56 4.6 61.7 1.0 CA K:CYS51 4.6 55.9 1.0 C K:CYS31 4.7 34.6 1.0 C K:PHE53 4.8 43.3 1.0 C K:CYS54 4.8 46.5 1.0 N K:ARG55 4.9 47.4 1.0 CA K:PHE53 5.0 46.6 1.0 N K:PHE53 5.0 41.6 1.0 N K:ARG56 5.0 55.3 1.0

Zinc binding site 2 out of 2 in the Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12- Residue Linker


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12- Residue Linker within 5.0Å range: probe atom residue distance (Å) B Occ K:Zn4004 b:56.0 occ:1.00 SG K:CYS39 2.1 61.3 1.0 SG K:CYS19 2.4 74.9 1.0 SG K:CYS36 2.5 69.8 1.0 CB K:CYS39 2.9 55.6 1.0 CB K:CYS19 2.9 63.5 1.0 SG K:CYS16 2.9 61.5 1.0 N K:CYS19 3.5 65.1 1.0 CA K:CYS19 3.8 74.0 1.0 O K:HOH5241 3.9 60.5 1.0 CB K:CYS36 3.9 60.4 1.0 CA K:CYS39 4.0 59.5 1.0 N K:CYS39 4.1 57.6 1.0 CB K:CYS16 4.2 62.8 1.0 CB K:ILE18 4.3 61.3 1.0 N K:CYS36 4.3 59.1 1.0 C K:ILE18 4.5 64.9 1.0 CG2 K:ILE18 4.6 63.3 1.0 CA K:CYS36 4.7 59.2 1.0 O K:CYS36 4.7 56.9 1.0 CA K:ILE18 4.9 56.1 1.0 C K:CYS19 4.9 77.4 1.0

Q.Hu, M.V.Botuyan, G.Mer. Crystal Structure of RNF168 (Ring)-UBCH5C Fused to H2A-H2B Via A 12-Residue Linker To Be Published.