Zinc in PDB 8sj1: Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp

All present enzymatic activity of Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp:
2.7.7.86;

The structure of Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp, PDB code: 8sj1 was solved by S.Wu, J.Sohn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.74 / 2.81
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	77.184, 97.58, 142.283, 90, 90, 90
R / Rfree (%)	18.8 / 24

The structure of Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Zinc atom in the Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp (pdb code 8sj1). This binding sites where shown within 5.0 Angstroms radius around Zinc atom.
In total 2 binding sites of Zinc where determined in the Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp, PDB code: 8sj1:
Jump to Zinc binding site number: 1; 2;

Zinc binding site 1 out of 2 in the Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 1 of Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp within 5.0Å range: probe atom residue distance (Å) B Occ A:Zn602 b:49.5 occ:1.00 NE2 A:HIS378 2.1 36.9 1.0 SG A:CYS384 2.3 35.3 1.0 SG A:CYS385 2.3 36.5 1.0 SG A:CYS392 2.3 34.6 1.0 CD2 A:HIS378 2.9 34.5 1.0 CE1 A:HIS378 3.2 34.4 1.0 CB A:CYS392 3.4 39.8 1.0 CB A:CYS385 3.5 36.5 1.0 CB A:CYS384 3.7 31.1 1.0 C A:CYS384 3.8 35.6 1.0 N A:CYS392 3.9 36.1 1.0 O A:CYS384 4.0 36.9 1.0 N A:CYS385 4.0 37.0 1.0 CG A:HIS378 4.1 34.6 1.0 CA A:CYS392 4.2 38.9 1.0 ND1 A:HIS378 4.2 33.9 1.0 CA A:CYS384 4.3 33.3 1.0 CA A:CYS385 4.3 36.9 1.0 C A:CYS392 4.7 42.6 1.0 NE A:ARG394 4.8 31.9 1.0 C A:LYS391 4.8 40.7 1.0 O A:ALA390 4.9 40.1 1.0 O A:CYS392 5.0 43.4 1.0

Zinc binding site 2 out of 2 in the Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp


Mono view


Stereo pair view

A full contact list of Zinc with other atoms in the Zn binding site number 2 of Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp within 5.0Å range: probe atom residue distance (Å) B Occ C:Zn602 b:40.0 occ:1.00 NE2 C:HIS378 2.1 38.9 1.0 SG C:CYS385 2.3 36.5 1.0 SG C:CYS384 2.3 39.2 1.0 SG C:CYS392 2.3 42.7 1.0 CD2 C:HIS378 3.0 36.9 1.0 CE1 C:HIS378 3.2 36.2 1.0 CB C:CYS385 3.2 36.6 1.0 CB C:CYS392 3.4 39.9 1.0 CB C:CYS384 3.6 33.2 1.0 N C:CYS385 3.7 38.5 1.0 C C:CYS384 3.8 36.4 1.0 N C:CYS392 3.9 38.9 1.0 CA C:CYS385 4.1 39.1 1.0 CA C:CYS392 4.2 39.7 1.0 CG C:HIS378 4.2 35.5 1.0 O C:CYS384 4.2 36.0 1.0 CA C:CYS384 4.2 35.8 1.0 ND1 C:HIS378 4.3 34.4 1.0 C C:CYS392 4.7 42.9 1.0 C C:LYS391 4.8 42.3 1.0 NH1 C:ARG394 4.8 38.3 1.0 O C:ALA390 4.8 42.9 1.0 O C:CYS392 4.9 45.1 1.0 CD C:ARG394 5.0 39.1 1.0

S.Wu, J.Sohn. Structure of Ternary Complex of Cgas with Dsdna and Bound 3'-Datp To Be Published.