Zinc in PDB 8ukr: Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry

Enzymatic activity of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry

All present enzymatic activity of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry:
2.7.7.6;

Protein crystallography data

The structure of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry, PDB code: 8ukr was solved by P.Hou, J.Oh, D.Wang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.96 / 3.78
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 159.617, 222.632, 190.697, 90, 97.69, 90
R / Rfree (%) 26.1 / 31

Other elements in 8ukr:

The structure of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry also contains other interesting chemical elements:

Magnesium (Mg) 1 atom

Zinc Binding Sites:

The binding sites of Zinc atom in the Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry (pdb code 8ukr). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 8 binding sites of Zinc where determined in the Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry, PDB code: 8ukr:
Jump to Zinc binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Zinc binding site 1 out of 8 in 8ukr

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Zinc binding site 1 out of 8 in the Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry


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Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1801

b:342.5
occ:1.00
SG A:CYS110 2.3 245.7 1.0
SG A:CYS167 2.3 285.2 1.0
SG A:CYS107 2.3 225.2 1.0
SG A:CYS148 2.3 278.9 1.0
CB A:CYS167 3.2 241.2 1.0
CB A:CYS110 3.3 219.1 1.0
CB A:CYS107 3.4 212.7 1.0
CB A:CYS148 3.5 249.7 1.0
O A:ASN169 3.6 276.8 1.0
N A:GLY168 3.8 331.0 1.0
CA A:CYS167 3.9 241.5 1.0
N A:CYS110 4.1 222.2 1.0
C A:CYS167 4.2 267.8 1.0
N A:CYS148 4.2 211.4 1.0
N A:ASN169 4.3 317.0 1.0
CA A:CYS110 4.3 219.7 1.0
CA A:CYS148 4.4 218.9 1.0
C A:ASN169 4.7 274.2 1.0
CA A:CYS107 4.8 204.7 1.0
CA A:GLY168 4.8 329.2 1.0
O A:CYS148 4.9 220.4 1.0

Zinc binding site 2 out of 8 in 8ukr

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Zinc binding site 2 out of 8 in the Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry


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Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Zn1802

b:208.4
occ:1.00
NE2 A:HIS80 2.2 256.1 1.0
SG A:CYS77 2.3 153.5 1.0
SG A:CYS67 2.3 231.7 1.0
SG A:CYS70 2.3 210.6 1.0
CE1 A:HIS80 3.0 260.4 1.0
CB A:CYS70 3.2 178.0 1.0
CB A:CYS77 3.2 145.1 1.0
O A:LEU58 3.2 216.5 1.0
CD2 A:HIS80 3.2 247.5 1.0
CB A:CYS67 3.5 212.7 1.0
O A:ARG57 3.6 221.2 1.0
N A:GLN68 3.9 215.3 1.0
CA A:CYS77 4.1 157.3 1.0
ND1 A:HIS80 4.1 259.9 1.0
C A:LEU58 4.2 212.3 1.0
N A:GLY79 4.2 182.1 1.0
CG A:HIS80 4.3 249.5 1.0
CA A:CYS67 4.3 203.3 1.0
CA A:CYS70 4.4 196.5 1.0
N A:CYS70 4.5 222.4 1.0
C A:CYS67 4.5 211.2 1.0
C A:CYS77 4.6 173.0 1.0
CA A:GLY79 4.6 166.3 1.0
CD A:PRO78 4.7 187.0 1.0
CA A:LEU58 4.7 207.9 1.0
C A:ARG57 4.7 225.6 1.0
N A:PRO78 4.7 191.6 1.0
CA A:GLN68 4.8 200.8 1.0
O A:GLN68 4.8 163.4 1.0
CB A:GLN68 4.8 188.6 1.0
C A:GLN68 4.9 190.0 1.0

Zinc binding site 3 out of 8 in 8ukr

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Zinc binding site 3 out of 8 in the Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry


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Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 3 of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Zn1301

b:216.8
occ:1.00
SG B:CYS1182 2.3 228.6 1.0
SG B:CYS1163 2.3 183.2 1.0
SG B:CYS1185 2.3 213.1 1.0
SG B:CYS1166 2.3 145.7 1.0
CB B:CYS1166 2.9 159.2 1.0
N B:CYS1166 3.1 194.7 1.0
CB B:CYS1163 3.2 171.2 1.0
CB B:CYS1185 3.3 197.1 1.0
CB B:CYS1182 3.5 203.0 1.0
CA B:CYS1166 3.5 189.3 1.0
CA B:CYS1185 3.8 168.8 1.0
N B:CYS1185 4.1 167.2 1.0
C B:ILE1165 4.1 175.4 1.0
CB B:ILE1165 4.1 170.8 1.0
O B:LYS1183 4.4 237.7 1.0
CA B:ILE1165 4.5 164.7 1.0
N B:ILE1165 4.6 161.1 1.0
CA B:CYS1163 4.6 157.4 1.0
CB B:THR1170 4.7 161.0 1.0
CG2 B:ILE1165 4.8 153.9 1.0
CA B:CYS1182 4.8 184.8 1.0
C B:GLY1184 4.9 159.9 1.0
C B:CYS1166 4.9 174.8 1.0
C B:CYS1182 4.9 195.2 1.0
C B:CYS1163 4.9 172.6 1.0
OG1 B:THR1170 5.0 173.6 1.0
O B:LEU1168 5.0 164.3 1.0

Zinc binding site 4 out of 8 in 8ukr

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Zinc binding site 4 out of 8 in the Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry


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Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 4 of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Zn401

b:158.5
occ:1.00
SG C:CYS92 2.3 60.8 1.0
SG C:CYS88 2.3 71.8 1.0
SG C:CYS86 2.3 89.5 1.0
SG C:CYS95 2.3 104.0 1.0
N C:CYS92 2.8 105.3 1.0
CB C:CYS92 3.1 96.6 1.0
CB C:CYS95 3.1 107.3 1.0
CB C:CYS88 3.3 63.3 1.0
CB C:CYS86 3.3 139.4 1.0
CA C:CYS92 3.4 91.8 1.0
C C:HIS91 3.8 125.6 1.0
O C:CYS92 4.1 74.2 1.0
CA C:HIS91 4.1 114.2 1.0
C C:CYS92 4.2 58.8 1.0
N C:CYS88 4.2 98.3 1.0
CA C:CYS88 4.3 65.1 1.0
CA C:CYS95 4.3 103.6 1.0
N C:CYS95 4.3 102.1 1.0
CA C:SER83 4.6 71.1 1.0
CA C:CYS86 4.6 147.2 1.0
O C:HIS91 4.7 129.3 1.0
C C:CYS86 4.8 143.6 1.0
O C:SER83 4.8 113.6 1.0
C C:CYS88 4.8 102.3 1.0
N C:HIS91 4.8 128.4 1.0
CB C:SER83 4.9 90.7 1.0

Zinc binding site 5 out of 8 in 8ukr

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Zinc binding site 5 out of 8 in the Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry


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Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 5 of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn201

b:216.8
occ:1.00
SG I:CYS7 2.3 116.7 1.0
SG I:CYS10 2.3 234.4 1.0
SG I:CYS32 2.3 149.8 1.0
SG I:CYS29 2.3 224.2 1.0
N I:CYS32 2.8 134.5 1.0
CB I:CYS32 2.9 137.6 1.0
CB I:THR31 3.2 171.1 1.0
C I:THR31 3.3 139.2 1.0
CB I:CYS10 3.3 176.2 1.0
CA I:CYS32 3.4 149.2 1.0
CB I:CYS29 3.6 156.3 1.0
CA I:THR31 3.7 153.8 1.0
CB I:ASN12 3.8 146.9 1.0
CG2 I:THR31 3.9 158.6 1.0
CB I:CYS7 3.9 112.9 1.0
O I:THR31 4.1 133.2 1.0
N I:THR31 4.1 147.1 1.0
OG1 I:THR31 4.1 183.7 1.0
N I:ASN12 4.2 120.4 1.0
CA I:CYS10 4.3 166.8 1.0
N I:CYS10 4.4 150.9 1.0
ND2 I:ASN12 4.4 148.8 1.0
C I:CYS32 4.5 152.6 1.0
O I:CYS32 4.5 157.4 1.0
C I:CYS10 4.5 176.8 1.0
CG I:ASN12 4.5 150.8 1.0
CA I:ASN12 4.6 130.5 1.0
N I:ASN11 4.6 146.6 1.0
O I:ASN12 4.9 133.0 1.0
CA I:CYS29 4.9 156.8 1.0
O I:CYS7 5.0 159.5 1.0

Zinc binding site 6 out of 8 in 8ukr

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Zinc binding site 6 out of 8 in the Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 6 of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry within 5.0Å range:
probe atom residue distance (Å) B Occ
I:Zn202

b:287.5
occ:1.00
N I:CYS78 2.3 229.6 1.0
SG I:CYS103 2.3 271.4 1.0
SG I:CYS78 2.3 223.1 1.0
SG I:CYS75 2.3 280.4 1.0
SG I:CYS106 2.3 204.0 1.0
CB I:CYS78 2.9 205.2 1.0
CA I:CYS78 3.0 209.3 1.0
CB I:CYS75 3.2 195.2 1.0
C I:LYS77 3.3 257.0 1.0
CB I:CYS103 3.4 224.3 1.0
CB I:LYS77 3.6 210.8 1.0
CA I:LYS77 3.7 221.5 1.0
O I:SER105 3.7 238.5 1.0
CB I:CYS106 3.8 208.2 1.0
N I:LYS77 3.8 210.5 1.0
CB I:HIS108 3.8 196.9 1.0
ND1 I:HIS108 4.0 201.9 1.0
O I:CYS75 4.3 223.3 1.0
O I:LYS77 4.3 270.5 1.0
C I:CYS78 4.3 213.0 1.0
C I:CYS75 4.4 226.6 1.0
CA I:CYS75 4.4 196.9 1.0
CG I:HIS108 4.4 196.6 1.0
N I:HIS79 4.5 212.1 1.0
N I:HIS108 4.6 193.6 1.0
O I:HIS108 4.7 187.5 1.0
CA I:CYS103 4.7 187.8 1.0
CA I:HIS108 4.7 193.2 1.0
C I:PRO76 4.7 210.7 1.0
CG I:LYS77 4.8 212.4 1.0
CA I:CYS106 4.8 206.9 1.0
C I:SER105 4.8 233.4 1.0
C I:CYS106 4.9 202.9 1.0

Zinc binding site 7 out of 8 in 8ukr

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Zinc binding site 7 out of 8 in the Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry


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Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 7 of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry within 5.0Å range:
probe atom residue distance (Å) B Occ
J:Zn101

b:115.9
occ:1.00
SG J:CYS10 2.3 117.2 1.0
SG J:CYS7 2.3 54.0 1.0
SG J:CYS46 2.3 40.6 1.0
SG J:CYS45 2.3 145.0 1.0
CB J:CYS10 2.8 69.1 1.0
N J:CYS46 3.0 115.5 1.0
CB J:CYS7 3.3 66.2 1.0
CB J:CYS45 3.4 104.7 1.0
CB J:CYS46 3.5 113.1 1.0
CA J:CYS46 3.5 112.2 1.0
OG J:SER9 3.5 77.2 1.0
N J:CYS10 3.6 63.7 1.0
C J:CYS45 3.6 106.2 1.0
CB J:ARG43 3.8 108.6 1.0
CA J:CYS10 3.8 88.9 1.0
CA J:CYS45 4.0 91.0 1.0
NH1 J:ARG43 4.3 108.0 1.0
CZ J:ARG43 4.3 119.1 1.0
CG J:ARG43 4.4 125.2 1.0
O J:CYS45 4.4 95.5 1.0
N J:CYS45 4.5 87.4 1.0
NH2 J:ARG43 4.5 122.1 1.0
NH2 J:ARG48 4.7 58.6 1.0
NE J:ARG43 4.7 121.7 1.0
CA J:CYS7 4.8 102.1 1.0
O J:ARG43 4.8 97.1 1.0
C J:SER9 4.8 75.2 1.0
CB J:SER9 4.9 89.5 1.0

Zinc binding site 8 out of 8 in 8ukr

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Zinc binding site 8 out of 8 in the Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 8 of Rna Polymerase II Elongation Complex with Fapy-Dg Lesion Soaking with Atp Before Chemistry within 5.0Å range:
probe atom residue distance (Å) B Occ
L:Zn101

b:369.9
occ:1.00
SG L:CYS48 2.3 347.5 1.0
SG L:CYS34 2.3 429.0 1.0
SG L:CYS31 2.3 343.4 1.0
SG L:CYS51 2.3 328.7 1.0
CB L:CYS31 3.1 277.9 1.0
CB L:CYS51 3.4 323.7 1.0
CB L:CYS34 3.5 387.4 1.0
O L:HIS53 3.5 237.4 1.0
N L:CYS34 3.8 400.8 1.0
CB L:CYS48 3.8 335.9 1.0
O L:ILE55 3.9 182.5 1.0
CA L:CYS34 4.3 395.3 1.0
N L:CYS48 4.3 319.4 1.0
O L:CYS51 4.4 318.4 1.0
CA L:CYS51 4.4 319.4 1.0
CA L:CYS31 4.5 286.7 1.0
CA L:CYS48 4.6 321.9 1.0
C L:CYS51 4.7 306.7 1.0
C L:HIS53 4.7 233.2 1.0
N L:CYS51 4.7 329.6 1.0
C L:CYS31 4.8 322.1 1.0
CB L:GLU33 4.8 313.3 1.0
C L:GLU33 4.8 327.0 1.0
O L:CYS48 4.9 325.4 1.0
N L:GLU33 5.0 321.1 1.0
O L:CYS31 5.0 336.2 1.0

Reference:

S.Gao, P.Hou, J.Oh, D.Wang, M.M.Greenberg. Molecular Mechanism of Rna Polymerase II Transcriptional Mutagenesis By the Epimerizable Dna Lesion, Fapy·Dg. J.Am.Chem.Soc. V. 146 6274 2024.
ISSN: ESSN 1520-5126
PubMed: 38393762
DOI: 10.1021/JACS.3C14476
Page generated: Thu Oct 31 12:19:13 2024

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